Study on Biological and Phytochemical Properties of Euphorbia hirta

Euphorbia hirta is an annual broad-leaved herb and widely used as traditional medicine to treat various ailments. In this study, the antimicrobial activity of the E. hirta extracts (whole plants, flowers, stems, leaves, and roots) was evaluated via agarwell diffusion and microdilution tests. General...

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Main Author:	Nur Aishah Binti Abdul Wahab
Format:	Thesis
Language:	en_US
Subjects:	Euphorbia hirta Biotechnology. Plant breeding.
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id	my-usim-ddms-12549
record_format	uketd_dc
spelling	my-usim-ddms-125492024-05-29T04:19:13Z Study on Biological and Phytochemical Properties of Euphorbia hirta Nur Aishah Binti Abdul Wahab Euphorbia hirta is an annual broad-leaved herb and widely used as traditional medicine to treat various ailments. In this study, the antimicrobial activity of the E. hirta extracts (whole plants, flowers, stems, leaves, and roots) was evaluated via agarwell diffusion and microdilution tests. Generally, the plant extracts exhibited the antimicrobial activity towards ten selected Gram positive and Gram negative bacteria, namely B. cereus, B. subtilis, E. faecalis, S. aureus, S. epidermidis, K. pneumoniae, S. typhimurium, S. dysentriae, S. sonnei and S. marcescens. Since E. hirta extracts showed the highest antimicrobial activity against S. epidermidis (Gram positive) and S. sonnei (Gram negative), further study on the combination effects and pharmacodynamic properties were carried out. The two different parts of plants combinations, which are flowers-stems, flowers-leaves, flowers-roots, stems-leaves, stems-roots and leaves-roots, each was used to treat the two selected bacterial strains. Synergism effect could be bserved only for the combination treatments towards S.epidermidis with the highest synergism effect (89%) showed by flowers-leaves and stems-leaves combination. The E. hirta extracts also exhibited the bactericidal activity in time-kill study with a significant of post-antibiotic effects (PAE) value towards those two strains of bacteria. In preliminary screening, each fresh parts of E. hirta (flowers, stems, leaves and roots) showed a potential anti-quorum sensing (anti-QS) activity. Further screening on E. hirta extracts via agar-well diffusion test also exhibited the wide variation of anti-QS activity. The highest anti-QS activity was recorded for leaves and flowers extracts with the lowest of minimum QS inhibition concentration value. Phytochemical screening of E. hirta extracts by standard chemical test revealed the presence of carbohydrates, lipids, protein, flavonoids, alkaloids, saponins, resins, steroids, acidic compounds, tannins, glycosides, phenols and terpenoids. The quantitative phytochemical assays via GC-MS indicated that the E. hirra rich with fatty acids and terpenoids compounds. Universiti Sains Islam Malaysia 2014-10 Thesis en_US https://oarep.usim.edu.my/handle/123456789/12549 https://oarep.usim.edu.my/bitstreams/ededeee7-b7e6-4117-ae52-a2d90c661ad8/download 8a4605be74aa9ea9d79846c1fba20a33 Euphorbia hirta Biotechnology. Plant breeding. Herb, traditional medicine.
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	en_US
topic	Euphorbia hirta Biotechnology. Plant breeding. Euphorbia hirta
spellingShingle	Euphorbia hirta Biotechnology. Plant breeding. Euphorbia hirta Nur Aishah Binti Abdul Wahab Study on Biological and Phytochemical Properties of Euphorbia hirta
description	Euphorbia hirta is an annual broad-leaved herb and widely used as traditional medicine to treat various ailments. In this study, the antimicrobial activity of the E. hirta extracts (whole plants, flowers, stems, leaves, and roots) was evaluated via agarwell diffusion and microdilution tests. Generally, the plant extracts exhibited the antimicrobial activity towards ten selected Gram positive and Gram negative bacteria, namely B. cereus, B. subtilis, E. faecalis, S. aureus, S. epidermidis, K. pneumoniae, S. typhimurium, S. dysentriae, S. sonnei and S. marcescens. Since E. hirta extracts showed the highest antimicrobial activity against S. epidermidis (Gram positive) and S. sonnei (Gram negative), further study on the combination effects and pharmacodynamic properties were carried out. The two different parts of plants combinations, which are flowers-stems, flowers-leaves, flowers-roots, stems-leaves, stems-roots and leaves-roots, each was used to treat the two selected bacterial strains. Synergism effect could be bserved only for the combination treatments towards S.epidermidis with the highest synergism effect (89%) showed by flowers-leaves and stems-leaves combination. The E. hirta extracts also exhibited the bactericidal activity in time-kill study with a significant of post-antibiotic effects (PAE) value towards those two strains of bacteria. In preliminary screening, each fresh parts of E. hirta (flowers, stems, leaves and roots) showed a potential anti-quorum sensing (anti-QS) activity. Further screening on E. hirta extracts via agar-well diffusion test also exhibited the wide variation of anti-QS activity. The highest anti-QS activity was recorded for leaves and flowers extracts with the lowest of minimum QS inhibition concentration value. Phytochemical screening of E. hirta extracts by standard chemical test revealed the presence of carbohydrates, lipids, protein, flavonoids, alkaloids, saponins, resins, steroids, acidic compounds, tannins, glycosides, phenols and terpenoids. The quantitative phytochemical assays via GC-MS indicated that the E. hirra rich with fatty acids and terpenoids compounds.
format	Thesis
author	Nur Aishah Binti Abdul Wahab
author_facet	Nur Aishah Binti Abdul Wahab
author_sort	Nur Aishah Binti Abdul Wahab
title	Study on Biological and Phytochemical Properties of Euphorbia hirta
title_short	Study on Biological and Phytochemical Properties of Euphorbia hirta
title_full	Study on Biological and Phytochemical Properties of Euphorbia hirta
title_fullStr	Study on Biological and Phytochemical Properties of Euphorbia hirta
title_full_unstemmed	Study on Biological and Phytochemical Properties of Euphorbia hirta
title_sort	study on biological and phytochemical properties of euphorbia hirta
granting_institution	Universiti Sains Islam Malaysia
_version_	1812444718937669632

Study on Biological and Phytochemical Properties of Euphorbia hirta

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