The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology
A novel and simple dual lipases system was developed in attaining high performance of ferulate esters by transesterification reaction between ethyl ferulate and olive oil. Detection and identification of reaction products were facilitated by Fourier Transform Infrared (FTIR). Enzyme screening reveal...
Saved in:
| id |
my-usim-ddms-12620 |
|---|---|
| record_format |
uketd_dc |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
| language |
English |
| topic |
Olive oil--Analysis Transesterification Olive oil -- Chemical elements |
| spellingShingle |
Olive oil--Analysis Transesterification Olive oil -- Chemical elements Nurul Jannah Abd Rahman The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology |
| description |
A novel and simple dual lipases system was developed in attaining high performance of ferulate esters by transesterification reaction between ethyl ferulate and olive oil. Detection and identification of reaction products were facilitated by Fourier Transform Infrared (FTIR). Enzyme screening revealed 1: 9 w/w of Novozym 435-Lipozyme RM IM to be the most efficient lipases ratio for the reaction synthesis. By varying one-parameter-at-a-time, 60 degree celcius of reaction temperature, 12 hrs of incubation time, 80 mg of lipase dosage and 1: 2 of ethyl ferulate: olive oil ratio were shown to be the optimal and suitable reaction conditions. Response surface methodology (RSM) based on four-factor-five-level central composite design (CCD) was further used to study the interactive effects among the parameters. The best fitting model was quadratic polynomial where incubation time exhibited most significant effect over the other three parameters. A high percent conversion of 94.03 % was achieved under the optimum conditions, which compared well with the maximum predicted value of 94.83 %. Biological study of ferulate esters was measured based on their antimicrobial properties against both Gram-positive and Gram-negative bacteria. Screening by agar diffusion test was employed as preliminary features followed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. The results proved that the ferulate esters posses moderate antibacterial inhibition against Gram-positive and Gram-negative bacteria. Their physicochemical properties were also well examined through sun protection factor (SPF) value, peroxidase value, saponification value and iodine value. |
| format |
Thesis |
| author |
Nurul Jannah Abd Rahman |
| author_facet |
Nurul Jannah Abd Rahman |
| author_sort |
Nurul Jannah Abd Rahman |
| title |
The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology |
| title_short |
The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology |
| title_full |
The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology |
| title_fullStr |
The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology |
| title_full_unstemmed |
The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology |
| title_sort |
use of new dual lipases system in the synthesis of ferulate esters: optimization by response surface methodology |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
https://oarep.usim.edu.my/bitstreams/4114d805-cfec-4b03-be9f-740735f1cd30/download https://oarep.usim.edu.my/bitstreams/b3adb5d8-0ef6-48b4-b46f-63a28f49e66d/download https://oarep.usim.edu.my/bitstreams/01bf5089-5ab3-4ba7-a38d-1e1ddf7e4d93/download https://oarep.usim.edu.my/bitstreams/a0efa0c7-8687-4c88-b51c-4767080b9e6a/download https://oarep.usim.edu.my/bitstreams/7246f40a-5091-48e8-bf24-19af20f6365c/download https://oarep.usim.edu.my/bitstreams/7a37f5c7-f9a2-44a3-b408-67cbb8ba583b/download https://oarep.usim.edu.my/bitstreams/e6d6f90b-9349-41be-8dc6-0d23fe775edf/download https://oarep.usim.edu.my/bitstreams/0da55d79-509f-4d32-823f-89f1d4345064/download https://oarep.usim.edu.my/bitstreams/2546800a-642c-4b13-b0ab-a16186009f85/download https://oarep.usim.edu.my/bitstreams/84713432-15da-4ea8-aec9-3c9981ed0ed0/download |
| _version_ |
1812444721454252032 |
| spelling |
my-usim-ddms-126202024-05-29T18:58:09Z The use of new Dual Lipases System in the synthesis of Ferulate Esters: optimization by response Surface Methodology Nurul Jannah Abd Rahman A novel and simple dual lipases system was developed in attaining high performance of ferulate esters by transesterification reaction between ethyl ferulate and olive oil. Detection and identification of reaction products were facilitated by Fourier Transform Infrared (FTIR). Enzyme screening revealed 1: 9 w/w of Novozym 435-Lipozyme RM IM to be the most efficient lipases ratio for the reaction synthesis. By varying one-parameter-at-a-time, 60 degree celcius of reaction temperature, 12 hrs of incubation time, 80 mg of lipase dosage and 1: 2 of ethyl ferulate: olive oil ratio were shown to be the optimal and suitable reaction conditions. Response surface methodology (RSM) based on four-factor-five-level central composite design (CCD) was further used to study the interactive effects among the parameters. The best fitting model was quadratic polynomial where incubation time exhibited most significant effect over the other three parameters. A high percent conversion of 94.03 % was achieved under the optimum conditions, which compared well with the maximum predicted value of 94.83 %. Biological study of ferulate esters was measured based on their antimicrobial properties against both Gram-positive and Gram-negative bacteria. Screening by agar diffusion test was employed as preliminary features followed by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. The results proved that the ferulate esters posses moderate antibacterial inhibition against Gram-positive and Gram-negative bacteria. Their physicochemical properties were also well examined through sun protection factor (SPF) value, peroxidase value, saponification value and iodine value. Universiti Sains Islam Malaysia 2015 Thesis en https://oarep.usim.edu.my/handle/123456789/12620 https://oarep.usim.edu.my/bitstreams/b5f47a5a-39c8-44b1-8ac8-7b2a102a5893/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/4114d805-cfec-4b03-be9f-740735f1cd30/download 101428b18f912d9240df0d3ff5d08dd1 https://oarep.usim.edu.my/bitstreams/b3adb5d8-0ef6-48b4-b46f-63a28f49e66d/download 16951a72b5778a3102c96fb4a59c5f79 https://oarep.usim.edu.my/bitstreams/01bf5089-5ab3-4ba7-a38d-1e1ddf7e4d93/download 7a21ea6c7c67cb7770bf3860d80b8122 https://oarep.usim.edu.my/bitstreams/a0efa0c7-8687-4c88-b51c-4767080b9e6a/download 0946fef135b275d738607729e338a5b2 https://oarep.usim.edu.my/bitstreams/7246f40a-5091-48e8-bf24-19af20f6365c/download 6a25796de9daae74cebbd915055fb59a https://oarep.usim.edu.my/bitstreams/7a37f5c7-f9a2-44a3-b408-67cbb8ba583b/download 6bda2a342d543404abf18c5441e55e44 https://oarep.usim.edu.my/bitstreams/e6d6f90b-9349-41be-8dc6-0d23fe775edf/download 531c8dba5d1f97659e20540e5db11e82 https://oarep.usim.edu.my/bitstreams/0da55d79-509f-4d32-823f-89f1d4345064/download 77353398164ccae8bfbd62447d19b767 https://oarep.usim.edu.my/bitstreams/2546800a-642c-4b13-b0ab-a16186009f85/download d507dfd13a82521e44f59444bd0c49a6 https://oarep.usim.edu.my/bitstreams/84713432-15da-4ea8-aec9-3c9981ed0ed0/download f34ade26930c733dc34bb76ad66eedcd https://oarep.usim.edu.my/bitstreams/87424426-3704-4bd3-83f6-8674b746cc52/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/3015cdac-b182-4c7b-a939-7ed57fdbe650/download d73c64075f3313b3ee4f29dc7e6bbf63 https://oarep.usim.edu.my/bitstreams/c780b23d-98bf-4e1b-9bfb-984fd5f8297e/download ff4c8ff01d544500ea4bfea43e6108c1 https://oarep.usim.edu.my/bitstreams/4a2cf7b8-45ff-402f-a77d-59f5e44c8531/download 792680ea1ef80bbcb0718ee7aa1e2a75 https://oarep.usim.edu.my/bitstreams/2047295c-ecc4-430a-ae25-0d95b245a0b3/download 7f5b903a193cc66524e06d8c0458e34a https://oarep.usim.edu.my/bitstreams/eced536d-926e-4ba6-8022-ff623477b689/download 48175cc56e52e020bf178616c0977374 https://oarep.usim.edu.my/bitstreams/128a1c4d-645b-4db2-919c-0debc0182716/download 24d68299ae13852334a122ea657bbaaa https://oarep.usim.edu.my/bitstreams/a977175c-4be9-42e2-9f7a-768edbd7d763/download 33f4f15a16a9843faf6a25d4f387b6fd https://oarep.usim.edu.my/bitstreams/19d4f22c-fb3e-4719-9e24-da5fa81781b4/download 2228e977ebea8966e27929f43e39cb67 https://oarep.usim.edu.my/bitstreams/61179c28-f69f-4d4c-a161-a2414ba9cbfb/download 48b012ab8bc20a1da2bfe3249367e5f4 Olive oil--Analysis Transesterification Olive oil -- Chemical elements |