Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1
Bacillus subtilis is able to synthesize surfactin with excellent-active properties and biological activities.Since local isolates of B. subtilis are abundant and cheap,production and quantification of surfactin produced by local isolate of B. subtilis named MSH1 were studied through shake flasks fer...
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| Format: | Thesis |
| Language: | English |
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| Summary: | Bacillus subtilis is able to synthesize surfactin with excellent-active properties and biological activities.Since local isolates of B. subtilis are abundant and cheap,production and quantification of surfactin produced by local isolate of B. subtilis named MSH1 were studied through shake flasks fermantetion and high performance liquid chromatography (HPLC). The experimental work was conducted to develop method of HPLC technique for surfactin production,as well as to assess the ability of local isolate to produce surfactin using Cooper’s media in shake flasks fermentation under the condition of 150 rpm for 96 h at 30°C. In this study the concentration of surfactin produced by B. subtilis MSH1 was 760 mg/L. Investigation on the antifungal activities of surfactin against pathogenic fungi,such as: Aspergillus niger, Colletotrichum gloeosporioides, Candida albicans, Candida tropicalis, Candida parapsilosis and Candida krusei.Agar-well diffusion assay was done using to identify the antimicrobial susceptibility. The surfactin showed a high antifungal activity towards A. niger, C. gloeosporioides and C. albicans compared to C. tropicalis, C. parapsilosis and C. jrusei in agar well-diffusion assay. This study showed high concentrations of surfactin ability to bactericide (MFC) more than low concentrations.The surfactin indicated high antifungal activity withminimum inhibition concentration (MIC) towards A. niger,C. gloeosporioides and C.krusei. |
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