Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies

Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format ut...

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Main Author:	Ismail, Nur Faezee
Format:	Thesis
Language:	English
Published:	2016
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf
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id	my-usm-ep.31823
record_format	uketd_dc
spelling	my-usm-ep.318232019-04-12T05:25:22Z Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies 2016-03 Ismail, Nur Faezee R735-854 Medical education. Medical schools. Research Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format utama POC asai adalah pengesanan interaksi antibodi- antigen . Untuk mencapai matlamat ini, asai yang mampu melaporkan interaksi antara antibodi dan antigen adalah diperlukan. Kebiasaannya. asai dibangunkan dengan merangkaikan antibodi-antibodi kepada satu pelapor yang spesifik seperti enzim atau protein. Projek ini menerangkan penggunaan transpeptidasi berantarakan sortase sebagai satu cara untuk merangkaikan antibodi rekombinan untuk sepasang protein pelapor. Enzim Sortase adalah unik dengan mengenali motif yang mempunyai urutan yang khusus untuk membenarkan tidak balas rangkaian protein-protein secara setempat. Point-of-care (POC) diagnostics for resource deprived regions remains a major challenge to combat infectious diseases worldwide. Development of new methods to aid the development process of new POC diagnostics is essential to improve the healthcare scenario worldwide. The major format of POC assay is the detection of antibody-antigen interaction. To achieve this, an assay is required to be capable of reporting the interaction between the antibody and antigen. In most common assays, these detection are achieved by conjugating antibodies to specific reporter molecules such as enzymes or proteins. The work describes the application of sortase mediated transpeptidation as a means to conjugate recombinant antibodies to a pair of reporter proteins. 2016-03 Thesis http://eprints.usm.my/31823/ http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul (INFORMM)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Ismail, Nur Faezee Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
description	Diagnostik ‘point-of-care’ (POC) untuk kawasan terpencil telah menjadi cabaran besar untuk memerangi penyakit berjangkit di seluruh dunia. Pembangunan kaedah untuk membantu proses pembangunan diagnostik POC baru adalah penting untuk memperbaiki senario penjagaan kesihatan di seluruh dunia. Format utama POC asai adalah pengesanan interaksi antibodi- antigen . Untuk mencapai matlamat ini, asai yang mampu melaporkan interaksi antara antibodi dan antigen adalah diperlukan. Kebiasaannya. asai dibangunkan dengan merangkaikan antibodi-antibodi kepada satu pelapor yang spesifik seperti enzim atau protein. Projek ini menerangkan penggunaan transpeptidasi berantarakan sortase sebagai satu cara untuk merangkaikan antibodi rekombinan untuk sepasang protein pelapor. Enzim Sortase adalah unik dengan mengenali motif yang mempunyai urutan yang khusus untuk membenarkan tidak balas rangkaian protein-protein secara setempat. Point-of-care (POC) diagnostics for resource deprived regions remains a major challenge to combat infectious diseases worldwide. Development of new methods to aid the development process of new POC diagnostics is essential to improve the healthcare scenario worldwide. The major format of POC assay is the detection of antibody-antigen interaction. To achieve this, an assay is required to be capable of reporting the interaction between the antibody and antigen. In most common assays, these detection are achieved by conjugating antibodies to specific reporter molecules such as enzymes or proteins. The work describes the application of sortase mediated transpeptidation as a means to conjugate recombinant antibodies to a pair of reporter proteins.
format	Thesis
qualification_level	Master's degree
author	Ismail, Nur Faezee
author_facet	Ismail, Nur Faezee
author_sort	Ismail, Nur Faezee
title	Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_short	Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_full	Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_fullStr	Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_full_unstemmed	Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies
title_sort	sortase– mediated transpeptidation: the application of sortase a as a tool for site- specific modification of recombinant antibodies
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul (INFORMM)
publishDate	2016
url	http://eprints.usm.my/31823/1/NUR_FAEZEE_BINTI_ISMAIL_24%28NN%29.pdf
_version_	1747820494616264704

Sortase– Mediated Transpeptidation: The Application Of Sortase A As A Tool For Site- Specific Modification Of Recombinant Antibodies

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