Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization

Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization

Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage...

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Main Author: Ho, Sing Yee
Format: Thesis
Language:English
Published: 2014
Subjects:
G Geography (General)
SF Animal culture
Online Access:http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf
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id my-usm-ep.29097
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spelling my-usm-ep.290972019-04-12T05:26:04Z Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization 2014 Ho, Sing Yee G Geography (General) SF Animal culture Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage embryos. Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa panjang daripada embrio peringkat blastula ikan zebra. 2014 Thesis http://eprints.usm.my/29097/ http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Kajian Samudera dan Pantai
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic G Geography (General)
SF Animal culture
spellingShingle G Geography (General)
SF Animal culture
Ho, Sing Yee
Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
description Feeder layers are commonly used in zebrafish embryonic stem (ES) cell cultures to enable proliferation and prevent differentiation of the ES cells. In this study, a culture method using feeder-free was employed to derive and establish longterm zebrafish ES cell line from zebrafish blastula stage embryos. Lapisan sel penyuap telah biasa digunakan dalam kultur sel tunjang embrionik daripada ikan zebra untuk membolehkan proliferasi dan mencegah pembezaan sel-sel tersebut. Dalam kajian ini, kaedah kultur tanpa lapisan sel penyuap telah diguna untuk menghasilkan titisan sel tunjang embrionik jangka masa panjang daripada embrio peringkat blastula ikan zebra.
format Thesis
qualification_level Master's degree
author Ho, Sing Yee
author_facet Ho, Sing Yee
author_sort Ho, Sing Yee
title Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_short Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_full Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_fullStr Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_full_unstemmed Establishment Of A Zebrafish Embryonic Stem Cell Line Using A Feeder-Free Culture Method And In Vitro Pluripotency Characterization
title_sort establishment of a zebrafish embryonic stem cell line using a feeder-free culture method and in vitro pluripotency characterization
granting_institution Universiti Sains Malaysia
granting_department Pusat Kajian Samudera dan Pantai
publishDate 2014
url http://eprints.usm.my/29097/1/Establishment_of_a_zebrafish_embryonic_stem_cell_line_using_a_feeder-free_culture_method_and_in_vitro_puripotency_characterization.pdf
_version_ 1747820081662918656

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