Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1

Bacillus subtilis is able to synthesize surfactin with excellent-active properties and biological activities.Since local isolates of B. subtilis are abundant and cheap,production and quantification of surfactin produced by local isolate of B. subtilis named MSH1 were studied through shake flasks fer...

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Main Author:	Taiba A. Moftah Othman
Format:	Thesis
Language:	English
Subjects:	tropicalis Colletotrichum gloeosporioides
Online Access:	https://oarep.usim.edu.my/bitstreams/e601db9b-1829-4413-aeed-a4aaae5ddb88/download https://oarep.usim.edu.my/bitstreams/b1d384fb-314f-4da4-a2c0-3f98e223c213/download https://oarep.usim.edu.my/bitstreams/0ae7e7dc-cabb-46d0-960b-7c83fda1f0d4/download https://oarep.usim.edu.my/bitstreams/04f397c3-513d-49ee-997b-3be184842623/download https://oarep.usim.edu.my/bitstreams/533c9cca-6eb7-4af3-b869-2982a4f48954/download https://oarep.usim.edu.my/bitstreams/9a5a9c64-8d12-40f9-8885-c3848544b87f/download https://oarep.usim.edu.my/bitstreams/04d08067-30d8-4196-b035-c2c80f855a1b/download
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spelling	my-usim-ddms-126882024-05-29T19:15:55Z Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1 Taiba A. Moftah Othman Bacillus subtilis is able to synthesize surfactin with excellent-active properties and biological activities.Since local isolates of B. subtilis are abundant and cheap,production and quantification of surfactin produced by local isolate of B. subtilis named MSH1 were studied through shake flasks fermantetion and high performance liquid chromatography (HPLC). The experimental work was conducted to develop method of HPLC technique for surfactin production,as well as to assess the ability of local isolate to produce surfactin using Cooper’s media in shake flasks fermentation under the condition of 150 rpm for 96 h at 30°C. In this study the concentration of surfactin produced by B. subtilis MSH1 was 760 mg/L. Investigation on the antifungal activities of surfactin against pathogenic fungi,such as: Aspergillus niger, Colletotrichum gloeosporioides, Candida albicans, Candida tropicalis, Candida parapsilosis and Candida krusei.Agar-well diffusion assay was done using to identify the antimicrobial susceptibility. The surfactin showed a high antifungal activity towards A. niger, C. gloeosporioides and C. albicans compared to C. tropicalis, C. parapsilosis and C. jrusei in agar well-diffusion assay. This study showed high concentrations of surfactin ability to bactericide (MFC) more than low concentrations.The surfactin indicated high antifungal activity withminimum inhibition concentration (MIC) towards A. niger,C. gloeosporioides and C.krusei. Universiti Sains Islam Malaysia 2014-11 Thesis en https://oarep.usim.edu.my/handle/123456789/12688 https://oarep.usim.edu.my/bitstreams/263c77ec-bff6-4591-9734-5a30e2b05a34/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/e601db9b-1829-4413-aeed-a4aaae5ddb88/download e1bd0e28eb20fe181caf1f2ba24aff4b https://oarep.usim.edu.my/bitstreams/b1d384fb-314f-4da4-a2c0-3f98e223c213/download 6672be5b62014e914564864145f040f3 https://oarep.usim.edu.my/bitstreams/0ae7e7dc-cabb-46d0-960b-7c83fda1f0d4/download 8ed6eb23f25c62109946a927076da3de https://oarep.usim.edu.my/bitstreams/04f397c3-513d-49ee-997b-3be184842623/download 33feabe323625a499fe76e30546c32a3 https://oarep.usim.edu.my/bitstreams/533c9cca-6eb7-4af3-b869-2982a4f48954/download 3e1627c56edca2b41b995bd268c10fe5 https://oarep.usim.edu.my/bitstreams/9a5a9c64-8d12-40f9-8885-c3848544b87f/download 2d164313ad80a06fa6e8be0e5325b8b9 https://oarep.usim.edu.my/bitstreams/04d08067-30d8-4196-b035-c2c80f855a1b/download be62e445bf2e0a4565846865e8ea1429 https://oarep.usim.edu.my/bitstreams/4653d557-1234-4167-8b09-9ba23315c095/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/cfd73a87-6404-493c-99c7-1cc150aebb9b/download 86cfec16447214feb3809a7a1cb60925 https://oarep.usim.edu.my/bitstreams/82be6c5e-4b89-4ce3-ac25-a31b79a6f21f/download cc9067c2ee470dc248b14b194209a34e https://oarep.usim.edu.my/bitstreams/fb959ef9-30bd-4583-9166-84ca8e5704fb/download 24d68299ae13852334a122ea657bbaaa https://oarep.usim.edu.my/bitstreams/dcd4b934-89a2-4e11-9359-fc736986e79c/download 6d93d3216dc4a7f5df47d4876fbec4d3 https://oarep.usim.edu.my/bitstreams/dc9897ca-1fdd-4f2e-a49e-9fa3301ce2c5/download b308b7fe5c1c2bbdc0cb686d451b84aa https://oarep.usim.edu.my/bitstreams/b11656f7-839b-4992-af52-2d68c5737d9a/download 33f4f15a16a9843faf6a25d4f387b6fd tropicalis Colletotrichum gloeosporioides
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	tropicalis Colletotrichum gloeosporioides
spellingShingle	tropicalis Colletotrichum gloeosporioides Taiba A. Moftah Othman Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1
description	Bacillus subtilis is able to synthesize surfactin with excellent-active properties and biological activities.Since local isolates of B. subtilis are abundant and cheap,production and quantification of surfactin produced by local isolate of B. subtilis named MSH1 were studied through shake flasks fermantetion and high performance liquid chromatography (HPLC). The experimental work was conducted to develop method of HPLC technique for surfactin production,as well as to assess the ability of local isolate to produce surfactin using Cooper’s media in shake flasks fermentation under the condition of 150 rpm for 96 h at 30°C. In this study the concentration of surfactin produced by B. subtilis MSH1 was 760 mg/L. Investigation on the antifungal activities of surfactin against pathogenic fungi,such as: Aspergillus niger, Colletotrichum gloeosporioides, Candida albicans, Candida tropicalis, Candida parapsilosis and Candida krusei.Agar-well diffusion assay was done using to identify the antimicrobial susceptibility. The surfactin showed a high antifungal activity towards A. niger, C. gloeosporioides and C. albicans compared to C. tropicalis, C. parapsilosis and C. jrusei in agar well-diffusion assay. This study showed high concentrations of surfactin ability to bactericide (MFC) more than low concentrations.The surfactin indicated high antifungal activity withminimum inhibition concentration (MIC) towards A. niger,C. gloeosporioides and C.krusei.
format	Thesis
author	Taiba A. Moftah Othman
author_facet	Taiba A. Moftah Othman
author_sort	Taiba A. Moftah Othman
title	Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1
title_short	Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1
title_full	Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1
title_fullStr	Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1
title_full_unstemmed	Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1
title_sort	antifungal activity of surfactin produced by bacillus subtilis msh1
granting_institution	Universiti Sains Islam Malaysia
url	https://oarep.usim.edu.my/bitstreams/e601db9b-1829-4413-aeed-a4aaae5ddb88/download https://oarep.usim.edu.my/bitstreams/b1d384fb-314f-4da4-a2c0-3f98e223c213/download https://oarep.usim.edu.my/bitstreams/0ae7e7dc-cabb-46d0-960b-7c83fda1f0d4/download https://oarep.usim.edu.my/bitstreams/04f397c3-513d-49ee-997b-3be184842623/download https://oarep.usim.edu.my/bitstreams/533c9cca-6eb7-4af3-b869-2982a4f48954/download https://oarep.usim.edu.my/bitstreams/9a5a9c64-8d12-40f9-8885-c3848544b87f/download https://oarep.usim.edu.my/bitstreams/04d08067-30d8-4196-b035-c2c80f855a1b/download
_version_	1812444767760416768

Antifungal Activity of Surfactin Produced by Bacillus subtilis MSH1

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