Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis

Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis

In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis second stage larvae into PCR2.1 TOPO vector....

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Main Author: Mohamad, Suharni
Format: Thesis
Language:English
Published: 2009
Subjects:
R5-130.5 General works
Online Access:http://eprints.usm.my/28927/1/Cloning%2C_expression_and_purification_of_Toxocara_canis_Recombinant_Antigens_%28rTES-26%2C_rTES-32%2C_rTES-120%29_and_development_of_serodiagnostic_test_for_toxocariasis.pdf
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spelling my-usm-ep.289272017-05-15T04:43:29Z Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis 2009 Mohamad, Suharni R5-130.5 General works In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis second stage larvae into PCR2.1 TOPO vector. Dalam kajian ini, DNA rekombinan T. canis yang mengkodkan antigen rekombinan rTES-26, rTES-32 dan rTES-120 dihasilkan dengan mengklonkan kerangka bacaan terbuka gen masing-masing melalui kaedah “reverse-transcriptase-PCR” (RT-PCR) menggunakan mRNA yang diekstrak daripada kultur larva T. canis peringkat kedua ke dalam vektor PCR2.1 TOPO. 2009 Thesis http://eprints.usm.my/28927/ http://eprints.usm.my/28927/1/Cloning%2C_expression_and_purification_of_Toxocara_canis_Recombinant_Antigens_%28rTES-26%2C_rTES-32%2C_rTES-120%29_and_development_of_serodiagnostic_test_for_toxocariasis.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-130.5 General works
spellingShingle R5-130.5 General works
Mohamad, Suharni
Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
description In this study, recombinant T. canis DNA which encode for rTES-26, rTES-32 and rTES-120 were produced by cloning of open-reading frames (ORF) of the respective genes via reverse-transcriptase-PCR (RT-PCR) using mRNA extracted from a culture of T. canis second stage larvae into PCR2.1 TOPO vector. Dalam kajian ini, DNA rekombinan T. canis yang mengkodkan antigen rekombinan rTES-26, rTES-32 dan rTES-120 dihasilkan dengan mengklonkan kerangka bacaan terbuka gen masing-masing melalui kaedah “reverse-transcriptase-PCR” (RT-PCR) menggunakan mRNA yang diekstrak daripada kultur larva T. canis peringkat kedua ke dalam vektor PCR2.1 TOPO.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Mohamad, Suharni
author_facet Mohamad, Suharni
author_sort Mohamad, Suharni
title Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
title_short Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
title_full Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
title_fullStr Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
title_full_unstemmed Cloning, Expression And Purification Of Toxocara Canis Recombinant Antigens (Rtes-26, Rtes-32, Rtes-120) And Development Of Serodiagnostic Test For Toxocariasis
title_sort cloning, expression and purification of toxocara canis recombinant antigens (rtes-26, rtes-32, rtes-120) and development of serodiagnostic test for toxocariasis
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Perubatan
publishDate 2009
url http://eprints.usm.my/28927/1/Cloning%2C_expression_and_purification_of_Toxocara_canis_Recombinant_Antigens_%28rTES-26%2C_rTES-32%2C_rTES-120%29_and_development_of_serodiagnostic_test_for_toxocariasis.pdf
_version_ 1747820043539841024

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