Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin
Detection of human chorionic gonadotropin (HCG) is one of many prominent diagnostics methods in obstetrics, gynaecology and oncology. In this study, the combination of gold nanoparticles (AuNPs) functionalized with glutathione disulfide (GSSG) and microfluidic paper-based analytical device (μPAD) wa...
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my-usim-ddms-126272024-05-29T18:40:36Z Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin Muhammad Anas Yuzairi bin Mohd Yusri Detection of human chorionic gonadotropin (HCG) is one of many prominent diagnostics methods in obstetrics, gynaecology and oncology. In this study, the combination of gold nanoparticles (AuNPs) functionalized with glutathione disulfide (GSSG) and microfluidic paper-based analytical device (μPAD) was used to detect and assess its performance on detection of HCG in the aspect of linearity, sensitivity and selectivity. AuNPs used in this study was successfully synthesized and characterized with particle and hydrodynamic size ranges at 4.89 – 34.40 nm and 31.03 nm – 10.19 μm respectively and capped with citrate ion. GSSG-functionalized AuNPs was successfully derivatized as the AuNP-based sensor for HCG. However, due to lack of visibility on the colour changes, the sensor was unable to be applied on the μPAD effectively. Meanwhile the AuNP-based sensor shown a good sensory performance as it responded linearly to the changing concentration of HCG with R2 value of 0.9877. The sensor also shown high sensitivity compared to previous studies with limit of detection value of 0.1577 ppm. Yet, the sensor produced was not highly selective to HCG as interference study shown high interference from the impurities added. Universiti Sains Islam Malaysia 2021-05 Thesis en_US https://oarep.usim.edu.my/handle/123456789/12627 https://oarep.usim.edu.my/bitstreams/94d06531-d1de-4969-aba0-50c81e9080eb/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/2cc0f16a-4c77-4f73-9cbb-48cb064e8050/download 4735dcfab4b95232eed371f91eb8ed21 https://oarep.usim.edu.my/bitstreams/c569401f-9e3d-4bf7-95e1-f96f2e0d3bfc/download 960e015b8c0318554a8dc403bf3c7ea2 https://oarep.usim.edu.my/bitstreams/6bf2b564-7458-484d-af0b-5c2481bdaefe/download b4cb3fc4b4664119e798dd3230fa82d0 https://oarep.usim.edu.my/bitstreams/8391c816-6550-4ec8-a005-9f85c8933c58/download 1a9d931d3e47ba38991c933b3f09f09e https://oarep.usim.edu.my/bitstreams/2f5206d8-76bd-4d33-9118-bc52c2074f9f/download 8687cf38cfe9cf3a4328c688d3d87f56 https://oarep.usim.edu.my/bitstreams/5b51416e-30fd-4905-9c28-9ed27ec65860/download 794f029778e07a12028477c1450ebcda https://oarep.usim.edu.my/bitstreams/e5523a30-8481-466d-8535-7d499a959561/download 7614880922ecad76cc77076dbe8bf2f3 https://oarep.usim.edu.my/bitstreams/d527d320-1d42-4eb5-968f-408e9a51340b/download 3d7b041422b71f09cfc22dd4b10800bc https://oarep.usim.edu.my/bitstreams/336cc7d1-fecf-4494-8520-ac63af8f2080/download 91c7ab2ee247f7815c8c74228551d718 https://oarep.usim.edu.my/bitstreams/2e1f3e62-7b74-4c3b-b160-a58284669d42/download b48d22c7d25808795ded12157e9ebdb5 https://oarep.usim.edu.my/bitstreams/bd423a3b-c511-4d86-9c0e-8184d531a0a7/download 6a1ee76dfbf60843f5eb367944ed4bd1 https://oarep.usim.edu.my/bitstreams/c1e26d89-86d1-40bb-b488-fe20b9b19355/download 0e05709c76bebb3c3d9d61dbaa42540a https://oarep.usim.edu.my/bitstreams/a1544d6b-0248-4d23-92a4-3d67f0e2923c/download 6bfbe22ea0e1d855b07e1102e0fd57cf https://oarep.usim.edu.my/bitstreams/65200be0-f7d3-4f92-9f66-ab87d1219b0e/download b5b040bedd2e59467fc0d7fe7ad1fc3f https://oarep.usim.edu.my/bitstreams/d631884e-b471-4b81-a646-9f9a717bd093/download e6d318d20f8b61279c4acf690d4f5e31 https://oarep.usim.edu.my/bitstreams/3c461baa-760e-44c5-a4df-f0dab0042ca1/download afd01c9aa58c233f5898359fb1d84851 https://oarep.usim.edu.my/bitstreams/4f7d3fc5-2f4a-4d0b-893d-fbe82bb2aa50/download 408aece22c1af898000b31e23a836881 https://oarep.usim.edu.my/bitstreams/cd062606-62a5-4247-9375-51484cc4ea65/download f4937047c449e5b04e9743a6e0ae8af5 https://oarep.usim.edu.my/bitstreams/a42fea83-b4f7-4303-af49-85dee76695ac/download 5242922ffaf94853f9b98f258c2db680 https://oarep.usim.edu.my/bitstreams/1cdbcbfe-b1e6-418e-af58-2dec0556a83e/download de92d60cc8f0e1e7909fd0f717f7ac1a Human chorionic gonadotropin gold nanoparticles--effectively |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
| language |
en_US |
| topic |
Human chorionic gonadotropin gold nanoparticles--effectively |
| spellingShingle |
Human chorionic gonadotropin gold nanoparticles--effectively Muhammad Anas Yuzairi bin Mohd Yusri Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin |
| description |
Detection of human chorionic gonadotropin (HCG) is one of many prominent diagnostics methods in obstetrics, gynaecology and oncology. In this study, the combination of gold nanoparticles (AuNPs) functionalized with glutathione disulfide (GSSG) and microfluidic paper-based analytical device (μPAD) was used to detect and assess its performance on detection of HCG in the aspect of linearity, sensitivity and selectivity. AuNPs used in this study was successfully synthesized and characterized with particle and hydrodynamic size ranges at 4.89 – 34.40 nm and 31.03 nm – 10.19 μm respectively and capped with citrate ion. GSSG-functionalized AuNPs was successfully derivatized as the AuNP-based sensor for HCG. However, due to lack of visibility on the colour changes, the sensor was unable to be applied on the μPAD effectively. Meanwhile the AuNP-based sensor shown a good sensory performance as it responded linearly to the changing concentration of HCG with R2 value of 0.9877. The sensor also shown high sensitivity compared to previous studies with limit of detection value of 0.1577 ppm. Yet, the sensor produced was not highly selective to HCG as interference study shown high interference from the impurities added. |
| format |
Thesis |
| author |
Muhammad Anas Yuzairi bin Mohd Yusri |
| author_facet |
Muhammad Anas Yuzairi bin Mohd Yusri |
| author_sort |
Muhammad Anas Yuzairi bin Mohd Yusri |
| title |
Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin |
| title_short |
Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin |
| title_full |
Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin |
| title_fullStr |
Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin |
| title_full_unstemmed |
Fabrication Of Gold Nanoparticles As Paper-Based Microfluidic Device For Colorimetric Assay Of Human Chorionic Gonadotropin |
| title_sort |
fabrication of gold nanoparticles as paper-based microfluidic device for colorimetric assay of human chorionic gonadotropin |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
https://oarep.usim.edu.my/bitstreams/2cc0f16a-4c77-4f73-9cbb-48cb064e8050/download https://oarep.usim.edu.my/bitstreams/c569401f-9e3d-4bf7-95e1-f96f2e0d3bfc/download https://oarep.usim.edu.my/bitstreams/6bf2b564-7458-484d-af0b-5c2481bdaefe/download https://oarep.usim.edu.my/bitstreams/8391c816-6550-4ec8-a005-9f85c8933c58/download https://oarep.usim.edu.my/bitstreams/2f5206d8-76bd-4d33-9118-bc52c2074f9f/download https://oarep.usim.edu.my/bitstreams/5b51416e-30fd-4905-9c28-9ed27ec65860/download https://oarep.usim.edu.my/bitstreams/e5523a30-8481-466d-8535-7d499a959561/download https://oarep.usim.edu.my/bitstreams/d527d320-1d42-4eb5-968f-408e9a51340b/download https://oarep.usim.edu.my/bitstreams/336cc7d1-fecf-4494-8520-ac63af8f2080/download https://oarep.usim.edu.my/bitstreams/2e1f3e62-7b74-4c3b-b160-a58284669d42/download |
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