Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis

In this study, the extraction using different solvents (chloroform, methanol and hexane) with reagent N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and 1% trimethyl- chlorosilane’ (TMCS) and their mixtures were used to extract biochemical compounds in three types of dates Kheseab (Tamar), Segae...

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Main Author:	Taffaha A. Arhouma
Format:	Thesis
Language:	English
Subjects:	Biochemical Compounds and Principal Components Analysis(PCA) Dates (Fruit) > Extraction (Chemistry) > Analysis Dates (Fruit) > Bioactive compounds BSTFA TMCS
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spelling	my-usim-ddms-126142024-05-29T18:08:20Z Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis Taffaha A. Arhouma In this study, the extraction using different solvents (chloroform, methanol and hexane) with reagent N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and 1% trimethyl- chlorosilane’ (TMCS) and their mixtures were used to extract biochemical compounds in three types of dates Kheseab (Tamar), Segae (Rutab) and Khalas (Tamar). The compounds were determined using Gas chromatography-mass spectrometry (GS-MS) and Liquid chromatography time-of-flight mass spectrometer (LC-TOF-MS). The results of GC-MS showed that the extraction using the mixture of methanol and chloroform (50% : 50%) extracted 16 compounds followed by methanol with hexane (50% : 50%) which extracted 15 compounds, and mixture of three solvents (33.3% : 33.3% : 33.3%)b14 compounds. While the other mixture of solvents, less number of compounds were extracted as compared with the previous mixtures. The results of LC- TOF-MS showed that the extraction using methanol and chloroform (50% : 50%) in Khalas (Tamar) was able to extract 27 compounds followed by Segae (Rutab) with 17 compounds while in Kheseab (Tamar) only 10 compounds were extracted. Principal Components Analysis (PCA) was performed on multivariate data of GS-MS results to study the relationship between compounds extracted with polarity of solvents mixture from the scores and loadings plots. Universiti Sains Islam Malaysia 2016-12 Thesis en https://oarep.usim.edu.my/handle/123456789/12614 https://oarep.usim.edu.my/bitstreams/33eca94a-8b2a-433d-895c-c2b30162470d/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/05f1f44f-d05a-4234-bc4e-549313943a45/download 786b9556cb83cb91b5d653210252130c https://oarep.usim.edu.my/bitstreams/ff0dbf8a-7f0b-4bdc-8887-c66478db212f/download 827c4131272ef9e31c3b20f57f602e08 https://oarep.usim.edu.my/bitstreams/0aa3b158-1f35-4b74-b233-00d7f2f674db/download 9092d3385cf51e995b69f6d1bec7bf04 https://oarep.usim.edu.my/bitstreams/46b80bdd-2236-40b4-b5cb-69c644c85521/download 3c8532fddf3a4467d6f51b1ab2707b14 https://oarep.usim.edu.my/bitstreams/30608ab2-daf6-4f63-9887-4ba1fc1ce425/download 71c6cc3e51534bfb179b9d3b9d2292a1 https://oarep.usim.edu.my/bitstreams/333e9d42-fd13-4a60-9d94-2ec9457a462e/download 2d485d7980bb44718681dd874e35bb4a https://oarep.usim.edu.my/bitstreams/bf791a21-532d-4315-a640-dc1899733c84/download 725ca3a3fb9596d6eae7b4909512e4fa https://oarep.usim.edu.my/bitstreams/ef42ec5b-2101-4fb0-bd53-8dc42ee6a1ed/download 860046435d2d400651175e5eaa2e1c12 https://oarep.usim.edu.my/bitstreams/44030a25-35b2-4cf2-9805-4966c92d18a6/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/932aceab-8ae0-4463-9c6f-3ed14ea8e6b4/download 01b4fd40100d2cc3e3ef44ad97de725e https://oarep.usim.edu.my/bitstreams/1776555e-fab5-4ac0-938d-b37584a06b98/download d87ba8e0604205b0f13b421946a0364f https://oarep.usim.edu.my/bitstreams/b78432c2-8c51-444e-8126-a43da07e0b9b/download da07cd90c6e8e129fa5a69f07982a4d9 https://oarep.usim.edu.my/bitstreams/5047f61a-2962-40e8-9ee3-38dd38698a1c/download cfe599990c2899f87d4f5037293e8e0d https://oarep.usim.edu.my/bitstreams/c556fbcd-3a48-4b59-9690-7acb89549123/download 277333f956e399513b0c2d178ccd23cd https://oarep.usim.edu.my/bitstreams/861fa131-1544-4c3e-9e3a-ac81178c6b3c/download cea2c56351223b40884c852c485427dd https://oarep.usim.edu.my/bitstreams/53fba818-7bb4-4369-a9a5-ef37b51c2e4f/download 6470e2961e17b6603f955027c25106b4 Biochemical Compounds and Principal Components Analysis(PCA) Dates (Fruit) -- Extraction (Chemistry) -- Analysis Dates (Fruit) -- Bioactive compounds BSTFA TMCS
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	Biochemical Compounds and Principal Components Analysis(PCA) Dates (Fruit) -- Extraction (Chemistry) -- Analysis Dates (Fruit) -- Bioactive compounds BSTFA TMCS
spellingShingle	Biochemical Compounds and Principal Components Analysis(PCA) Dates (Fruit) -- Extraction (Chemistry) -- Analysis Dates (Fruit) -- Bioactive compounds BSTFA TMCS Taffaha A. Arhouma Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis
description	In this study, the extraction using different solvents (chloroform, methanol and hexane) with reagent N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA) and 1% trimethyl- chlorosilane’ (TMCS) and their mixtures were used to extract biochemical compounds in three types of dates Kheseab (Tamar), Segae (Rutab) and Khalas (Tamar). The compounds were determined using Gas chromatography-mass spectrometry (GS-MS) and Liquid chromatography time-of-flight mass spectrometer (LC-TOF-MS). The results of GC-MS showed that the extraction using the mixture of methanol and chloroform (50% : 50%) extracted 16 compounds followed by methanol with hexane (50% : 50%) which extracted 15 compounds, and mixture of three solvents (33.3% : 33.3% : 33.3%)b14 compounds. While the other mixture of solvents, less number of compounds were extracted as compared with the previous mixtures. The results of LC- TOF-MS showed that the extraction using methanol and chloroform (50% : 50%) in Khalas (Tamar) was able to extract 27 compounds followed by Segae (Rutab) with 17 compounds while in Kheseab (Tamar) only 10 compounds were extracted. Principal Components Analysis (PCA) was performed on multivariate data of GS-MS results to study the relationship between compounds extracted with polarity of solvents mixture from the scores and loadings plots.
format	Thesis
author	Taffaha A. Arhouma
author_facet	Taffaha A. Arhouma
author_sort	Taffaha A. Arhouma
title	Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis
title_short	Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis
title_full	Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis
title_fullStr	Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis
title_full_unstemmed	Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis
title_sort	extraction of biochemical compounds in dates using three mixture design and identification by gc-ms and lc-tof-ms with principal components analysis
granting_institution	Universiti Sains Islam Malaysia
url	https://oarep.usim.edu.my/bitstreams/05f1f44f-d05a-4234-bc4e-549313943a45/download https://oarep.usim.edu.my/bitstreams/ff0dbf8a-7f0b-4bdc-8887-c66478db212f/download https://oarep.usim.edu.my/bitstreams/0aa3b158-1f35-4b74-b233-00d7f2f674db/download https://oarep.usim.edu.my/bitstreams/46b80bdd-2236-40b4-b5cb-69c644c85521/download https://oarep.usim.edu.my/bitstreams/30608ab2-daf6-4f63-9887-4ba1fc1ce425/download https://oarep.usim.edu.my/bitstreams/333e9d42-fd13-4a60-9d94-2ec9457a462e/download https://oarep.usim.edu.my/bitstreams/bf791a21-532d-4315-a640-dc1899733c84/download https://oarep.usim.edu.my/bitstreams/ef42ec5b-2101-4fb0-bd53-8dc42ee6a1ed/download
_version_	1812444647315734528

Extraction of Biochemical Compounds in Dates using Three Mixture Design and Identification by GC-MS and LC-TOF-MS with Principal Components Analysis

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