Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia
A total of 32 lactic acid bacteria (LAB) were isolated from 13 honey samples commercially marketed in Malaysia, and six strains identified as (Lactobacilus acidophilus) by API CHL50 were evaluated for their antibacterial activities against multiple antibiotic resistant (Staphylococcus epidermis), (B...
Saved in:
| id |
my-usim-ddms-12430 |
|---|---|
| record_format |
uketd_dc |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
| language |
English |
| topic |
Lactic acid bacteria Honey |
| spellingShingle |
Lactic acid bacteria Honey Mohamed Mustafa Aween Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia |
| description |
A total of 32 lactic acid bacteria (LAB) were isolated from 13 honey samples commercially marketed in Malaysia, and six strains identified as (Lactobacilus acidophilus) by API CHL50 were evaluated for their antibacterial activities against multiple antibiotic resistant (Staphylococcus epidermis), (Bacilus subtilis), (Salmonella typhimurium), (Escherichia coli), (Klebsiella pneumoniae), (Shigella sonnei), (Serretia marcescens) and (Enterobacter aerogenes). Using the overlay agar method the inhibition zone varies with target bacteria which were (S. aureus) 25 to 32 mm, (Staphycoloccus epidermis) 14 to 22 mm, (Bacillus subtilis) 12 to19 mm, (Salmonella typhimurium) 23 to 30 mm, (E. coli) 7 to 18 mm, (Klebsiella pneumoniae) 13.5 to 21.5 mm, (S. sonnei) 9 to 22 mm, (Serretia marcescens) 7.5 to 18 mm, and (E. aerogenes) 10 to 18 mm after 24 h incubation at 30 degree celcius. The effects of temperature, protolithic enzymes and pH on the antibacterial activity of the supernatants were evaluated. The supernatant was stable at 90 degree celcius and 121 degree celcius for 1 hour. Treatment with proteinase K and RNase II maintained the antimicrobial activity of all the supernatants ecxept H006-A from Libyan honey and H010-G from New Zealand honey. All the supernatants showed antimicrobial activities against target bacteria at pH 3 and pH 5 but not pH 6 except against (Enterobacter aerogenes) within 48 h incubation at 30 degree celcius. (Staphylococcus aureus) was not inhibited by H006-A isolated from Libyan honey and H008-d isolated from Malaysian honey at pH 5, compared to supernatants from other (L. acidophilus) isolates. Result from PCR identified LAB isolates as uncultured bacteria. Eight primers were used for RAPD and primer P69 highly discriminated the six isolates. The results from RAPD suggested that the strains of (Lactobacillus acidophilus were different from each other depending on the source of honey. |
| format |
Thesis |
| author |
Mohamed Mustafa Aween |
| author_facet |
Mohamed Mustafa Aween |
| author_sort |
Mohamed Mustafa Aween |
| title |
Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia |
| title_short |
Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia |
| title_full |
Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia |
| title_fullStr |
Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia |
| title_full_unstemmed |
Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia |
| title_sort |
isolation, characterization and antibacterial activity of lactic acid bacteria from commercial honey marketed in malaysia |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
https://oarep.usim.edu.my/bitstreams/0d25cce5-a0a9-4131-aa1f-55f5b4966acf/download https://oarep.usim.edu.my/bitstreams/5426452e-5530-4ff5-87f4-598f2642482e/download https://oarep.usim.edu.my/bitstreams/6c72d7da-05b8-4520-ace5-bd21fe35f5d9/download https://oarep.usim.edu.my/bitstreams/53de37a1-7f56-4d50-bc16-9b35a8eba6f1/download https://oarep.usim.edu.my/bitstreams/1a6b308d-35b6-47dc-a404-9fac8e135f26/download https://oarep.usim.edu.my/bitstreams/f8321a35-30cf-48cc-8df4-502866308bcb/download https://oarep.usim.edu.my/bitstreams/5940f95c-adc8-487b-ba08-3e245735357b/download https://oarep.usim.edu.my/bitstreams/bf59b6fd-6c32-4794-9235-ab9830b79e4f/download https://oarep.usim.edu.my/bitstreams/54023ce3-cb10-4d9d-bb02-f810911f6b12/download https://oarep.usim.edu.my/bitstreams/beaef6b1-bc3f-484c-8203-4120749da7ea/download https://oarep.usim.edu.my/bitstreams/c05bbea2-120f-4464-ac2d-92e8fa6daa23/download |
| _version_ |
1812444867615260672 |
| spelling |
my-usim-ddms-124302024-05-29T20:10:32Z Isolation, characterization and antibacterial activity of Lactic Acid Bacteria from commercial honey marketed in Malaysia Mohamed Mustafa Aween A total of 32 lactic acid bacteria (LAB) were isolated from 13 honey samples commercially marketed in Malaysia, and six strains identified as (Lactobacilus acidophilus) by API CHL50 were evaluated for their antibacterial activities against multiple antibiotic resistant (Staphylococcus epidermis), (Bacilus subtilis), (Salmonella typhimurium), (Escherichia coli), (Klebsiella pneumoniae), (Shigella sonnei), (Serretia marcescens) and (Enterobacter aerogenes). Using the overlay agar method the inhibition zone varies with target bacteria which were (S. aureus) 25 to 32 mm, (Staphycoloccus epidermis) 14 to 22 mm, (Bacillus subtilis) 12 to19 mm, (Salmonella typhimurium) 23 to 30 mm, (E. coli) 7 to 18 mm, (Klebsiella pneumoniae) 13.5 to 21.5 mm, (S. sonnei) 9 to 22 mm, (Serretia marcescens) 7.5 to 18 mm, and (E. aerogenes) 10 to 18 mm after 24 h incubation at 30 degree celcius. The effects of temperature, protolithic enzymes and pH on the antibacterial activity of the supernatants were evaluated. The supernatant was stable at 90 degree celcius and 121 degree celcius for 1 hour. Treatment with proteinase K and RNase II maintained the antimicrobial activity of all the supernatants ecxept H006-A from Libyan honey and H010-G from New Zealand honey. All the supernatants showed antimicrobial activities against target bacteria at pH 3 and pH 5 but not pH 6 except against (Enterobacter aerogenes) within 48 h incubation at 30 degree celcius. (Staphylococcus aureus) was not inhibited by H006-A isolated from Libyan honey and H008-d isolated from Malaysian honey at pH 5, compared to supernatants from other (L. acidophilus) isolates. Result from PCR identified LAB isolates as uncultured bacteria. Eight primers were used for RAPD and primer P69 highly discriminated the six isolates. The results from RAPD suggested that the strains of (Lactobacillus acidophilus were different from each other depending on the source of honey. Universiti Sains Islam Malaysia 2012 Thesis en https://oarep.usim.edu.my/handle/123456789/12430 https://oarep.usim.edu.my/bitstreams/9b1b20de-6753-4ca0-8e79-b7e96a1c094e/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/0d25cce5-a0a9-4131-aa1f-55f5b4966acf/download 11e0b07845de91149e616dda4160113d https://oarep.usim.edu.my/bitstreams/5426452e-5530-4ff5-87f4-598f2642482e/download d97f261fcb5c3feab982df4da8d98af9 https://oarep.usim.edu.my/bitstreams/6c72d7da-05b8-4520-ace5-bd21fe35f5d9/download 6339f76f162df0fb183cbb7b379a40fc https://oarep.usim.edu.my/bitstreams/53de37a1-7f56-4d50-bc16-9b35a8eba6f1/download d6a91ee2bd3500be78fc8a2a0d94f541 https://oarep.usim.edu.my/bitstreams/1a6b308d-35b6-47dc-a404-9fac8e135f26/download 800e8027adce1db2399499105d954695 https://oarep.usim.edu.my/bitstreams/f8321a35-30cf-48cc-8df4-502866308bcb/download 828bdb7704a1a0f38e497f06f0bbbba7 https://oarep.usim.edu.my/bitstreams/5940f95c-adc8-487b-ba08-3e245735357b/download 3620eea18611b89761361c7b1172f7d8 https://oarep.usim.edu.my/bitstreams/bf59b6fd-6c32-4794-9235-ab9830b79e4f/download bfb4e80dfc7b0cff8024c9ab81843e59 https://oarep.usim.edu.my/bitstreams/54023ce3-cb10-4d9d-bb02-f810911f6b12/download fc2ccb462b7120bbb56c98876526d044 https://oarep.usim.edu.my/bitstreams/beaef6b1-bc3f-484c-8203-4120749da7ea/download 7ab46d0187f841e8f9867c08c8b598b6 https://oarep.usim.edu.my/bitstreams/c05bbea2-120f-4464-ac2d-92e8fa6daa23/download 39aa675927d41d1caae02a15aa7a0245 https://oarep.usim.edu.my/bitstreams/74c53a83-3b07-40bf-97a4-b13a803a4400/download f00d604b76cf19e478e9168bd80936db https://oarep.usim.edu.my/bitstreams/5f05aad5-d598-476a-b20e-47a9b9fdd8ee/download 088be42b53e84858382d070d3f2a973b https://oarep.usim.edu.my/bitstreams/ce85e026-6ba2-4829-8863-ea99549d0684/download 33f4f15a16a9843faf6a25d4f387b6fd https://oarep.usim.edu.my/bitstreams/ef4d72bf-4864-4371-add4-0e8c4fd2e486/download 2228e977ebea8966e27929f43e39cb67 https://oarep.usim.edu.my/bitstreams/bf2b0226-bca1-4b38-a7c6-af4be349943d/download 24e648839aa31918bceb936aff170ec0 https://oarep.usim.edu.my/bitstreams/142d356e-ba16-4a71-af09-a4c0ffce59a0/download cc9067c2ee470dc248b14b194209a34e https://oarep.usim.edu.my/bitstreams/6b8f1839-0471-49d3-965c-973d132c7d6f/download 429079e52f342c49301a02e207bfde06 https://oarep.usim.edu.my/bitstreams/d3f40d4e-31fc-474b-a14a-76bfd9b25ec0/download 24d68299ae13852334a122ea657bbaaa https://oarep.usim.edu.my/bitstreams/3e160000-d4f9-4ba0-8324-6a04f81b92c1/download 1bb607118047afc5c385b82385dd931f https://oarep.usim.edu.my/bitstreams/b469e545-4d53-498a-9bea-d435aa79bcdc/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/996340d9-e24a-4b30-96ce-64baea8a546e/download 91ce7a13557f30697b7729d573c57ace Lactic acid bacteria Honey |