Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus

ABSTRACT Antimicrobial activities of aqueous, methanol and ethanol extracts from Polygonum minus were evaluated against six strains of Gram positive and Gram negative bacteria, including Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium an...

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Main Author:	Khadija Zedan Hassan
Format:	Thesis
Language:	English
Subjects:	Antimicrobial activities Polygonum minus Polygonaceae Analysis Microbiology
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spelling	my-usim-ddms-127372024-05-29T04:45:01Z Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus Khadija Zedan Hassan ABSTRACT Antimicrobial activities of aqueous, methanol and ethanol extracts from Polygonum minus were evaluated against six strains of Gram positive and Gram negative bacteria, including Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Serratiamarcescens by agar-well diffusion assay and microdilution test. The agar-well diffussion assay was carried out to identify the antimicrobial susceptibility, while the microdilution test was done to determine the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). Generally, P. minus ethanol, methanol and aqueous extracts showed a broad spectrum of antimicrobial activity on the tested bacteria. In agar-well diffusion test, the range of inhibition zone produced was between 6 mm to 13.5 mm in diameter. In microdilution assay, high inhibition effect with the lowest MIC value (62.50 mg/ml) showed by aqueous extracts towards S. typhimurium and B. subtilis, methanol extracts towards B. subtilis as well as ethanol extracts towards S. aureus, S. epidermidis and S. typhymurium. The aqueous and methanol extracts also exhibited a bactericidal effect towards all six strains of bacteria. However, ethanol extracts could only kill five bacterial strains, including B. subtilis, S. aureus, S. typhimurium, S. epidermidis and S. marcescens. In general, all test bacteria were susceptible to P. minus aqueous, methanol and ethanol extracts, except S. typhimurium whose MBC/MIC value was greater than 4 (>4) towards ethanol extracts. In combination study, the methanol and ethanol extracts from P. minus were combined with Streptomycin sulfate. The combination effects of P. minus methanol extracts with Streptomycin sulfate against B. subtilis showed that about 16.7% were synergy (two out of 12 combinations), 16.7% were additive (two out of 12 combinations) and 66.7% were indifferent (eight out of 12 combinations). Meanwhile, the combination effects between P. minus ethanol extract with Streptomycin sulfate against B. subtilis indicated that about 50% (six out of 12 combinations) were antagonistic, 16.67% (two out of 12 combinations) were indifferent and 33.33 % (four out of 12 combinations) were additive. Universiti Sains Islam Malaysia 2015-09 Thesis en https://oarep.usim.edu.my/handle/123456789/12737 https://oarep.usim.edu.my/bitstreams/8b8bc64c-0600-42b1-9b0d-8a8d4e02b9d2/download 8a4605be74aa9ea9d79846c1fba20a33 Antimicrobial activities Polygonum minus Polygonaceae Analysis Microbiology
institution	Universiti Sains Islam Malaysia
collection	USIM Institutional Repository
language	English
topic	Antimicrobial activities Polygonum minus Polygonaceae Analysis Microbiology
spellingShingle	Antimicrobial activities Polygonum minus Polygonaceae Analysis Microbiology Khadija Zedan Hassan Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus
description	ABSTRACT Antimicrobial activities of aqueous, methanol and ethanol extracts from Polygonum minus were evaluated against six strains of Gram positive and Gram negative bacteria, including Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Serratiamarcescens by agar-well diffusion assay and microdilution test. The agar-well diffussion assay was carried out to identify the antimicrobial susceptibility, while the microdilution test was done to determine the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). Generally, P. minus ethanol, methanol and aqueous extracts showed a broad spectrum of antimicrobial activity on the tested bacteria. In agar-well diffusion test, the range of inhibition zone produced was between 6 mm to 13.5 mm in diameter. In microdilution assay, high inhibition effect with the lowest MIC value (62.50 mg/ml) showed by aqueous extracts towards S. typhimurium and B. subtilis, methanol extracts towards B. subtilis as well as ethanol extracts towards S. aureus, S. epidermidis and S. typhymurium. The aqueous and methanol extracts also exhibited a bactericidal effect towards all six strains of bacteria. However, ethanol extracts could only kill five bacterial strains, including B. subtilis, S. aureus, S. typhimurium, S. epidermidis and S. marcescens. In general, all test bacteria were susceptible to P. minus aqueous, methanol and ethanol extracts, except S. typhimurium whose MBC/MIC value was greater than 4 (>4) towards ethanol extracts. In combination study, the methanol and ethanol extracts from P. minus were combined with Streptomycin sulfate. The combination effects of P. minus methanol extracts with Streptomycin sulfate against B. subtilis showed that about 16.7% were synergy (two out of 12 combinations), 16.7% were additive (two out of 12 combinations) and 66.7% were indifferent (eight out of 12 combinations). Meanwhile, the combination effects between P. minus ethanol extract with Streptomycin sulfate against B. subtilis indicated that about 50% (six out of 12 combinations) were antagonistic, 16.67% (two out of 12 combinations) were indifferent and 33.33 % (four out of 12 combinations) were additive.
format	Thesis
author	Khadija Zedan Hassan
author_facet	Khadija Zedan Hassan
author_sort	Khadija Zedan Hassan
title	Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus
title_short	Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus
title_full	Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus
title_fullStr	Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus
title_full_unstemmed	Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus
title_sort	study on antimicrobial activity and combination effects of polygonum minus
granting_institution	Universiti Sains Islam Malaysia
_version_	1812444652304859136

Study On Antimicrobial Activity And Combination Effects Of Polygonum Minus

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