Experimental Rnomics: Towards The Identification And Characterization Of Non-Protein-Coding Ribonucleic Acids In Pathogenic Agent, Salmonella Typhi
RNA bukan-pengkod-protein (npcRNA) merupakan satu kelas pengawalatur-ribo yang bertindak di dalam bentuk kompleks RNA-protein (sebagai RNPs) didalam pelbagai laluan pengawalaturan. Tesis ini memberikan tumpuan ke atas_ pengenalpastian npcRNA secara eksperimental daripada bakteria patogenik Salmon...
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my-usm-ep.311482017-05-15T04:43:31Z Experimental Rnomics: Towards The Identification And Characterization Of Non-Protein-Coding Ribonucleic Acids In Pathogenic Agent, Salmonella Typhi 2008-06 Zakaria, Robaiza R735-854 Medical education. Medical schools. Research RNA bukan-pengkod-protein (npcRNA) merupakan satu kelas pengawalatur-ribo yang bertindak di dalam bentuk kompleks RNA-protein (sebagai RNPs) didalam pelbagai laluan pengawalaturan. Tesis ini memberikan tumpuan ke atas_ pengenalpastian npcRNA secara eksperimental daripada bakteria patogenik Salmonella enterica serovar Typhi (S. Typhi), penyebab penyakit demam kepialu. Melalui pendekatan RNomiks Eksperimental, 82 calon novel npcRNAdaripada perpustakaan cDNA S. Typhi telah dikenalpasti dan dicirikan. Daripada jumlah ini, 28 telah ditranskrip daripada IGR, 29 ditranskrip di dalam arah antisense kepada ORF dan 18 dikenalpasti bertindihan dengan ORF. Sementara 7 calon yang lain telah ditranskIlp daripada kawasan repititif dan beberapa kedudukan bukan repitatif yang lain. Sebelas npcRNA merupakan npcRNAs yang telahpun dilaporkan. Non-protein-coding RNA (npcRNA) is a large class of riboregulators that act in complex with proteins (as RNPs) in diverse regulatory pathways. This thesis focused on the experimental identification of small npcRNAs from Salmonella enterica serovar Typhi (s. Typhi), the aetiological agent of typhoid fever. By an Experimental RNomics approach, 82 species of uncharacterized novel npcRNA candidates were identified from library generated from different growth phases of a clinically isolated S. Typhi. From this, 28 were transcribed from the IGRs, 29 were transcribed in the antisense orientation of the ORFs and 18 were identified to overlap the ORFs. Another 7 candidates were transcribed from repetitive regions and several non-repetitive locations. Eleven known npcRNAs were also detected. 2008-06 Thesis http://eprints.usm.my/31148/ http://eprints.usm.my/31148/1/ROBAIZA_BIN_ZAKARIA.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Perubatan |
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Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
R735-854 Medical education Medical schools Research |
| spellingShingle |
R735-854 Medical education Medical schools Research Zakaria, Robaiza Experimental Rnomics: Towards The Identification And Characterization Of Non-Protein-Coding Ribonucleic Acids In Pathogenic Agent, Salmonella Typhi |
| description |
RNA bukan-pengkod-protein (npcRNA) merupakan satu kelas pengawalatur-ribo
yang bertindak di dalam bentuk kompleks RNA-protein (sebagai RNPs) didalam
pelbagai laluan pengawalaturan. Tesis ini memberikan tumpuan ke atas_ pengenalpastian
npcRNA secara eksperimental daripada bakteria patogenik Salmonella enterica serovar
Typhi (S. Typhi), penyebab penyakit demam kepialu. Melalui pendekatan RNomiks
Eksperimental, 82 calon novel npcRNAdaripada perpustakaan cDNA S. Typhi telah
dikenalpasti dan dicirikan. Daripada jumlah ini, 28 telah ditranskrip daripada IGR, 29
ditranskrip di dalam arah antisense kepada ORF dan 18 dikenalpasti bertindihan dengan
ORF. Sementara 7 calon yang lain telah ditranskIlp daripada kawasan repititif dan
beberapa kedudukan bukan repitatif yang lain. Sebelas npcRNA merupakan npcRNAs
yang telahpun dilaporkan.
Non-protein-coding RNA (npcRNA) is a large class of riboregulators that act in
complex with proteins (as RNPs) in diverse regulatory pathways. This thesis focused on
the experimental identification of small npcRNAs from Salmonella enterica serovar
Typhi (s. Typhi), the aetiological agent of typhoid fever. By an Experimental RNomics
approach, 82 species of uncharacterized novel npcRNA candidates were identified from
library generated from different growth phases of a clinically isolated S. Typhi. From
this, 28 were transcribed from the IGRs, 29 were transcribed in the antisense orientation
of the ORFs and 18 were identified to overlap the ORFs. Another 7 candidates were
transcribed from repetitive regions and several non-repetitive locations. Eleven known
npcRNAs were also detected. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Zakaria, Robaiza |
| author_facet |
Zakaria, Robaiza |
| author_sort |
Zakaria, Robaiza |
| title |
Experimental Rnomics: Towards The
Identification And Characterization Of
Non-Protein-Coding Ribonucleic Acids In
Pathogenic Agent, Salmonella Typhi
|
| title_short |
Experimental Rnomics: Towards The
Identification And Characterization Of
Non-Protein-Coding Ribonucleic Acids In
Pathogenic Agent, Salmonella Typhi
|
| title_full |
Experimental Rnomics: Towards The
Identification And Characterization Of
Non-Protein-Coding Ribonucleic Acids In
Pathogenic Agent, Salmonella Typhi
|
| title_fullStr |
Experimental Rnomics: Towards The
Identification And Characterization Of
Non-Protein-Coding Ribonucleic Acids In
Pathogenic Agent, Salmonella Typhi
|
| title_full_unstemmed |
Experimental Rnomics: Towards The
Identification And Characterization Of
Non-Protein-Coding Ribonucleic Acids In
Pathogenic Agent, Salmonella Typhi
|
| title_sort |
experimental rnomics: towards the
identification and characterization of
non-protein-coding ribonucleic acids in
pathogenic agent, salmonella typhi |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Perubatan |
| publishDate |
2008 |
| url |
http://eprints.usm.my/31148/1/ROBAIZA_BIN_ZAKARIA.pdf |
| _version_ |
1747820398257373184 |