Anti-Quorum Sensing Activity Of Medicinal Plants
The arising of multidrug resistance bacteria have led to the screening of various local medicinal plants for their anti-quorum sensing activity. Anti-quorum sensing compounds have been considered as a novel means to fight against these multidrug resistance bacteria as these compounds do not impos...
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| Format: | Thesis |
| Language: | English |
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| Summary: | The arising of multidrug resistance bacteria have led to the screening of various local
medicinal plants for their anti-quorum sensing activity. Anti-quorum sensing
compounds have been considered as a novel means to fight against these multidrug
resistance bacteria as these compounds do not impose any selective pressure on the
bacteria. Parkia spaciosa, Centella asiatica and Piper nigrum are well-known plants
of tropical region with many reported medicinal importance. Therefore this study was
carried out to screen the plants for anti-quorum sensing activity using methanolic and
ethanolic extracts. A reporter organism in quorum sensing Chromobacterium
violaceum was used as biomonitor organism. Anti-quorum sensing activities of the
selected plants were tested on C. violaceum ATCC 12472 using agar well diffusion
assay. The results showed observable zones of inhibition for both extracts except for
the P. nigrum which showed no inhibition at the concentration of 800mg/ml of
methanolic extract. Furthermore, comparison analysis between anti-quorum activity
and antibacterial effects were carried out by comparing clear zone of inhibition
produced as a result of tetracycline action with the cloudy zone of inhibition produced
by the extract from the selected plants. However, ethanolic extract of P. spaciosa
showed antibacterial activity at a slightly higher concentration (300mg/ml). The
observed zones of inhibition were concentration dependent and minimum of QS
inhibition concentration were established for each plant extract. This result suggests
that the extract of the selected plants can be used as anti-virulence agents.
Keywords: Parkia spaciosa, Centella asiatica, Piper nigrum Quorum sensing, Antiquorum sensing. |
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