LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy

Suatu kaedah LC/MS-MS untuk mengukur paras homosistein plasma dari sampel manusia telah dihasilkan. Kaedah ini melibatkan penyediaan sampel yang mudah dan telah menunjukkan kepersisan, kespesifikan, kesensitifan dan kejituan yang baik. Dengan itu, kaedah ini boleh digunakan untuk memeriksa aras homo...

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Main Author:	Lim, Luen Hui
Format:	Thesis
Language:	English
Published:	2015
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/31692/1/LIM_LUEN_HUI_24%28NN%29.pdf
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id	my-usm-ep.31692
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spelling	my-usm-ep.316922019-04-12T05:25:35Z LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy 2015-10 Lim, Luen Hui RS1-441 Pharmacy and materia medica Suatu kaedah LC/MS-MS untuk mengukur paras homosistein plasma dari sampel manusia telah dihasilkan. Kaedah ini melibatkan penyediaan sampel yang mudah dan telah menunjukkan kepersisan, kespesifikan, kesensitifan dan kejituan yang baik. Dengan itu, kaedah ini boleh digunakan untuk memeriksa aras homosistein sampel klinikal dalam bilangan yang besar. Di samping itu, kaedah ini juga dibandingkan dengan kaedah imunoasai yang digunakan oleh makmal patologi komersial untuk pengukuran paras homosistein. Kedua-dua kaedah ini menunjukkan kejituan yang baik dan min perbezaan yang rendah antara satu sama lain. Dua puluh enam peratus daripada 260 pesakit kencing manis dengan neuropati peripheral yang direkrut mempunyai paras homosistein yang tinggi. Terdapat lebih ramai lelaki daripada perempuan di dalam kumpulan hiperhomosistein (homosistein ≥15μM). Paras homosistein adalah berkaitan secara langsung dengan paras kreatinina pesakit kajian ini, manakala paras vitamin B12 dan asid folik adalah berhubungan songsang dengan paras homosistein pesakit. A simple LC-MS/MS method was developed to measure homocysteine level in human plasma collected from diabetic patients. This method which involves simple sample preparation showed good precision, specificity, sensitivity and accuracy. Thus, it can be used to screen a vast number of clinical samples. This method‘s results were compared with commercial laboratory results derived from the immunoassay method. Both methods showed good accuracy with low mean difference. Of the 260 patients recruited with peripheral neuropathy, 26% showed hyperhomocysteinemia (homocysteine ≥15μM). The male gender was more prominent in the hyperhomocysteine group. Homocysteine level was directly associated with creatinine, while homocysteine level was inversely correlated with the vitamin status (B12 and folic acid). 2015-10 Thesis http://eprints.usm.my/31692/ http://eprints.usm.my/31692/1/LIM_LUEN_HUI_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Lim, Luen Hui LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy
description	Suatu kaedah LC/MS-MS untuk mengukur paras homosistein plasma dari sampel manusia telah dihasilkan. Kaedah ini melibatkan penyediaan sampel yang mudah dan telah menunjukkan kepersisan, kespesifikan, kesensitifan dan kejituan yang baik. Dengan itu, kaedah ini boleh digunakan untuk memeriksa aras homosistein sampel klinikal dalam bilangan yang besar. Di samping itu, kaedah ini juga dibandingkan dengan kaedah imunoasai yang digunakan oleh makmal patologi komersial untuk pengukuran paras homosistein. Kedua-dua kaedah ini menunjukkan kejituan yang baik dan min perbezaan yang rendah antara satu sama lain. Dua puluh enam peratus daripada 260 pesakit kencing manis dengan neuropati peripheral yang direkrut mempunyai paras homosistein yang tinggi. Terdapat lebih ramai lelaki daripada perempuan di dalam kumpulan hiperhomosistein (homosistein ≥15μM). Paras homosistein adalah berkaitan secara langsung dengan paras kreatinina pesakit kajian ini, manakala paras vitamin B12 dan asid folik adalah berhubungan songsang dengan paras homosistein pesakit. A simple LC-MS/MS method was developed to measure homocysteine level in human plasma collected from diabetic patients. This method which involves simple sample preparation showed good precision, specificity, sensitivity and accuracy. Thus, it can be used to screen a vast number of clinical samples. This method‘s results were compared with commercial laboratory results derived from the immunoassay method. Both methods showed good accuracy with low mean difference. Of the 260 patients recruited with peripheral neuropathy, 26% showed hyperhomocysteinemia (homocysteine ≥15μM). The male gender was more prominent in the hyperhomocysteine group. Homocysteine level was directly associated with creatinine, while homocysteine level was inversely correlated with the vitamin status (B12 and folic acid).
format	Thesis
qualification_level	Master's degree
author	Lim, Luen Hui
author_facet	Lim, Luen Hui
author_sort	Lim, Luen Hui
title	LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy
title_short	LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy
title_full	LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy
title_fullStr	LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy
title_full_unstemmed	LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy
title_sort	lc-ms/ms analysis of plasma homocysteine and investigation on the association of homocysteine levels with diabetic peripheral neuropathy
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
publishDate	2015
url	http://eprints.usm.my/31692/1/LIM_LUEN_HUI_24%28NN%29.pdf
_version_	1747820478251139072

LC-MS/MS Analysis Of Plasma Homocysteine And Investigation On The Association Of Homocysteine Levels With Diabetic Peripheral Neuropathy

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