Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis second stage larvae into PCR2.1 TOPO vector....
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://eprints.usm.my/28927/1/Cloning%2C_expression_and_purification_of_Toxocara_canis_Recombinant_Antigens_%28rTES-26%2C_rTES-32%2C_rTES-120%29_and_development_of_serodiagnostic_test_for_toxocariasis.pdf |
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| Summary: | In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and
rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes
via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis
second stage larvae into PCR2.1 TOPO vector. Dalam kajian ini, DNA rekombinan T. canis yang mengkodkan antigen rekombinan
rTES-26, rTES-32 dan rTES-120 dihasilkan dengan mengklonkan kerangka bacaan terbuka
gen masing-masing melalui kaedah “reverse-transcriptase-PCR” (RT-PCR) menggunakan
mRNA yang diekstrak daripada kultur larva T. canis peringkat kedua ke dalam vektor
PCR2.1 TOPO. |
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