Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy
Candida rugosa lipase (CRL) immobilized into the carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) thin films via the entrapment method was studied. Characterization of the thin films which was done using Scanning electron microscope (SEM) coupled with Electron dispersive X-ray (EDX) element...
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Universiti Sains Islam Malaysia |
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Thin films Cellulose |
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Thin films Cellulose Siti Aiyshah Binti Md Razali Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy |
| description |
Candida rugosa lipase (CRL) immobilized into the carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) thin films via the entrapment method was studied. Characterization of the thin films which was done using Scanning electron microscope (SEM) coupled with Electron dispersive X-ray (EDX) elemental analyzer, X-ray diffractometer (XRD) and Fourier transform infrared (FTIR) spectrometer showed that entrapment of the biocatalyst had occurred within the thin films. Characterization of the immobilized CRL within the polymeric thin films using SEM showed a mass of globular patterns which confirmed the existence of CRL on the surfaces of the supports. The analysis on the elemental compositions of all thin films showed that carbon (C), oxygen (O) and sodium (Na) were major elements in the thin films, while thin films containing CRL showed the existence of potassium (K) in addition to the major elements mentioned above. In the analysis using XRD, all of thin films exhibited almost similar d-spacings of between 4.14418 to 4.65414 Å and 2θ values of between 19.054o to 21.424o. Similar peaks were also detected for all thin films in the analysis using FTIR spectrometer. The newly prepared biocatalyst thin films were then tested for their abilities in catalyzing the esterification of butyl oleate, in reactions which consisted of butanol and oleic acid. Thin films of CMC-CRL, PVA-CRL and PVA:CMC-CRL, showed 2-3 folds higher yields of butyl oleate compared to 9% yield of ester produced without CRL. The effect of storage stability at different temperatures (-20oC, 0oC, 4oC and room temperature) was also evaluated where the highest percent of conversion (28.9%) was obtained using PVA-CRL at storage temperature of -20oC. In the operational stability study, it was observed that the CMC-CRL, PVA-CRL and PVA:CMC-CRL were capable of retaining 50% of their catalytic activities even after 10 cycles of repeated uses. A kinetic study was also done to evaluate the efficacy of the free CRL and immobilized CRL. In the study, all immobilized CRL generally showed higher reaction rates than the free CRL. There were 2 types of conditions whereas at constant concentration of butanol and varying
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concentration of oleic acid and vice versa. PVA:CMC-CRL thin film showed the highest reaction rate compared to the free CRL . The immobilised lipases such as CMC-CRL, PVA-CRL and PVA:CMC –CRL have also showed considerably higher Vmax(Ol) compared to the native one in reactions of different concentration of oleic acid. The study of kinetics findings of one substrate (CMC-CRL, PVA-CRL and PVA:CMC-CRL) and two substrates (native-CRL) competitive were clearly explained by Bi Bi Ping Pong Model mechanism. These results indicated that the polymeric materials used for the entrapment of lipase in this study are potential supports for maintaining stability and activity of CRL for application in organic syntheses. |
| format |
Thesis |
| author |
Siti Aiyshah Binti Md Razali |
| author_facet |
Siti Aiyshah Binti Md Razali |
| author_sort |
Siti Aiyshah Binti Md Razali |
| title |
Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy |
| title_short |
Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy |
| title_full |
Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy |
| title_fullStr |
Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy |
| title_full_unstemmed |
Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy |
| title_sort |
evalution of thin films prepared from carboxymethyl cellulose (cmc) and polyvinyl alcohol (pva) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy |
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Universiti Sains Islam Malaysia |
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my-usim-ddms-126962024-06-11T18:01:25Z Evalution of thin films prepared from carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) entrapped with candida rugosa lipase for enhanced biocatalytic efficacy Siti Aiyshah Binti Md Razali Candida rugosa lipase (CRL) immobilized into the carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) thin films via the entrapment method was studied. Characterization of the thin films which was done using Scanning electron microscope (SEM) coupled with Electron dispersive X-ray (EDX) elemental analyzer, X-ray diffractometer (XRD) and Fourier transform infrared (FTIR) spectrometer showed that entrapment of the biocatalyst had occurred within the thin films. Characterization of the immobilized CRL within the polymeric thin films using SEM showed a mass of globular patterns which confirmed the existence of CRL on the surfaces of the supports. The analysis on the elemental compositions of all thin films showed that carbon (C), oxygen (O) and sodium (Na) were major elements in the thin films, while thin films containing CRL showed the existence of potassium (K) in addition to the major elements mentioned above. In the analysis using XRD, all of thin films exhibited almost similar d-spacings of between 4.14418 to 4.65414 Å and 2θ values of between 19.054o to 21.424o. Similar peaks were also detected for all thin films in the analysis using FTIR spectrometer. The newly prepared biocatalyst thin films were then tested for their abilities in catalyzing the esterification of butyl oleate, in reactions which consisted of butanol and oleic acid. Thin films of CMC-CRL, PVA-CRL and PVA:CMC-CRL, showed 2-3 folds higher yields of butyl oleate compared to 9% yield of ester produced without CRL. The effect of storage stability at different temperatures (-20oC, 0oC, 4oC and room temperature) was also evaluated where the highest percent of conversion (28.9%) was obtained using PVA-CRL at storage temperature of -20oC. In the operational stability study, it was observed that the CMC-CRL, PVA-CRL and PVA:CMC-CRL were capable of retaining 50% of their catalytic activities even after 10 cycles of repeated uses. A kinetic study was also done to evaluate the efficacy of the free CRL and immobilized CRL. In the study, all immobilized CRL generally showed higher reaction rates than the free CRL. There were 2 types of conditions whereas at constant concentration of butanol and varying viii concentration of oleic acid and vice versa. PVA:CMC-CRL thin film showed the highest reaction rate compared to the free CRL . The immobilised lipases such as CMC-CRL, PVA-CRL and PVA:CMC –CRL have also showed considerably higher Vmax(Ol) compared to the native one in reactions of different concentration of oleic acid. The study of kinetics findings of one substrate (CMC-CRL, PVA-CRL and PVA:CMC-CRL) and two substrates (native-CRL) competitive were clearly explained by Bi Bi Ping Pong Model mechanism. These results indicated that the polymeric materials used for the entrapment of lipase in this study are potential supports for maintaining stability and activity of CRL for application in organic syntheses. Universiti Sains Islam Malaysia 2020-12 Thesis en_US https://oarep.usim.edu.my/handle/123456789/12696 https://oarep.usim.edu.my/bitstreams/25589c16-e507-46d3-beaf-b1f18f71fe3b/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/2f1b1558-a5ad-4860-8817-9f01658c76bb/download b80d6359178dcfea2ca61ee769feb897 https://oarep.usim.edu.my/bitstreams/7734d3a7-38e2-4c1f-9de4-afdf50f75704/download 094e9ec80390fe9dc7d5e97617738a25 https://oarep.usim.edu.my/bitstreams/86d13be7-f718-4515-8a6c-c7d3e0997a56/download c8a1245473df1044f74fdcd7c4b7fb6c https://oarep.usim.edu.my/bitstreams/cc944a21-add3-4493-9d69-1be8004e399a/download 74e995e5386bd16f3f10bd9aced1ecb9 https://oarep.usim.edu.my/bitstreams/8a515354-a43d-4176-95f8-9b66a85ea40b/download 12d023b509e5ae620ff4506d0ffedcd2 https://oarep.usim.edu.my/bitstreams/ac86f68a-5364-44dc-87c1-e01045788a92/download 2c696fe0cd4356dab64a90b7fa865970 https://oarep.usim.edu.my/bitstreams/3c8dd286-58fa-4270-a4ed-b8e874bf4572/download 015de816f27a1a8dd86b85487906f878 https://oarep.usim.edu.my/bitstreams/704d1a52-1706-4263-94e2-27362d5348a7/download 16d8c2b85fb647aaac1733fb168dad8b https://oarep.usim.edu.my/bitstreams/c4971495-0f3d-47aa-ae4f-377e65e68981/download 3792b116e27f722d410e65705b0692ac https://oarep.usim.edu.my/bitstreams/dcc48f67-69bf-4b44-8745-c6fd65cf9c47/download 3cefb261829ae74b1216123225655942 https://oarep.usim.edu.my/bitstreams/cdb7640e-ee44-4072-9a9d-9355624d15ac/download 41737ca44d690e3ef0b2556e6d874480 https://oarep.usim.edu.my/bitstreams/9e7accca-e228-48fb-93ac-10ebcb547d8f/download b0ca0114d7c34fb29fb4e32b4036d293 https://oarep.usim.edu.my/bitstreams/2e269f98-20aa-4cc2-88fd-bb6c021d657d/download e52710168ed362aac4b06cb9f10a8d00 https://oarep.usim.edu.my/bitstreams/a0535d3c-f7af-4923-972c-91e5eab0ea0a/download d9727a7df05e35a12ff74858cb9ecf94 https://oarep.usim.edu.my/bitstreams/1c151d86-80cb-46e0-8c41-fd1af5ace79a/download ea03c1dffaba393a6c545b532532646a https://oarep.usim.edu.my/bitstreams/666d0d57-4e42-4753-8973-9dd028365994/download 2db0f7bc5b9795f4bc3f6306bdf47829 https://oarep.usim.edu.my/bitstreams/ebcf30b7-1e67-41fb-8857-02efa079c318/download 46ce582520fba2956108b0c57b17ce58 https://oarep.usim.edu.my/bitstreams/439078c5-411e-4948-a685-09bbbd5e6dec/download 8af347a2c710274d13b15c30d003fdb5 Thin films Cellulose |