Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes

Komuniti mikrob bagi dua tanah paya bakau Pulau Pinang (Batu Maung dan Balik Pulau) yang dipengaruhi oleh aktiviti antropogenik telah dikaji dengan menggunakan pendekatan penjujukan metagenomik “shotgun” tanpa-kultur. Dua set data metagenomik (~250 GB) dihasilkan melalui platfom “Next-generation Se...

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Main Author:	Foong , Choon Pin
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/31368/1/FOONG_CHOON_PIN_24.pdf
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id	my-usm-ep.31368
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spelling	my-usm-ep.313682019-04-12T05:25:26Z Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes 2016-02 Foong , Choon Pin QH1 Natural history (General - Including nature conservation, geographical distribution) Komuniti mikrob bagi dua tanah paya bakau Pulau Pinang (Batu Maung dan Balik Pulau) yang dipengaruhi oleh aktiviti antropogenik telah dikaji dengan menggunakan pendekatan penjujukan metagenomik “shotgun” tanpa-kultur. Dua set data metagenomik (~250 GB) dihasilkan melalui platfom “Next-generation Sequencing (NGS)” Illumina HiSeq dan disimpan dalam pelayan awam “Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST)”. Analisis taksonomi mikrob menunjukkan bahawa kedua-dua tanah paya bakau Pulau Pinang didominasi oleh Bakteria (97 %), Proteobakteria (43 %) dan Deltaproteobakteria (15 %) pada peringkat domain,. filum dan kelas masing-masing. Pada peringkat genus, kebanyakan bakteria anaerobik diperhatikan terdiri daripada Deltaproteobakteria. Sebahagian besar daripada jujukan adalah milik spesis mikrob (70 %) dan filum (32 %) yang belum dikenalpasti atau belum dikultur. The microbial communities of two local Penang mangrove soils (Batu Maung and Balik Pulau) which are under anthropogenic influences were investigated using culture-independent shotgun metagenome sequencing approach. Two metagenome data sets (~250 GB) were generated from the Illumina HiSeq next-generation sequencing (NGS) platform and then deposited in Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST) public server. Microbial taxonomic analysis showed that both Penang mangrove soils were dominated by Bacteria (97 %), Proteobacteria (43 %) and Deltaproteobacteria (15 %) at the domain, phylum and class levels, respectively. At the genus level, predominance of anaerobic bacteria was observed and mostly belonged to Deltaproteobacteria. A large portion of the reads belonged to unknown or yet uncultured microbial species (70 %) and microbial phyla (32 %). 2016-02 Thesis http://eprints.usm.my/31368/ http://eprints.usm.my/31368/1/FOONG_CHOON_PIN_24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Foong , Choon Pin Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes
description	Komuniti mikrob bagi dua tanah paya bakau Pulau Pinang (Batu Maung dan Balik Pulau) yang dipengaruhi oleh aktiviti antropogenik telah dikaji dengan menggunakan pendekatan penjujukan metagenomik “shotgun” tanpa-kultur. Dua set data metagenomik (~250 GB) dihasilkan melalui platfom “Next-generation Sequencing (NGS)” Illumina HiSeq dan disimpan dalam pelayan awam “Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST)”. Analisis taksonomi mikrob menunjukkan bahawa kedua-dua tanah paya bakau Pulau Pinang didominasi oleh Bakteria (97 %), Proteobakteria (43 %) dan Deltaproteobakteria (15 %) pada peringkat domain,. filum dan kelas masing-masing. Pada peringkat genus, kebanyakan bakteria anaerobik diperhatikan terdiri daripada Deltaproteobakteria. Sebahagian besar daripada jujukan adalah milik spesis mikrob (70 %) dan filum (32 %) yang belum dikenalpasti atau belum dikultur. The microbial communities of two local Penang mangrove soils (Batu Maung and Balik Pulau) which are under anthropogenic influences were investigated using culture-independent shotgun metagenome sequencing approach. Two metagenome data sets (~250 GB) were generated from the Illumina HiSeq next-generation sequencing (NGS) platform and then deposited in Metagenomic-Rapid Annotations using Subsystems Technology (MG-RAST) public server. Microbial taxonomic analysis showed that both Penang mangrove soils were dominated by Bacteria (97 %), Proteobacteria (43 %) and Deltaproteobacteria (15 %) at the domain, phylum and class levels, respectively. At the genus level, predominance of anaerobic bacteria was observed and mostly belonged to Deltaproteobacteria. A large portion of the reads belonged to unknown or yet uncultured microbial species (70 %) and microbial phyla (32 %).
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Foong , Choon Pin
author_facet	Foong , Choon Pin
author_sort	Foong , Choon Pin
title	Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes
title_short	Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes
title_full	Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes
title_fullStr	Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes
title_full_unstemmed	Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes
title_sort	diversity and characterization of polyhydroxyalkanoate synthase (phac) in seawater and mangrove metagenomes
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/31368/1/FOONG_CHOON_PIN_24.pdf
_version_	1747820409757106176

Diversity And Characterization Of Polyhydroxyalkanoate Synthase (Phac) In Seawater And Mangrove Metagenomes

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