Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach
Dates flesh (Phoenix dactylifera L.) has been reported can give beneficial effects on human body. Therefore, a study on metabolites changes in human urine after consumption of Ajwa dates or kurma Nabi has been performed. Initially, chemical compounds in Ajwa date were extracted using three mixture d...
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my-usim-ddms-13060 |
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uketd_dc |
| institution |
Universiti Sains Islam Malaysia |
| collection |
USIM Institutional Repository |
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en_US |
| topic |
Dates (Fruit) Health aspects Dates,Ajwa |
| spellingShingle |
Dates (Fruit) Health aspects Dates,Ajwa Nurul Elma binti Sabri Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach |
| description |
Dates flesh (Phoenix dactylifera L.) has been reported can give beneficial effects on human body. Therefore, a study on metabolites changes in human urine after consumption of Ajwa dates or kurma Nabi has been performed. Initially, chemical compounds in Ajwa date were extracted using three mixture design and were identified using GCMS. The main compounds were found are 2, 3-dihydro-3, 5- dihydroxy-6-methyl-4H-pyran-4-one; β-sitosterol; caprylic acid and capric acid which have been reported having pharmacological functions as antioxidants, anticancer, antifungal and anti-inflammatory, respectively. Then, metabolomics study was conducted on 10 volunteers with no history of chronic disease. All volunteers were required to consume 7 Ajwa date flesh with control diets during the experiments (4 days). Urine samples were taken twice on Day 1 (7am and 7pm), four times on Day 2 (0h, 4h, 8h, 12h), twice on Day 3 (24h and 36h) and twice on Day 4 (48h and 60h) after consumption of Ajwa date. Metabolites changes in urine samples were studied using 1H-NMR, GCMS and LC-QToF-MS. Investigation on 1H–NMR data found that date fruits give beneficial effect on human body where the intensity of the stress biomarker metabolites (alanine; citrate and hippurate) were significantly decreased after the consumption of Ajwa dates, but were elevated back to previous level (0h) after 24 hours. The intensity of creatinine (renal function biomarker) was significantly eliminated in 4h, 8h and 12h after consuming Ajwa dates and significantly increased after 24 hours consuming Ajwa dates. Scores and Loadings plots (PCA) on 1H-NMR data has illustrated a complete 24h cycle of the effects of Ajwa dates intake, which indicates the effect of consuming Ajwa dates on human body is within 24 hours. Furthermore, urine sample (0h to 24h) from one of ten volunteers was selected for further analysis using LC-QToF-MS technique. The results revealed that some phytochemicals such as alkaloids; sterols; phenols and flavonoids in urine samples were changed after taken date fruits. Meanwhile GCMS data on acid, alkaline and neutral extraction on urine samples showed that fatty acids such as palmitic acid and stearic acid in urine increased after consumption of date fruits where the dates containing fatty acids. Further investigation on the urinary metabolites using NMR based and MS-based had confirmed the beneficial effect of Ajwa dates in human health. Findings in this study have confirmed that metabolomics is an effective tool that can be applied to evaluate the metabolism of chemical compounds for human health. |
| format |
Thesis |
| author |
Nurul Elma binti Sabri |
| author_facet |
Nurul Elma binti Sabri |
| author_sort |
Nurul Elma binti Sabri |
| title |
Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach |
| title_short |
Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach |
| title_full |
Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach |
| title_fullStr |
Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach |
| title_full_unstemmed |
Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach |
| title_sort |
evaluation of metabolites in human urine post consumption of phoenix dactylifera l. using multi-platform metabolomic approach |
| granting_institution |
Universiti Sains Islam Malaysia |
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https://oarep.usim.edu.my/bitstreams/b76aaa23-a18e-4544-9f19-b923da9d1ca0/download https://oarep.usim.edu.my/bitstreams/87d9f992-cb8c-4945-a848-1b942be95260/download https://oarep.usim.edu.my/bitstreams/24d14500-0a36-4fbc-97d8-f3fdb616b7d9/download https://oarep.usim.edu.my/bitstreams/b5b62c98-212d-470b-86b7-ac5799ad6062/download https://oarep.usim.edu.my/bitstreams/3ca03762-0b76-4c68-ac68-abd811940165/download https://oarep.usim.edu.my/bitstreams/37e1d1b7-b3e7-4a40-87f2-8997482fe45c/download https://oarep.usim.edu.my/bitstreams/fcd5c5a5-f1e9-4371-81fe-bf8b6439e1ed/download https://oarep.usim.edu.my/bitstreams/5b85b8a5-ba68-4947-aeaa-2d77bed6e156/download https://oarep.usim.edu.my/bitstreams/cd717fee-b3b0-4978-99aa-d6854f9c5239/download |
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my-usim-ddms-130602024-05-29T18:27:30Z Evaluation Of Metabolites In Human Urine Post Consumption Of Phoenix Dactylifera L. Using Multi-Platform Metabolomic Approach Nurul Elma binti Sabri Dates flesh (Phoenix dactylifera L.) has been reported can give beneficial effects on human body. Therefore, a study on metabolites changes in human urine after consumption of Ajwa dates or kurma Nabi has been performed. Initially, chemical compounds in Ajwa date were extracted using three mixture design and were identified using GCMS. The main compounds were found are 2, 3-dihydro-3, 5- dihydroxy-6-methyl-4H-pyran-4-one; β-sitosterol; caprylic acid and capric acid which have been reported having pharmacological functions as antioxidants, anticancer, antifungal and anti-inflammatory, respectively. Then, metabolomics study was conducted on 10 volunteers with no history of chronic disease. All volunteers were required to consume 7 Ajwa date flesh with control diets during the experiments (4 days). Urine samples were taken twice on Day 1 (7am and 7pm), four times on Day 2 (0h, 4h, 8h, 12h), twice on Day 3 (24h and 36h) and twice on Day 4 (48h and 60h) after consumption of Ajwa date. Metabolites changes in urine samples were studied using 1H-NMR, GCMS and LC-QToF-MS. Investigation on 1H–NMR data found that date fruits give beneficial effect on human body where the intensity of the stress biomarker metabolites (alanine; citrate and hippurate) were significantly decreased after the consumption of Ajwa dates, but were elevated back to previous level (0h) after 24 hours. The intensity of creatinine (renal function biomarker) was significantly eliminated in 4h, 8h and 12h after consuming Ajwa dates and significantly increased after 24 hours consuming Ajwa dates. Scores and Loadings plots (PCA) on 1H-NMR data has illustrated a complete 24h cycle of the effects of Ajwa dates intake, which indicates the effect of consuming Ajwa dates on human body is within 24 hours. Furthermore, urine sample (0h to 24h) from one of ten volunteers was selected for further analysis using LC-QToF-MS technique. The results revealed that some phytochemicals such as alkaloids; sterols; phenols and flavonoids in urine samples were changed after taken date fruits. Meanwhile GCMS data on acid, alkaline and neutral extraction on urine samples showed that fatty acids such as palmitic acid and stearic acid in urine increased after consumption of date fruits where the dates containing fatty acids. Further investigation on the urinary metabolites using NMR based and MS-based had confirmed the beneficial effect of Ajwa dates in human health. Findings in this study have confirmed that metabolomics is an effective tool that can be applied to evaluate the metabolism of chemical compounds for human health. Universiti Sains Islam Malaysia 2021-05 Thesis en_US https://oarep.usim.edu.my/handle/123456789/13060 https://oarep.usim.edu.my/bitstreams/9aa63aa6-78d8-4251-9b07-b6dfd1e24c88/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/b76aaa23-a18e-4544-9f19-b923da9d1ca0/download 790cf4f187d81b3b5ce89a91e1c9c1c9 https://oarep.usim.edu.my/bitstreams/87d9f992-cb8c-4945-a848-1b942be95260/download f2803fdd6368fe5e4ca6b91fd68476e4 https://oarep.usim.edu.my/bitstreams/24d14500-0a36-4fbc-97d8-f3fdb616b7d9/download 43ff1083cf4b688005702fe004dc165a https://oarep.usim.edu.my/bitstreams/b5b62c98-212d-470b-86b7-ac5799ad6062/download 6ae22d532a4a62be7939c4b6435f475c https://oarep.usim.edu.my/bitstreams/3ca03762-0b76-4c68-ac68-abd811940165/download 115d7dbdf6e6fd90bfe1bda2ac0ec511 https://oarep.usim.edu.my/bitstreams/37e1d1b7-b3e7-4a40-87f2-8997482fe45c/download d0abeb67aa6e4344732c0ec2a6ebe441 https://oarep.usim.edu.my/bitstreams/fcd5c5a5-f1e9-4371-81fe-bf8b6439e1ed/download f9ac27ffee3c6c155db834a0728a83ab https://oarep.usim.edu.my/bitstreams/5b85b8a5-ba68-4947-aeaa-2d77bed6e156/download 43761eec5c4a6d8d057787601c3e58ae https://oarep.usim.edu.my/bitstreams/cd717fee-b3b0-4978-99aa-d6854f9c5239/download e6e57a53eef49bfc9ee7b4d2e953b797 https://oarep.usim.edu.my/bitstreams/12fe6143-8ea3-4b04-a70e-b1b6e1565433/download 7284fe7f7e2a9e780e930968873720b9 https://oarep.usim.edu.my/bitstreams/3df502ba-42f2-48ce-a7da-2226734b0cce/download d887f519a6b4eb0c462d95f143015759 https://oarep.usim.edu.my/bitstreams/20ea9225-1978-47dd-ba7e-19614e5549c1/download caf1213c3195751736cf9e83a22299da https://oarep.usim.edu.my/bitstreams/f629fd99-a742-4f97-9d9c-47be6874454e/download e6c9260d8797eff8ee5ea21066320ee6 https://oarep.usim.edu.my/bitstreams/47e43fda-30a6-4be0-82a5-8c06c2024152/download 524600f206d2b9cfa98e3b5ffb62587d https://oarep.usim.edu.my/bitstreams/9022f080-0e9e-47c9-a061-e4fe2c80236e/download 8364d48a4d41758fae7c12b0153d5114 https://oarep.usim.edu.my/bitstreams/35163a22-1aaa-49f1-aead-73e066ca335d/download 53f7b603ed6146b7ac30b296259b124e https://oarep.usim.edu.my/bitstreams/9cea71df-0459-4906-a102-49f96ed9ba0b/download 37d20569ccee4d09ddcb50c3e110dd8f https://oarep.usim.edu.my/bitstreams/f450465a-0abd-4895-8502-fc8d3017ebe9/download 35dc15307a1af65a210897b49c036e5a Dates (Fruit); Health aspects Dates,Ajwa |