Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli

Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli

Pengekspresan neuraminidase (NA) virus H5N1 di dalam sistem pengekspresan mikrob yang ringkas dengan menggunakan Kluyveromyces lactis dan Escherichia coli sebagai perumah telah dikaji. Untuk penghasilan protein NA dalam K. lactis, dua jenis fragmen DNA NA terdiri daripada 1) jujukan penuh (1347 b...

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Main Author: Taufik, Noor Zafiah
Format: Thesis
Language:English
Published: 2016
Subjects:
QH1 Natural history (General - Including nature conservation
geographical distribution)
Online Access:http://eprints.usm.my/31644/1/Noor_Zafiah_Taufik.pdf
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spelling my-usm-ep.316442019-04-12T05:25:14Z Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli 2016-09 Taufik, Noor Zafiah QH1 Natural history (General - Including nature conservation, geographical distribution) Pengekspresan neuraminidase (NA) virus H5N1 di dalam sistem pengekspresan mikrob yang ringkas dengan menggunakan Kluyveromyces lactis dan Escherichia coli sebagai perumah telah dikaji. Untuk penghasilan protein NA dalam K. lactis, dua jenis fragmen DNA NA terdiri daripada 1) jujukan penuh (1347 bp) dan 2) jujukan domain kepala (residu 63-449, 1161 bp) NA daripada A/Chicken/Malaysia/5858/2004 (H5N1) telah diamplikasi dan diklon ke dalam vektor pengekspresan pKLAC2 lalu menghasilkan konstruk plasmid yang dinamakan pKLAC2-NA and pKLAC2-NAHD. The expression of H5N1 virus neuraminidase (NA) in simple microbial expression system utilizing Kluyveromyces lactis and Escherichia coli as the host was studied. For NA protein production in K. lactis, two types of NA DNA fragment composed of 1) full length sequence (1347 bp) and 2) head domain sequence (residue 63-449, 1161 bp) from A/Chicken/Malaysia/5858/2004 (H5N1) were amplified and cloned into pKLAC2 expression vector generating plasmid constructs named pKLAC2-NA and pKLAC2-NAHD. 2016-09 Thesis http://eprints.usm.my/31644/ http://eprints.usm.my/31644/1/Noor_Zafiah_Taufik.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1 Natural history (General - Including nature conservation
geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation
geographical distribution)
Taufik, Noor Zafiah
Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli
description Pengekspresan neuraminidase (NA) virus H5N1 di dalam sistem pengekspresan mikrob yang ringkas dengan menggunakan Kluyveromyces lactis dan Escherichia coli sebagai perumah telah dikaji. Untuk penghasilan protein NA dalam K. lactis, dua jenis fragmen DNA NA terdiri daripada 1) jujukan penuh (1347 bp) dan 2) jujukan domain kepala (residu 63-449, 1161 bp) NA daripada A/Chicken/Malaysia/5858/2004 (H5N1) telah diamplikasi dan diklon ke dalam vektor pengekspresan pKLAC2 lalu menghasilkan konstruk plasmid yang dinamakan pKLAC2-NA and pKLAC2-NAHD. The expression of H5N1 virus neuraminidase (NA) in simple microbial expression system utilizing Kluyveromyces lactis and Escherichia coli as the host was studied. For NA protein production in K. lactis, two types of NA DNA fragment composed of 1) full length sequence (1347 bp) and 2) head domain sequence (residue 63-449, 1161 bp) from A/Chicken/Malaysia/5858/2004 (H5N1) were amplified and cloned into pKLAC2 expression vector generating plasmid constructs named pKLAC2-NA and pKLAC2-NAHD.
format Thesis
qualification_level Master's degree
author Taufik, Noor Zafiah
author_facet Taufik, Noor Zafiah
author_sort Taufik, Noor Zafiah
title Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli
title_short Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli
title_full Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli
title_fullStr Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli
title_full_unstemmed Heterologous Expression Of Avian Influenza A (H5n1) Neuraminidase In Kluyveromyces Lactis And Escherichia Coli
title_sort heterologous expression of avian influenza a (h5n1) neuraminidase in kluyveromyces lactis and escherichia coli
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2016
url http://eprints.usm.my/31644/1/Noor_Zafiah_Taufik.pdf
_version_ 1747820470420373504

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