Study On Antimicrobial Activity And Combination Effects Of Centella Asiatica
Antimicrobial activities of aqueous, methanol and ethanol extracts from Centella asiutica were evaluated against six strains of Gram positive and Gram negative bacteria. including Bacillus subtilis, Staphylococcus epidermis, Staphylococcus aureus, Escherichia coli. Salmonella typhimurium and Serr...
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| Format: | Thesis |
| Language: | English |
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| Summary: | Antimicrobial activities of aqueous, methanol and ethanol extracts from Centella asiutica
were evaluated against six strains of Gram positive and Gram negative bacteria. including
Bacillus subtilis, Staphylococcus epidermis, Staphylococcus aureus, Escherichia coli.
Salmonella typhimurium and Serratia marcescens by agar-well diffusion assay and
microdilution test. The agar-well diffussion assay was carried out to identify the
antimicrobial susceptibility. while the microdilution test was done to determine the
minimum inhibition concentration (MIC) and minimum bactericidal concentration
(MBC). Generally, the methanol, ethanol and aqueous extracts of C. asiatica showed
antimicrobial activity against all the bacteria tested. The aqueous and ethanol extracts
indicated high antimicrobial activity with MIC value of 62.5 mg/ml towards S.
typhimurium and E.coli. Whilst, the moderate antimicrobial activity with MIC value of
125 mg/ml showed by aqueous extract towards B. subtilis, S. aureus, S. epidermidis and
S. marcescens, methanol extract towards all six bacteria strains tested as well as ethanol
extract towards B. surbtilis, S. aureus, S. epidermidis, E. coli and S. marcescens. The
aqueous, methanol and ethanol extracts also exhibited a bactericidal effect towards five
strains of bacteria, including B. subtilis, S. aureus, E. coli, S. typhimurium and S.
marcescens. The MBCIMIC ratio of C. asiatica extracts was less than or equal to 4 (<4)
against the five tested bacteria indicated that the bacteria were sensitive towards C. asiatica extract.
In combination study, the aqueous and methanol extracts from C. asiatica
were combined with Streptomycin sulfate. The synergism effect was only
indicated for the combination treatments between each C. asiatica aqueous and methanol
extracts with Streptomycin sulfate towards B. subtilis. The alltagonisms effects could be
observed only in the combination treatments between C. asiatica aqueous extracts with
Streptomycins Sulfate against E. coli. However, the indifferent effects were showed by the
combination treatments between C. asiatica methanol extracts with Streptomycins sulfate
against E. coli. Generally, all the combination treatments on both bacteria indicated
additive and indifferent effects. The majority of the additive and synergism effects could
be found Gram positive bacteria (B. .subtilis). while antagonism effect occured on
Gram negative bacteria (E. coli). |
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