Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews

Vanilla planifolia Andrews ialah ramuan makanan yang mempunyai nilai ekonomi dan kedua paling mahal di dunia. Dalam kajian yang lepas, bahan tambahan organik telah menujuk potensi mengingkatkan perkembangan dan pertumbuhan pokok tumbuhan tetapi kajian yang teliti terhadap V. planifolia terhad. M...

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Main Author:	Goh, Foong Jing
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/31559/1/GOH_FOONG_JING_24.pdf
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spelling	my-usm-ep.315592019-04-12T05:25:22Z Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews 2016-03 Goh, Foong Jing QH1 Natural history (General - Including nature conservation, geographical distribution) Vanilla planifolia Andrews ialah ramuan makanan yang mempunyai nilai ekonomi dan kedua paling mahal di dunia. Dalam kajian yang lepas, bahan tambahan organik telah menujuk potensi mengingkatkan perkembangan dan pertumbuhan pokok tumbuhan tetapi kajian yang teliti terhadap V. planifolia terhad. Maka, dalam kajian ini, kesan bahan tambahan organik berbeza yang dipilih telah dikaji terhadap pertumbuhan dan perkembangan V. planifolia Andrews. Peningkatan protokol yang lebih baik untuk pertumbuhan tumbuhan dan perkembangan vanila telah dijalankan dan akhirnya mengkaji perbezaan morfologi anak pokok yang ditanam secara in vitro dan ex vitro. Pucuk V. planifolia dikultur dalam kekuatan medium MS yang berbeza, kepekatan BAP dan sukrosa yang berbeza, dan jenis ejen gel yang berlainan untuk mengkaji medium pertumbuhan yang terbaik. Pertumbuhan anak pokok V. planifolia direkodkan selepas dirawat selama lapan minggu. Hasil Kajian menunjukkan pembekalan ½ kekuatan medium MS dan 20 g/L sukrosa didapati cukup berkesan untuk menggalakkan pertumbuhan pokok. Vanilla planifolia Andrews is the economically important and second most expensive food ingredients in the worldwide. In previous studies, organic additives have shown their potential on promoting plant growth and development, however the detail studies of organic additive on plant growth and development of V. planifolia was limited. Thus, in this research, the effects of different selected organic additives were studied on the plant growth and development of V. planifolia Andrews. Improvement on the protocol for plant growth and proliferation of vanilla were carried out and finally to study the morphological differences between the in vitro and ex vitro plantlets. V. planifolia plant shoots were initially cultured on different strengths of MS, different concentrations of BAP, sucrose and different types of gelling to determine the best growth medium. The V. planifolia plantlets growth were recorded after eight weeks of treatment. Supplemented of ½ strength MS medium and supplementation of 20 g/L were efficient enough to promoted plant growth. 2016-03 Thesis http://eprints.usm.my/31559/ http://eprints.usm.my/31559/1/GOH_FOONG_JING_24.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Goh, Foong Jing Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews
description	Vanilla planifolia Andrews ialah ramuan makanan yang mempunyai nilai ekonomi dan kedua paling mahal di dunia. Dalam kajian yang lepas, bahan tambahan organik telah menujuk potensi mengingkatkan perkembangan dan pertumbuhan pokok tumbuhan tetapi kajian yang teliti terhadap V. planifolia terhad. Maka, dalam kajian ini, kesan bahan tambahan organik berbeza yang dipilih telah dikaji terhadap pertumbuhan dan perkembangan V. planifolia Andrews. Peningkatan protokol yang lebih baik untuk pertumbuhan tumbuhan dan perkembangan vanila telah dijalankan dan akhirnya mengkaji perbezaan morfologi anak pokok yang ditanam secara in vitro dan ex vitro. Pucuk V. planifolia dikultur dalam kekuatan medium MS yang berbeza, kepekatan BAP dan sukrosa yang berbeza, dan jenis ejen gel yang berlainan untuk mengkaji medium pertumbuhan yang terbaik. Pertumbuhan anak pokok V. planifolia direkodkan selepas dirawat selama lapan minggu. Hasil Kajian menunjukkan pembekalan ½ kekuatan medium MS dan 20 g/L sukrosa didapati cukup berkesan untuk menggalakkan pertumbuhan pokok. Vanilla planifolia Andrews is the economically important and second most expensive food ingredients in the worldwide. In previous studies, organic additives have shown their potential on promoting plant growth and development, however the detail studies of organic additive on plant growth and development of V. planifolia was limited. Thus, in this research, the effects of different selected organic additives were studied on the plant growth and development of V. planifolia Andrews. Improvement on the protocol for plant growth and proliferation of vanilla were carried out and finally to study the morphological differences between the in vitro and ex vitro plantlets. V. planifolia plant shoots were initially cultured on different strengths of MS, different concentrations of BAP, sucrose and different types of gelling to determine the best growth medium. The V. planifolia plantlets growth were recorded after eight weeks of treatment. Supplemented of ½ strength MS medium and supplementation of 20 g/L were efficient enough to promoted plant growth.
format	Thesis
qualification_level	Master's degree
author	Goh, Foong Jing
author_facet	Goh, Foong Jing
author_sort	Goh, Foong Jing
title	Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews
title_short	Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews
title_full	Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews
title_fullStr	Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews
title_full_unstemmed	Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews
title_sort	effects of selected additives for growth efficiency on in vitro grown vanilla planifolia andrews
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/31559/1/GOH_FOONG_JING_24.pdf
_version_	1747820451085680640

Effects Of Selected Additives For Growth Efficiency On In Vitro Grown Vanilla Planifolia Andrews

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