Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia
INTRODUCTION: Iron deficiency anaemia (IDA) is a global health problem. Significant increase was shown in haematological parameters after tested date palm and goat milk (GM) on IDA rats. Therefore, this study will focus on the assessment of iron bioavailability in dates and GM using Caco-2 cell l...
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my-usim-ddms-13392 |
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uketd_dc |
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Universiti Sains Islam Malaysia |
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USIM Institutional Repository |
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en_US |
| description |
INTRODUCTION: Iron deficiency anaemia (IDA) is a global health problem.
Significant increase was shown in haematological parameters after tested date palm
and goat milk (GM) on IDA rats. Therefore, this study will focus on the assessment of
iron bioavailability in dates and GM using Caco-2 cell lines and their effects on
haematological parameter and iron store in IDA subject. MATERIALS AND
METHODS: Ajwa date, Mariami date,GM and goat milk fed Ajwa seed (GMFA) were
prepared using in vitro digestion method. The caco-2 cells were harvested and used for
analysis, and a standard curve was plotted. A quantitative study was conducted among
57 female adults who having mild IDA and assigned with different feeding regime.
Blood samples was taken and analysed for full blood count and iron profile. Statistical
analysis was performed to compare pre- and post-treatment. RESULTS: The standard
curve showed a linear equation: Ferritin(ng)/protein(mg) = (16.519×bread Fe)+16.255,
R2=0.974. Using this equation, the bioavailability of iron from the mixture of Mariami
date and GMFA shows the highest (78.01±5.26%). It is followed by Mariami date+GM
(61.87±3.70%), Mariami date (49.08±2.54%), Ajwa date+GMFA (45.22±2.39%),
Ajwa date+GM (42.56±8.61%), Ajwa date (26.48±1.32%), GMFA (9.19±0.94%) and
GM (0.87±1.14%). Significant improvement in the haemopoietic activity was reported
after subjects were given with date palm andGM treatment. Date palm treatment shows
a significant increment in reticulocyte count, haemoglobin and packed cell volume,
while GM treatment shows a significant increment in reticulocyte count, red blood cell
vi
and haemoglobin. Subjects who consume both date palm and GM show significant
increments in reticulocyte count and haemoglobin. Furthermore, a significant
improvement in ferritin levels was also observed. DISCUSSION: The study found the
iron bioavailability of date palm treatment alone and GM treatment alone was lower
than a mixture of date palm and GM treatment. The inclusion of goat’s milk in the diet
was proven to increase iron bioavailability, improve haematological parameters and
restore iron content. GM is poor in iron content, but it contains a higher soluble protein
which favours iron absorption. Apparently, the inclusion of dates in the diet would
appear to increase haemoglobin as it is rich in iron and high in iron bioavailability as
compared to GM. CONCLUSION: Findings support the hypothesis that date palm and
GM is beneficial in IDA. Inclusion of date palm and GM is recommended in the diet.
It was also noted that goat milk does not interfere with iron absorption. |
| format |
Thesis |
| author |
Nurul Nadiah Binti Mad Zahir |
| spellingShingle |
Nurul Nadiah Binti Mad Zahir Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia |
| author_facet |
Nurul Nadiah Binti Mad Zahir |
| author_sort |
Nurul Nadiah Binti Mad Zahir |
| title |
Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia |
| title_short |
Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia |
| title_full |
Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia |
| title_fullStr |
Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia |
| title_full_unstemmed |
Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia |
| title_sort |
iron bioavailability of dates (phoenix dactylifera) and goat milk and their effects on iron deficiency anaemia |
| granting_institution |
Universiti Sains Islam Malaysia |
| url |
https://oarep.usim.edu.my/bitstreams/b68182e6-9ba6-4aab-a622-3a66828b348d/download https://oarep.usim.edu.my/bitstreams/18342abe-2df9-4dfe-bc78-95a0810440b7/download https://oarep.usim.edu.my/bitstreams/9ed3d00d-a8ff-4d3f-8070-23b650e0a15d/download https://oarep.usim.edu.my/bitstreams/aa18100e-3c7e-4dfa-a7f9-3f335e8cf874/download https://oarep.usim.edu.my/bitstreams/4d54d60f-b539-4467-adbe-fbb11a16d74d/download https://oarep.usim.edu.my/bitstreams/c61c91b4-b267-4644-8091-b9dbe1ee52c9/download https://oarep.usim.edu.my/bitstreams/28fddc5e-fd68-4eaa-a423-d93f2257d3ca/download https://oarep.usim.edu.my/bitstreams/ca5da381-6bd7-4d9f-acd3-a5ae1587f28f/download https://oarep.usim.edu.my/bitstreams/1e1f3ac5-1726-418e-89fb-e9fc3a0a0a27/download |
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my-usim-ddms-133922024-05-29T18:30:31Z Iron Bioavailability of Dates (Phoenix Dactylifera) and Goat Milk and Their Effects on Iron Deficiency Anaemia Nurul Nadiah Binti Mad Zahir INTRODUCTION: Iron deficiency anaemia (IDA) is a global health problem. Significant increase was shown in haematological parameters after tested date palm and goat milk (GM) on IDA rats. Therefore, this study will focus on the assessment of iron bioavailability in dates and GM using Caco-2 cell lines and their effects on haematological parameter and iron store in IDA subject. MATERIALS AND METHODS: Ajwa date, Mariami date,GM and goat milk fed Ajwa seed (GMFA) were prepared using in vitro digestion method. The caco-2 cells were harvested and used for analysis, and a standard curve was plotted. A quantitative study was conducted among 57 female adults who having mild IDA and assigned with different feeding regime. Blood samples was taken and analysed for full blood count and iron profile. Statistical analysis was performed to compare pre- and post-treatment. RESULTS: The standard curve showed a linear equation: Ferritin(ng)/protein(mg) = (16.519×bread Fe)+16.255, R2=0.974. Using this equation, the bioavailability of iron from the mixture of Mariami date and GMFA shows the highest (78.01±5.26%). It is followed by Mariami date+GM (61.87±3.70%), Mariami date (49.08±2.54%), Ajwa date+GMFA (45.22±2.39%), Ajwa date+GM (42.56±8.61%), Ajwa date (26.48±1.32%), GMFA (9.19±0.94%) and GM (0.87±1.14%). Significant improvement in the haemopoietic activity was reported after subjects were given with date palm andGM treatment. Date palm treatment shows a significant increment in reticulocyte count, haemoglobin and packed cell volume, while GM treatment shows a significant increment in reticulocyte count, red blood cell vi and haemoglobin. Subjects who consume both date palm and GM show significant increments in reticulocyte count and haemoglobin. Furthermore, a significant improvement in ferritin levels was also observed. DISCUSSION: The study found the iron bioavailability of date palm treatment alone and GM treatment alone was lower than a mixture of date palm and GM treatment. The inclusion of goat’s milk in the diet was proven to increase iron bioavailability, improve haematological parameters and restore iron content. GM is poor in iron content, but it contains a higher soluble protein which favours iron absorption. Apparently, the inclusion of dates in the diet would appear to increase haemoglobin as it is rich in iron and high in iron bioavailability as compared to GM. CONCLUSION: Findings support the hypothesis that date palm and GM is beneficial in IDA. Inclusion of date palm and GM is recommended in the diet. It was also noted that goat milk does not interfere with iron absorption. Universiti Sains Islam Malaysia 2023-08 Thesis en_US https://oarep.usim.edu.my/handle/123456789/13392 https://oarep.usim.edu.my/bitstreams/b68182e6-9ba6-4aab-a622-3a66828b348d/download 022817880b1e24b14396eda6424587a5 https://oarep.usim.edu.my/bitstreams/18342abe-2df9-4dfe-bc78-95a0810440b7/download ec3b0a8d2ef1af27267f62e85f08427d https://oarep.usim.edu.my/bitstreams/9ed3d00d-a8ff-4d3f-8070-23b650e0a15d/download 856547b9ecad6bbb7b5e6f097b76bab3 https://oarep.usim.edu.my/bitstreams/aa18100e-3c7e-4dfa-a7f9-3f335e8cf874/download 605fa1c785813cf950701a6559de20a5 https://oarep.usim.edu.my/bitstreams/4d54d60f-b539-4467-adbe-fbb11a16d74d/download 679310adfd4b9e19a7f722bfd397e3e3 https://oarep.usim.edu.my/bitstreams/c61c91b4-b267-4644-8091-b9dbe1ee52c9/download 87e4eae10b31e8ca848db3d8ad1eea13 https://oarep.usim.edu.my/bitstreams/28fddc5e-fd68-4eaa-a423-d93f2257d3ca/download 910367b2d5979caf911a41629d8347c6 https://oarep.usim.edu.my/bitstreams/ca5da381-6bd7-4d9f-acd3-a5ae1587f28f/download 01e0bb11f0241b51bf1d28dfa4482330 https://oarep.usim.edu.my/bitstreams/1e1f3ac5-1726-418e-89fb-e9fc3a0a0a27/download 40653189a80b0f282d5fac24a1336e49 https://oarep.usim.edu.my/bitstreams/93a6f623-c598-45ad-b348-f93e8beefc76/download 8a4605be74aa9ea9d79846c1fba20a33 https://oarep.usim.edu.my/bitstreams/2e2afca9-76d5-41aa-96c2-1751815ea722/download 68b329da9893e34099c7d8ad5cb9c940 https://oarep.usim.edu.my/bitstreams/07fbc30d-f639-4f40-b2e2-b1b0b60773a0/download 7fd5e21d67a711026f00f09c6aa62ff0 https://oarep.usim.edu.my/bitstreams/b20c3790-e866-41c0-8605-010ee4b42dc2/download 2978ae5c3f4b1163c36eac3ad94290a1 https://oarep.usim.edu.my/bitstreams/ed3fe5d6-d58b-4940-a893-b62eba6513d4/download 87297bfad4f3d7f504c365c9fd942b5b https://oarep.usim.edu.my/bitstreams/4b7129b6-1cb9-4071-8fbd-4e0bb98cd6a4/download 061261e57422264a0972f95420c32edf https://oarep.usim.edu.my/bitstreams/a91bbfcf-0c9f-44e7-9e7c-055ea17d5050/download 3e821d4aa444d51dc473f89617dfc400 https://oarep.usim.edu.my/bitstreams/59c377ca-2ed9-4275-bb9c-451e31d74d92/download fa2843cf08cc8118ea22b7fbbb22d675 https://oarep.usim.edu.my/bitstreams/f9edf5ef-1481-419e-bbe2-b465d0e311b8/download 6b4891a3c7bb4ad0b3f30a20059decfe https://oarep.usim.edu.my/bitstreams/105b1434-07b6-4efb-98dd-311e95a369a8/download dfba804b595fe0c89072bda92b71c112 |