Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants
Chilli plants are easily infected by Fusar-iu n species and cause enormous loss of food products as well as plants worldwide. Therefore, a suitable method is required for prevention and reduction of Fuscn-irun species affecting chilli plants. This research explored the possibility of using lactic...
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my-usim-ddms-12981 |
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uketd_dc |
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Universiti Sains Islam Malaysia |
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USIM Institutional Repository |
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English |
| topic |
Lactic acid bacteria Antifungal agents Chilli plants |
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Lactic acid bacteria Antifungal agents Chilli plants Akaram Husain Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants |
| description |
Chilli plants are easily infected by Fusar-iu n species and cause enormous loss of food
products as well as plants worldwide. Therefore, a suitable method is required for
prevention and reduction of Fuscn-irun species affecting chilli plants. This research
explored the possibility of using lactic acid bacteria (LAB) as bio-control against
Fusarium species on chilli plants. A total of 21 LABs were isolated from different
sources 14 from soil, 7 from fermented chilli fruits and 3 ATCC strains. Four
Fusarium species were isolated from different plants parts and identified as Fusar-icun
oxvsporum f. sp. lycopersici, F. solani, F. acwwninatunr and F. proliferation. Screening
using dual overlay assay showed that 14 from 21 LAB isolates inhibited F-us. ar-iurn
species with zone of inhibition 4.0 to 60.0 mm after 48 h incubation at 28°C. The
supernatants of Lb. acidophilus ATCC314, Lb. plantarrun ATCC8014 and three other
LAB isolates were identified as Lb. plantaruml-LAB MSS1 (isolated from soil),
Pediococcus pentosaceusl-LAB MSS5 (isolated from soil) and Lb. plantaruml-LAB
FF11 (isolated from fermented chilli fruits) using API Kit and 16rDNA genotypic
identification showed strong antifungal activity against all targeted Fusariwn species.
The supernatants of five LAB isolates showed mycelial growth inhibition against all
Fusariran species especially F. solcrni CS (isolated from chilli seeds). The antifungal
activity of the LAB supernatants was affected by enzyme treatment; the antifungal
activity was in the range of 7.44 to 86.83% depending on LAB and Fusariwn species
Pepsin reduced the antifungal activity of supernatants LAB-CFS MSS 1, IDLAB6 and
FF11 against F. oxysporwn 1. sp. lycopersici- CL. The antifungal activity was
significantly (P<0.05) reduced by pH of supernatant. Loss of antifungal activity of
LAB supernatant was observed at pH 6 to 9, reduced at pH 2 and 5, but maintained at
pH 3 and 4. Heating LAB-CFS at temperatures 80°C and 90°C for 30 min and 121 °C
for 15 ruin resulted in loss of antifungal activity in MSS5, IDLAB6 and FF11 against
F. acu, ninatum-FC, while other LAB maintained the antifungal activity (3.09 to
97.75%) after 72 h incubation at 30°C. Application of LAB-CFS to seed prior to
sowing enhanced seed germination by 97% but not for seeds infected with the fungi;
the germination rate was reduced to 50.00%. However, chilli seeds treated with
supernatant of LAB-FFI I and cells of LAB-MSSI significantly (P<0.05) increased
the germination rate to about 98% even when the seeds were sowed in soils infected
with the fungi. Significant shoot and root elongation was observed when the seeds
were treated with CFS of LAB-FF 11 before sowing with an average of 11.60±0.57 cm
after 16 day incubation in the dark. Plants treated with LAB MSS 1 (group II) showed
broadest width canopy of 80.83±10.51 cm, significantly (P<0.05) higher compared to
LAB FF1 I (group IV) and other groups. Inoculating F. solani-CS to soil resulted in
abnormal growth of chilli plants (Group VI) and plant height reached 143.67±0.41 cm
than plants treated with LAB cells and control plants. Fungi infected plants showed an
increase in dry weight of plants (77.32 g) and the water content was 77.03% which
was less than plants from others group after 65 days of transplanting. The productivity
(number of fruits/plant) was significantly (P<0.05) higher (56.33±06.11) in plants
treated with LAB FF 1I (group IV) compare to other groups. Growth of plants without
LAB or fungi was slow and productivity was 2% per plant. Plants receiving treatments
LAB MSS1 (groups II) LAB MSSI and F. solani (group III), LAB FF11 (group IV),
and LAB FF11 and F. solarri (group VI) produced chilli fruits which ripened and
turned to red within 90 d, and plant growth continued until more than 110 d. However,
plant infected with F. solani (Group VI) started to show plant death after 65 days.
Both Fusarium species and the Lb. plantarum (MSS1 and FF11) LAB were found to
be endophytic in nature. Treatment of seeds and soil with selected LAB either as cells
or supernatant resulted in rapid growth of plants in the presence of Fusarium species
Therefore, this study demonstrated that selected LAB either cells or their supernatants
could be used as bio-control against Fusarium species infecting chilli plants. Treating
the seeds or soil using LAB either cells or supernatants enhanced plant growth,
improved the productivity of chilli plants and also suppressed the growth of F. solani. |
| format |
Thesis |
| author |
Akaram Husain |
| author_facet |
Akaram Husain |
| author_sort |
Akaram Husain |
| title |
Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants |
| title_short |
Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants |
| title_full |
Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants |
| title_fullStr |
Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants |
| title_full_unstemmed |
Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants |
| title_sort |
lactic acid bacteria as biocontrol agent against pathogenic fusarium species on chilli plants |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
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my-usim-ddms-129812024-05-29T18:28:46Z Lactic Acid Bacteria As Biocontrol Agent Against Pathogenic Fusarium Species On Chilli Plants Akaram Husain Chilli plants are easily infected by Fusar-iu n species and cause enormous loss of food products as well as plants worldwide. Therefore, a suitable method is required for prevention and reduction of Fuscn-irun species affecting chilli plants. This research explored the possibility of using lactic acid bacteria (LAB) as bio-control against Fusarium species on chilli plants. A total of 21 LABs were isolated from different sources 14 from soil, 7 from fermented chilli fruits and 3 ATCC strains. Four Fusarium species were isolated from different plants parts and identified as Fusar-icun oxvsporum f. sp. lycopersici, F. solani, F. acwwninatunr and F. proliferation. Screening using dual overlay assay showed that 14 from 21 LAB isolates inhibited F-us. ar-iurn species with zone of inhibition 4.0 to 60.0 mm after 48 h incubation at 28°C. The supernatants of Lb. acidophilus ATCC314, Lb. plantarrun ATCC8014 and three other LAB isolates were identified as Lb. plantaruml-LAB MSS1 (isolated from soil), Pediococcus pentosaceusl-LAB MSS5 (isolated from soil) and Lb. plantaruml-LAB FF11 (isolated from fermented chilli fruits) using API Kit and 16rDNA genotypic identification showed strong antifungal activity against all targeted Fusariwn species. The supernatants of five LAB isolates showed mycelial growth inhibition against all Fusariran species especially F. solcrni CS (isolated from chilli seeds). The antifungal activity of the LAB supernatants was affected by enzyme treatment; the antifungal activity was in the range of 7.44 to 86.83% depending on LAB and Fusariwn species Pepsin reduced the antifungal activity of supernatants LAB-CFS MSS 1, IDLAB6 and FF11 against F. oxysporwn 1. sp. lycopersici- CL. The antifungal activity was significantly (P<0.05) reduced by pH of supernatant. Loss of antifungal activity of LAB supernatant was observed at pH 6 to 9, reduced at pH 2 and 5, but maintained at pH 3 and 4. Heating LAB-CFS at temperatures 80°C and 90°C for 30 min and 121 °C for 15 ruin resulted in loss of antifungal activity in MSS5, IDLAB6 and FF11 against F. acu, ninatum-FC, while other LAB maintained the antifungal activity (3.09 to 97.75%) after 72 h incubation at 30°C. Application of LAB-CFS to seed prior to sowing enhanced seed germination by 97% but not for seeds infected with the fungi; the germination rate was reduced to 50.00%. However, chilli seeds treated with supernatant of LAB-FFI I and cells of LAB-MSSI significantly (P<0.05) increased the germination rate to about 98% even when the seeds were sowed in soils infected with the fungi. Significant shoot and root elongation was observed when the seeds were treated with CFS of LAB-FF 11 before sowing with an average of 11.60±0.57 cm after 16 day incubation in the dark. Plants treated with LAB MSS 1 (group II) showed broadest width canopy of 80.83±10.51 cm, significantly (P<0.05) higher compared to LAB FF1 I (group IV) and other groups. Inoculating F. solani-CS to soil resulted in abnormal growth of chilli plants (Group VI) and plant height reached 143.67±0.41 cm than plants treated with LAB cells and control plants. Fungi infected plants showed an increase in dry weight of plants (77.32 g) and the water content was 77.03% which was less than plants from others group after 65 days of transplanting. The productivity (number of fruits/plant) was significantly (P<0.05) higher (56.33±06.11) in plants treated with LAB FF 1I (group IV) compare to other groups. Growth of plants without LAB or fungi was slow and productivity was 2% per plant. Plants receiving treatments LAB MSS1 (groups II) LAB MSSI and F. solani (group III), LAB FF11 (group IV), and LAB FF11 and F. solarri (group VI) produced chilli fruits which ripened and turned to red within 90 d, and plant growth continued until more than 110 d. However, plant infected with F. solani (Group VI) started to show plant death after 65 days. Both Fusarium species and the Lb. plantarum (MSS1 and FF11) LAB were found to be endophytic in nature. Treatment of seeds and soil with selected LAB either as cells or supernatant resulted in rapid growth of plants in the presence of Fusarium species Therefore, this study demonstrated that selected LAB either cells or their supernatants could be used as bio-control against Fusarium species infecting chilli plants. Treating the seeds or soil using LAB either cells or supernatants enhanced plant growth, improved the productivity of chilli plants and also suppressed the growth of F. solani. Universiti Sains Islam Malaysia 2016-03 Thesis en https://oarep.usim.edu.my/handle/123456789/12981 https://oarep.usim.edu.my/bitstreams/006bedd9-e04f-4c88-915e-cd60b940bf66/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/68c082a3-016d-48cd-ae24-46fe43da9bf0/download ad27f621f4410b899db5ea29d8970e86 https://oarep.usim.edu.my/bitstreams/b3f25fcf-2d86-4874-95a0-04925df33c26/download 2ae00d4f5e78e9ee3b7e1d6ecae93736 https://oarep.usim.edu.my/bitstreams/120b0c11-c525-4642-a3b8-479f55f301e9/download 87a4b629c2f7fa6da9799a34a0f3dcf1 https://oarep.usim.edu.my/bitstreams/5851972e-f3fc-4849-9c97-c9b84daf3557/download 3babb698c14550c39d424839f471b13a https://oarep.usim.edu.my/bitstreams/b145b895-a390-43ce-bc67-d8ede391d55f/download bc57adc1e6952f965969567bead10890 https://oarep.usim.edu.my/bitstreams/8f5fb216-1e47-49a6-947e-47b48fdfaaa7/download b2e062e07258d3a90f1d645b20b0b0b2 https://oarep.usim.edu.my/bitstreams/1a111121-f413-49ab-b11b-0b314ad5bf80/download d5c9f7283e2483216236c1692bb2ff0b https://oarep.usim.edu.my/bitstreams/1f19e67b-37e3-4305-a9f4-fe1a205e3437/download 743eef69f2057ad5d39c89fa3fa30bc4 https://oarep.usim.edu.my/bitstreams/4093f2a6-da63-4e63-9a05-ab7197e2f090/download 0d625f8731a99455d373edb66e0108ce https://oarep.usim.edu.my/bitstreams/e8ea912d-284e-4416-b20b-c7ddefc1c4a0/download 90c3a4eb20b359461bf661fe5f9f3bdd https://oarep.usim.edu.my/bitstreams/2c751527-16d2-4ca6-82b7-f72da1cd983d/download 53a7076ddbbfb28f4d56c9c62e48c2c8 https://oarep.usim.edu.my/bitstreams/2e06e198-8284-4574-ade7-c98d9f240b4a/download d6dcc2391f306c5dc2ce1885941f85a3 https://oarep.usim.edu.my/bitstreams/41d933d6-52ad-4506-963a-570d2a0aa0da/download 07ddf2fba9ce1059ca08165fd8be74a8 https://oarep.usim.edu.my/bitstreams/32833f70-3710-49f5-b362-fad757db7c6a/download 79cb2ed60ebe8165a50b848f54591284 https://oarep.usim.edu.my/bitstreams/6aa1ba3a-b375-4f37-ae41-9766b2fd7cd1/download 730883f6284ef79db887a9e9745be123 https://oarep.usim.edu.my/bitstreams/5cd42593-3aa1-4dcb-87cc-a265bc65640d/download 330b112b0a0d93f4da512dbdf4e00508 https://oarep.usim.edu.my/bitstreams/03912384-12e2-48a3-ae32-cdb1c073e61f/download 199f768c6e8837c421b13b414667caff https://oarep.usim.edu.my/bitstreams/46daa9c5-b605-466a-b3ca-82a3634700a3/download 883366dcb05a4af63500736429823f76 https://oarep.usim.edu.my/bitstreams/2e5600ce-ea44-4553-88f5-c486aaa1defe/download 75b1085dbcf321e69d4dfa5b0a8e1ac3 https://oarep.usim.edu.my/bitstreams/bed09e75-2302-4304-97a3-297074caff75/download 894d1f3ad8f5d29f3ce86f86f4f7cd79 https://oarep.usim.edu.my/bitstreams/17290482-871c-4f4c-a679-3a9345f0523d/download ed01131fda919e46b56566937acf415c https://oarep.usim.edu.my/bitstreams/a45c2ff7-65d0-4cae-9667-23969c513c49/download 7e1f786a713a3b025233f76d16b94006 Lactic acid bacteria Antifungal agents Chilli plants |