Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews
Vanilla planifolia Andrews ialah ramuan makanan yang mempunyai nilai ekonomi dan kedua paling mahal di dunia. Dalam kajian yang lepas, bahan tambahan organik telah menujuk potensi mengingkatkan perkembangan dan pertumbuhan pokok tumbuhan tetapi kajian yang teliti terhadap V. planifolia terhad. M...
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://eprints.usm.my/31559/1/GOH_FOONG_JING_24.pdf |
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| Summary: | Vanilla planifolia Andrews ialah ramuan makanan yang mempunyai nilai
ekonomi dan kedua paling mahal di dunia. Dalam kajian yang lepas, bahan
tambahan organik telah menujuk potensi mengingkatkan perkembangan dan
pertumbuhan pokok tumbuhan tetapi kajian yang teliti terhadap V. planifolia terhad.
Maka, dalam kajian ini, kesan bahan tambahan organik berbeza yang dipilih telah
dikaji terhadap pertumbuhan dan perkembangan V. planifolia Andrews. Peningkatan
protokol yang lebih baik untuk pertumbuhan tumbuhan dan perkembangan vanila
telah dijalankan dan akhirnya mengkaji perbezaan morfologi anak pokok yang
ditanam secara in vitro dan ex vitro. Pucuk V. planifolia dikultur dalam kekuatan
medium MS yang berbeza, kepekatan BAP dan sukrosa yang berbeza, dan jenis ejen
gel yang berlainan untuk mengkaji medium pertumbuhan yang terbaik. Pertumbuhan
anak pokok V. planifolia direkodkan selepas dirawat selama lapan minggu. Hasil
Kajian menunjukkan pembekalan ½ kekuatan medium MS dan 20 g/L sukrosa
didapati cukup berkesan untuk menggalakkan pertumbuhan pokok.
Vanilla planifolia Andrews is the economically important and second most
expensive food ingredients in the worldwide. In previous studies, organic additives
have shown their potential on promoting plant growth and development, however the
detail studies of organic additive on plant growth and development of V. planifolia
was limited. Thus, in this research, the effects of different selected organic additives
were studied on the plant growth and development of V. planifolia Andrews.
Improvement on the protocol for plant growth and proliferation of vanilla were
carried out and finally to study the morphological differences between the in vitro
and ex vitro plantlets. V. planifolia plant shoots were initially cultured on different
strengths of MS, different concentrations of BAP, sucrose and different types of
gelling to determine the best growth medium. The V. planifolia plantlets growth
were recorded after eight weeks of treatment. Supplemented of ½ strength MS
medium and supplementation of 20 g/L were efficient enough to promoted plant
growth. |
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