Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves

Penghantaran gen menggunakan peptida melibatkan proses pengenalan gen asing ke dalam sel haiwan dan tumbuhan melalui peptida berfungsi yang mengandungi domain penembus sel dan jujukan polikation. Peptida penembus sel berkebolehan untuk memasuki sel-sel hidup, sementara jujukan polikation berkeboleha...

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Main Author:	Lakshmanan, Manoj Kumar
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/31372/1/MANOJ_KUMAR_LAKSHMANAN_24.pdf
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id	my-usm-ep.31372
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spelling	my-usm-ep.313722019-04-12T05:25:26Z Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves 2016-02 Lakshmanan, Manoj Kumar QH1 Natural history (General - Including nature conservation, geographical distribution) Penghantaran gen menggunakan peptida melibatkan proses pengenalan gen asing ke dalam sel haiwan dan tumbuhan melalui peptida berfungsi yang mengandungi domain penembus sel dan jujukan polikation. Peptida penembus sel berkebolehan untuk memasuki sel-sel hidup, sementara jujukan polikation berkebolehan untuk bercantum dan memadatkan plasmid DNA secara elektrostatik. Dalam kajian ini, potensi peptida gabungan yang melingkungi unjuran polikation dan domain berfungsi dengan berkebolehan untuk menembusi sel hidup telah dibentuk dan diuji sebagai pengangkut gen ke dalam sel tumbuhan. Peptida-peptida gabungan yang mempunyai unjuran polikation, nona-arginin (R9) dan ko-polimer histidin-lisina (KH)9 berserta peptida penembus sel Bp100 (KKLFKKILKYL) dan Tat2 (RKKRRQRRRRKKRRQRRR) telah dicampurkan dengan plasmid DNA (pDNA) yang mengekod Renilla luciferase (RLuc) untuk membentuk kompleks pDNA-peptida pada pelbagai nisbah N/P. Nisbah, N/P merujuk kepada nisbah bilangan kumpulan amina daripada peptide per bilangan kumpulan fosfat daripada pDNA. Peptide-based gene delivery involves introduction of foreign genes into animal and plant cells via functional peptides, containing cell penetrating domains and polycationic sequences. Cell penetrating domains are capable of being internalized into living cells, while polycationic sequences can electrostatically interact and condense DNA. In this study, three fusion peptides consisting of polycationic sequences and functional domains with cell penetrating ability were designed and evaluated as potential gene carriers for plant cells. The fusion peptides, consisting of nona-arginine (R9) and histidine-lysine (KH)9 polycationic sequences as well as Bp100 (KKLFKKILKYL) and Tat2 (RKKRRQRRRRKKRRQRRR) cell penetrating domains respectively were mixed with pDNA encoding Renilla luciferase (RLuc) to form pDNA-peptide complexes at various N/P ratios. Here, N/P ratio refers to the ratio of number of amines from peptides per number of phosphates from pDNA. 2016-02 Thesis http://eprints.usm.my/31372/ http://eprints.usm.my/31372/1/MANOJ_KUMAR_LAKSHMANAN_24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Lakshmanan, Manoj Kumar Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves
description	Penghantaran gen menggunakan peptida melibatkan proses pengenalan gen asing ke dalam sel haiwan dan tumbuhan melalui peptida berfungsi yang mengandungi domain penembus sel dan jujukan polikation. Peptida penembus sel berkebolehan untuk memasuki sel-sel hidup, sementara jujukan polikation berkebolehan untuk bercantum dan memadatkan plasmid DNA secara elektrostatik. Dalam kajian ini, potensi peptida gabungan yang melingkungi unjuran polikation dan domain berfungsi dengan berkebolehan untuk menembusi sel hidup telah dibentuk dan diuji sebagai pengangkut gen ke dalam sel tumbuhan. Peptida-peptida gabungan yang mempunyai unjuran polikation, nona-arginin (R9) dan ko-polimer histidin-lisina (KH)9 berserta peptida penembus sel Bp100 (KKLFKKILKYL) dan Tat2 (RKKRRQRRRRKKRRQRRR) telah dicampurkan dengan plasmid DNA (pDNA) yang mengekod Renilla luciferase (RLuc) untuk membentuk kompleks pDNA-peptida pada pelbagai nisbah N/P. Nisbah, N/P merujuk kepada nisbah bilangan kumpulan amina daripada peptide per bilangan kumpulan fosfat daripada pDNA. Peptide-based gene delivery involves introduction of foreign genes into animal and plant cells via functional peptides, containing cell penetrating domains and polycationic sequences. Cell penetrating domains are capable of being internalized into living cells, while polycationic sequences can electrostatically interact and condense DNA. In this study, three fusion peptides consisting of polycationic sequences and functional domains with cell penetrating ability were designed and evaluated as potential gene carriers for plant cells. The fusion peptides, consisting of nona-arginine (R9) and histidine-lysine (KH)9 polycationic sequences as well as Bp100 (KKLFKKILKYL) and Tat2 (RKKRRQRRRRKKRRQRRR) cell penetrating domains respectively were mixed with pDNA encoding Renilla luciferase (RLuc) to form pDNA-peptide complexes at various N/P ratios. Here, N/P ratio refers to the ratio of number of amines from peptides per number of phosphates from pDNA.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lakshmanan, Manoj Kumar
author_facet	Lakshmanan, Manoj Kumar
author_sort	Lakshmanan, Manoj Kumar
title	Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves
title_short	Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves
title_full	Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves
title_fullStr	Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves
title_full_unstemmed	Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves
title_sort	evaluation of fusion peptides as novel gene carriers into nicotiana benthamiana and arabidopsis thaliana leaves
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/31372/1/MANOJ_KUMAR_LAKSHMANAN_24.pdf
_version_	1747820410730184704

Evaluation Of Fusion Peptides As Novel Gene Carriers Into Nicotiana Benthamiana And Arabidopsis Thaliana Leaves

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