Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King

Keperluan untuk mendapatkan pigmen semula jadi daripada sumber mikrob telah berkembang dengan pesatnya memandangkan kebanyakam pigmen sintetik didapati merbahaya kepada manusia. Namun begitu, banyak pigmen semula jadi daripada sumber alamiah yang lain adalah tidak mencukupi untuk memenuhi keperluan...

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Main Author:	Iu , Chai Woei
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/31531/1/IU_CHAI_WOEI_24.pdf
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id	my-usm-ep.31531
record_format	uketd_dc
spelling	my-usm-ep.315312019-04-12T05:25:22Z Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King 2016-03 Iu , Chai Woei QH1 Natural history (General - Including nature conservation, geographical distribution) Keperluan untuk mendapatkan pigmen semula jadi daripada sumber mikrob telah berkembang dengan pesatnya memandangkan kebanyakam pigmen sintetik didapati merbahaya kepada manusia. Namun begitu, banyak pigmen semula jadi daripada sumber alamiah yang lain adalah tidak mencukupi untuk memenuhi keperluan industri. Dalam penyelidikan ini, enam pencilan kulat endofitik yang dipencilkan daripada daun Swietenia macrophylla telah dipilih sebagai subjek kajian tahap awal dan Nigrospora sphaerica CL – OP 30 telah dipilih sebagai sasaran tunggal kajian ini disebabkan oleh keupayaan kulat tersebut menghasilkan pigmen merah yang paling tinggi pada hari ke-14 dengan hasilan sebanyak 0.1471 OD/g berbanding dengan pencilan lain. Kulat tersebut didapati menghasilkan lebih banyak pigmen merah dalam keadaan pengkulturan bercahaya tanpa kehadiran ekstrak tumbuhan perumahnya dan saiz inoculum terbaik adalah dengan kehadiran dua plag kulat berdiameter 1 cm. The demand for natural pigment from microbial sources are growing rapidly since most of the synthetic pigments are found to be hazardous to mankind, and most of the natural pigments obtained from other natural sources are insufficient for the industrial purposes. In this study, six endophytic fungal isolates which were isolated from Swietenia macrophylla were selected as the preliminary study subjects where Nigrospora sphaerica CL-OP 30 was eventually selected as the sole target for the study as the fungus produced highest yield of reddish pigment at day 14 with the 0.1471 OD/g of production compared to others. The higher pigmentation was favourable when the fungus was cultivated in light condition without the incorporation of host plant extract with the ideal inoculum size of the fungus, 2 fungal plugs with 1cm in diameter. 2016-03 Thesis http://eprints.usm.my/31531/ http://eprints.usm.my/31531/1/IU_CHAI_WOEI_24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Iu , Chai Woei Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
description	Keperluan untuk mendapatkan pigmen semula jadi daripada sumber mikrob telah berkembang dengan pesatnya memandangkan kebanyakam pigmen sintetik didapati merbahaya kepada manusia. Namun begitu, banyak pigmen semula jadi daripada sumber alamiah yang lain adalah tidak mencukupi untuk memenuhi keperluan industri. Dalam penyelidikan ini, enam pencilan kulat endofitik yang dipencilkan daripada daun Swietenia macrophylla telah dipilih sebagai subjek kajian tahap awal dan Nigrospora sphaerica CL – OP 30 telah dipilih sebagai sasaran tunggal kajian ini disebabkan oleh keupayaan kulat tersebut menghasilkan pigmen merah yang paling tinggi pada hari ke-14 dengan hasilan sebanyak 0.1471 OD/g berbanding dengan pencilan lain. Kulat tersebut didapati menghasilkan lebih banyak pigmen merah dalam keadaan pengkulturan bercahaya tanpa kehadiran ekstrak tumbuhan perumahnya dan saiz inoculum terbaik adalah dengan kehadiran dua plag kulat berdiameter 1 cm. The demand for natural pigment from microbial sources are growing rapidly since most of the synthetic pigments are found to be hazardous to mankind, and most of the natural pigments obtained from other natural sources are insufficient for the industrial purposes. In this study, six endophytic fungal isolates which were isolated from Swietenia macrophylla were selected as the preliminary study subjects where Nigrospora sphaerica CL-OP 30 was eventually selected as the sole target for the study as the fungus produced highest yield of reddish pigment at day 14 with the 0.1471 OD/g of production compared to others. The higher pigmentation was favourable when the fungus was cultivated in light condition without the incorporation of host plant extract with the ideal inoculum size of the fungus, 2 fungal plugs with 1cm in diameter.
format	Thesis
qualification_level	Master's degree
author	Iu , Chai Woei
author_facet	Iu , Chai Woei
author_sort	Iu , Chai Woei
title	Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_short	Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_full	Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_fullStr	Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_full_unstemmed	Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King
title_sort	natural red pigment with antibacterial activity from nigrospora sphaerica cl-op 30, an endophytic fungus isolated from leaf of swietenia macrophylla king
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/31531/1/IU_CHAI_WOEI_24.pdf
_version_	1747820444982968320

Natural Red Pigment With Antibacterial Activity From Nigrospora Sphaerica CL-OP 30, An Endophytic Fungus Isolated From Leaf Of Swietenia Macrophylla King

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