Anti-Quorum Sensing Activity Of Medicinal Plants
The arising of multidrug resistance bacteria have led to the screening of various local medicinal plants for their anti-quorum sensing activity. Anti-quorum sensing compounds have been considered as a novel means to fight against these multidrug resistance bacteria as these compounds do not impos...
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my-usim-ddms-126162024-05-29T04:26:54Z Anti-Quorum Sensing Activity Of Medicinal Plants Ibraheem Saheed Adebayo The arising of multidrug resistance bacteria have led to the screening of various local medicinal plants for their anti-quorum sensing activity. Anti-quorum sensing compounds have been considered as a novel means to fight against these multidrug resistance bacteria as these compounds do not impose any selective pressure on the bacteria. Parkia spaciosa, Centella asiatica and Piper nigrum are well-known plants of tropical region with many reported medicinal importance. Therefore this study was carried out to screen the plants for anti-quorum sensing activity using methanolic and ethanolic extracts. A reporter organism in quorum sensing Chromobacterium violaceum was used as biomonitor organism. Anti-quorum sensing activities of the selected plants were tested on C. violaceum ATCC 12472 using agar well diffusion assay. The results showed observable zones of inhibition for both extracts except for the P. nigrum which showed no inhibition at the concentration of 800mg/ml of methanolic extract. Furthermore, comparison analysis between anti-quorum activity and antibacterial effects were carried out by comparing clear zone of inhibition produced as a result of tetracycline action with the cloudy zone of inhibition produced by the extract from the selected plants. However, ethanolic extract of P. spaciosa showed antibacterial activity at a slightly higher concentration (300mg/ml). The observed zones of inhibition were concentration dependent and minimum of QS inhibition concentration were established for each plant extract. This result suggests that the extract of the selected plants can be used as anti-virulence agents. Keywords: Parkia spaciosa, Centella asiatica, Piper nigrum Quorum sensing, Antiquorum sensing. Universiti Sains Islam Malaysia 2014-06 Thesis en https://oarep.usim.edu.my/handle/123456789/12616 https://oarep.usim.edu.my/bitstreams/a245f159-50a8-4827-9f0c-6bfa66581c33/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/9b9738a5-0c24-4bce-acd9-4323b76c2755/download b0efa26c69a993f1ffd8303091ddaaee https://oarep.usim.edu.my/bitstreams/d655d9e1-df3e-45db-b3f7-9bc3d0769d6f/download 395cbb876fbf5aadfe39f4dfd9b01928 https://oarep.usim.edu.my/bitstreams/115110b0-690a-4f3a-94c4-afd3767fa5ba/download cf63bb692fc0309d3e9283c48023158c https://oarep.usim.edu.my/bitstreams/b1be2b43-23dc-46bb-be12-f792ecd0f2bf/download e4e0474f3dbeb66ad7fe8e37df8f2d3f https://oarep.usim.edu.my/bitstreams/5135760a-7d0a-42c1-8aa8-17161a9764fe/download 359b0c223a80d3a32c8fdadf99a2bd35 https://oarep.usim.edu.my/bitstreams/86552e31-6783-4849-bb60-f6af66ffdeef/download 17308b2b6873db46b72369731c3eda2f https://oarep.usim.edu.my/bitstreams/5ce6e06b-4fba-40a3-ac6f-0c62c70c6bbf/download 6fa59f09a6624bc7951d44796d6e199e https://oarep.usim.edu.my/bitstreams/8246f039-2a41-4860-8fe6-b25c7332703d/download cf68eb53bfce17d45bdea74e79fc27c8 https://oarep.usim.edu.my/bitstreams/e2097599-315c-4d57-947d-8b7089db5c64/download 07309e4f00c5a16d36533051d81ef513 Parkia spaciosa, Centella asiatica, Piper nigrum Quorum sensing, Antiquorum sensing. Medicinal plants -- Analysis -- Malaysia Quorum sensing (Microbiology) -- Analysis |
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Universiti Sains Islam Malaysia |
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English |
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Parkia spaciosa, Centella asiatica, Piper nigrum Quorum sensing, Antiquorum sensing. Medicinal plants -- Analysis -- Malaysia Quorum sensing (Microbiology) -- Analysis |
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Parkia spaciosa, Centella asiatica, Piper nigrum Quorum sensing, Antiquorum sensing. Medicinal plants -- Analysis -- Malaysia Quorum sensing (Microbiology) -- Analysis Ibraheem Saheed Adebayo Anti-Quorum Sensing Activity Of Medicinal Plants |
| description |
The arising of multidrug resistance bacteria have led to the screening of various local
medicinal plants for their anti-quorum sensing activity. Anti-quorum sensing
compounds have been considered as a novel means to fight against these multidrug
resistance bacteria as these compounds do not impose any selective pressure on the
bacteria. Parkia spaciosa, Centella asiatica and Piper nigrum are well-known plants
of tropical region with many reported medicinal importance. Therefore this study was
carried out to screen the plants for anti-quorum sensing activity using methanolic and
ethanolic extracts. A reporter organism in quorum sensing Chromobacterium
violaceum was used as biomonitor organism. Anti-quorum sensing activities of the
selected plants were tested on C. violaceum ATCC 12472 using agar well diffusion
assay. The results showed observable zones of inhibition for both extracts except for
the P. nigrum which showed no inhibition at the concentration of 800mg/ml of
methanolic extract. Furthermore, comparison analysis between anti-quorum activity
and antibacterial effects were carried out by comparing clear zone of inhibition
produced as a result of tetracycline action with the cloudy zone of inhibition produced
by the extract from the selected plants. However, ethanolic extract of P. spaciosa
showed antibacterial activity at a slightly higher concentration (300mg/ml). The
observed zones of inhibition were concentration dependent and minimum of QS
inhibition concentration were established for each plant extract. This result suggests
that the extract of the selected plants can be used as anti-virulence agents.
Keywords: Parkia spaciosa, Centella asiatica, Piper nigrum Quorum sensing, Antiquorum sensing. |
| format |
Thesis |
| author |
Ibraheem Saheed Adebayo |
| author_facet |
Ibraheem Saheed Adebayo |
| author_sort |
Ibraheem Saheed Adebayo |
| title |
Anti-Quorum Sensing Activity Of Medicinal Plants |
| title_short |
Anti-Quorum Sensing Activity Of Medicinal Plants |
| title_full |
Anti-Quorum Sensing Activity Of Medicinal Plants |
| title_fullStr |
Anti-Quorum Sensing Activity Of Medicinal Plants |
| title_full_unstemmed |
Anti-Quorum Sensing Activity Of Medicinal Plants |
| title_sort |
anti-quorum sensing activity of medicinal plants |
| granting_institution |
Universiti Sains Islam Malaysia |
| _version_ |
1812444799241814016 |