Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis

Getah asli daripada Hevea brasiliensis yang diperbuat daripada cis-1,4-poliisopren, merupakan komoditi perusahaan yang penuh dengan ciri-ciri keunikan. Biosintesis getah asli daripada H. brasiliensis berlaku pada permukaan partikel getah yang dimangkinkan oleh ‘small rubber particle protein’ (SRPP)...

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Main Author:	Satinder Singh, Saranpal Singh
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/31810/1/SARANPAL_SINGH_AL_SATINDER_SINGH_24%28NN%29.pdf
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id	my-usm-ep.31810
record_format	uketd_dc
spelling	my-usm-ep.318102019-04-12T05:25:26Z Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis 2016-02 Satinder Singh, Saranpal Singh QH1 Natural history (General - Including nature conservation, geographical distribution) Getah asli daripada Hevea brasiliensis yang diperbuat daripada cis-1,4-poliisopren, merupakan komoditi perusahaan yang penuh dengan ciri-ciri keunikan. Biosintesis getah asli daripada H. brasiliensis berlaku pada permukaan partikel getah yang dimangkinkan oleh ‘small rubber particle protein’ (SRPP) dan ‘rubber elongation factor’ (REF). Protein SRPP ini dipercayai menggabungkan isopentenil difosfat (IPP) pada permukaan partikel getah. Tambahan pula, protein SRPP juga merupakan salah satu alergen getah utama dalam H. brasiliensis. Walaupun terdapat banyak bukti yang menunjukkan penglibatan protein ini dalam biosintesis getah dan alergen, namun, masih terdapat kekurangan penyelidikan yang mengaitkan mekanisme SRPP dalam sintesis getah dan mekanisme alahan. Natural rubber (NR) of Hevea brasiliensis predominantly made up of cis-1,4-polyisoprene, is an essential industrial commodity with unique characteristics. The biosynthesis of NR from H. brasiliensis is catalyzed on the surface of rubber particles by a set of integrated proteins, namely the small rubber particle protein (SRPP), and rubber elongation factor (REF). The SRPP protein has been speculated to incorporate isopentenyl diphosphate (IPP) monomers in rubber particles. Furthermore, the SRPP protein has also been implicated as a major latex allergen. 2016-02 Thesis http://eprints.usm.my/31810/ http://eprints.usm.my/31810/1/SARANPAL_SINGH_AL_SATINDER_SINGH_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Satinder Singh, Saranpal Singh Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis
description	Getah asli daripada Hevea brasiliensis yang diperbuat daripada cis-1,4-poliisopren, merupakan komoditi perusahaan yang penuh dengan ciri-ciri keunikan. Biosintesis getah asli daripada H. brasiliensis berlaku pada permukaan partikel getah yang dimangkinkan oleh ‘small rubber particle protein’ (SRPP) dan ‘rubber elongation factor’ (REF). Protein SRPP ini dipercayai menggabungkan isopentenil difosfat (IPP) pada permukaan partikel getah. Tambahan pula, protein SRPP juga merupakan salah satu alergen getah utama dalam H. brasiliensis. Walaupun terdapat banyak bukti yang menunjukkan penglibatan protein ini dalam biosintesis getah dan alergen, namun, masih terdapat kekurangan penyelidikan yang mengaitkan mekanisme SRPP dalam sintesis getah dan mekanisme alahan. Natural rubber (NR) of Hevea brasiliensis predominantly made up of cis-1,4-polyisoprene, is an essential industrial commodity with unique characteristics. The biosynthesis of NR from H. brasiliensis is catalyzed on the surface of rubber particles by a set of integrated proteins, namely the small rubber particle protein (SRPP), and rubber elongation factor (REF). The SRPP protein has been speculated to incorporate isopentenyl diphosphate (IPP) monomers in rubber particles. Furthermore, the SRPP protein has also been implicated as a major latex allergen.
format	Thesis
qualification_level	Master's degree
author	Satinder Singh, Saranpal Singh
author_facet	Satinder Singh, Saranpal Singh
author_sort	Satinder Singh, Saranpal Singh
title	Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis
title_short	Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis
title_full	Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis
title_fullStr	Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis
title_full_unstemmed	Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis
title_sort	expression, purification and crystallization trials of small rubber particle protein (srpp) from hevea brasiliensis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/31810/1/SARANPAL_SINGH_AL_SATINDER_SINGH_24%28NN%29.pdf
_version_	1747820492409012224

Expression, Purification And Crystallization Trials Of Small Rubber Particle Protein (Srpp) From Hevea Brasiliensis

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