Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development

Strongyloidiasis merupakan penyakit parasit manusia yang disebabkan oleh cacing Strongyloides stercoralis. Jangkitan daripada cacing ini akan menyebabkan jangkitan jangka panjang dalam manusia, atau boleh disebarkan ke organ-organ lain, terutama bagi individu dengan sistem imun terkompromi, yang...

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Main Author:	Arifin, Norsyahida
Format:	Thesis
Language:	English
Published:	2016
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/31824/1/NORSYAHIDA_BINTI_ARIFIN_24%28NN%29.pdf
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spelling	my-usm-ep.318242019-04-12T05:25:22Z Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development 2016-03 Arifin, Norsyahida R735-854 Medical education. Medical schools. Research Strongyloidiasis merupakan penyakit parasit manusia yang disebabkan oleh cacing Strongyloides stercoralis. Jangkitan daripada cacing ini akan menyebabkan jangkitan jangka panjang dalam manusia, atau boleh disebarkan ke organ-organ lain, terutama bagi individu dengan sistem imun terkompromi, yang biasanya mengakibatkan kematian. Majoriti pesakit adalah tidak bersimptom, atau mengalami masalah gastrousus tak-spesifik, namun masih tiada kaedah ‘gold standard’ untuk mengesan jangkitan tersebut. Diagnosis definitif biasanya dilakukan melalui kombinasi tanda dan simptom klinikal, pengesanan mikroskopik, dan ujian serologi. Sehingga kini, ujian komersil yang sedia ada adalah berasaskan ekstrak asli antigen parasit, tetapi ujian tersebut mempunyai banyak kelemahan; contohnya masalah reaktiviti silang dengan jangkitan cacing yang lain. Ujian berasaskan antigen rekombinan merupakan alternatif yang sesuai bagi meningkatkan spesifisiti diagnostik dan keseragaman kualiti ujian, oleh itu, kajian ini dijalankan untuk mencapai matlamat ini. Peringkat awal kajian ini melibatkan pengujian sampel serum dengan in-house IgG-, IgG4- dan IgE-ELISA disamping kit komersial IgG-ELISA. Strongyloidiasis is a human parasitic disease caused by the nematode Strongyloides stercoralis. Infection by this parasite can cause long-term infection in humans or can disseminate to other organs, especially in individuals with immunosuppression, which commonly results in fatal outcomes. The majority of patients are asymptomatic or present with non-specific gastrointestinal complaints, and there is no gold standard method to rule out the infection. Definitive diagnosis is usually made by a combination of clinical signs and symptoms, microscopic identification, and serology test. To date, the available commercial tests are based on native parasite antigen extract, but such tests have problems of cross-reactivity with other helminthic infections. A recombinant antigen-based test is a good alternative for improved diagnostic specificity and standardized test quality, thus, the present study was conducted to achieve this goal. The initial stage of the study involved testing serum samples with the in-house IgG-, IgG4- and IgE-ELISAs in addition to a commercial IgG-ELISA kit. 2016-03 Thesis http://eprints.usm.my/31824/ http://eprints.usm.my/31824/1/NORSYAHIDA_BINTI_ARIFIN_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul (INFORMM)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Arifin, Norsyahida Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development
description	Strongyloidiasis merupakan penyakit parasit manusia yang disebabkan oleh cacing Strongyloides stercoralis. Jangkitan daripada cacing ini akan menyebabkan jangkitan jangka panjang dalam manusia, atau boleh disebarkan ke organ-organ lain, terutama bagi individu dengan sistem imun terkompromi, yang biasanya mengakibatkan kematian. Majoriti pesakit adalah tidak bersimptom, atau mengalami masalah gastrousus tak-spesifik, namun masih tiada kaedah ‘gold standard’ untuk mengesan jangkitan tersebut. Diagnosis definitif biasanya dilakukan melalui kombinasi tanda dan simptom klinikal, pengesanan mikroskopik, dan ujian serologi. Sehingga kini, ujian komersil yang sedia ada adalah berasaskan ekstrak asli antigen parasit, tetapi ujian tersebut mempunyai banyak kelemahan; contohnya masalah reaktiviti silang dengan jangkitan cacing yang lain. Ujian berasaskan antigen rekombinan merupakan alternatif yang sesuai bagi meningkatkan spesifisiti diagnostik dan keseragaman kualiti ujian, oleh itu, kajian ini dijalankan untuk mencapai matlamat ini. Peringkat awal kajian ini melibatkan pengujian sampel serum dengan in-house IgG-, IgG4- dan IgE-ELISA disamping kit komersial IgG-ELISA. Strongyloidiasis is a human parasitic disease caused by the nematode Strongyloides stercoralis. Infection by this parasite can cause long-term infection in humans or can disseminate to other organs, especially in individuals with immunosuppression, which commonly results in fatal outcomes. The majority of patients are asymptomatic or present with non-specific gastrointestinal complaints, and there is no gold standard method to rule out the infection. Definitive diagnosis is usually made by a combination of clinical signs and symptoms, microscopic identification, and serology test. To date, the available commercial tests are based on native parasite antigen extract, but such tests have problems of cross-reactivity with other helminthic infections. A recombinant antigen-based test is a good alternative for improved diagnostic specificity and standardized test quality, thus, the present study was conducted to achieve this goal. The initial stage of the study involved testing serum samples with the in-house IgG-, IgG4- and IgE-ELISAs in addition to a commercial IgG-ELISA kit.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Arifin, Norsyahida
author_facet	Arifin, Norsyahida
author_sort	Arifin, Norsyahida
title	Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development
title_short	Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development
title_full	Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development
title_fullStr	Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development
title_full_unstemmed	Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development
title_sort	serodiagnosis of strongyloidiasis: identification of cdna clones, production of recombinant antigens and immunoassay development
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul (INFORMM)
publishDate	2016
url	http://eprints.usm.my/31824/1/NORSYAHIDA_BINTI_ARIFIN_24%28NN%29.pdf
_version_	1747820494858485760

Serodiagnosis Of Strongyloidiasis: Identification Of Cdna Clones, Production Of Recombinant Antigens And Immunoassay Development

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