Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media

SecB adalah salah satu komponen utama dalam sistem translokasi protein Sec terutamanya bagi bakteria Gram-negatif. Dalam kajian ini, fungsi SecB telah dikaji dengan membina mutan Cupriavidus necator H16 ΔsecB. Mutan ini telah dibina melalui pertukaran alel menggunakan vektor swa-hapus pDM4. Untuk...

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Main Author:	Sim , Xuan Yi
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/31855/1/SIM_XUAN_YI_24%28NN%29.pdf
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id	my-usm-ep.31855
record_format	uketd_dc
spelling	my-usm-ep.318552019-04-12T05:25:25Z Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media 2016-03 Sim , Xuan Yi QH1 Natural history (General - Including nature conservation, geographical distribution) SecB adalah salah satu komponen utama dalam sistem translokasi protein Sec terutamanya bagi bakteria Gram-negatif. Dalam kajian ini, fungsi SecB telah dikaji dengan membina mutan Cupriavidus necator H16 ΔsecB. Mutan ini telah dibina melalui pertukaran alel menggunakan vektor swa-hapus pDM4. Untuk mengesahkan perubahan fenotip disebabkan oleh gen yang dimutasi, mutan pelengkap juga dibina, yang mana vektor pBBR1MCS2 yang mengandungi gen SecB telah dikonjugasi ke dalam mutan C. necator H16 ΔsecB. Jenis liar, C. necator H16 ΔsecB mutan dan mutan pelengkap telah dikaji mengguna sistem Biolog Phenotype Microarray. Mutan pelengkap menunjukkan fenotip jenis liar, bermakna penglengkapan secB telah berjaya. Mutan Cupriavidus necator H16 ΔsecB tidak menunjukkan perbezaan yang signifikan apabila ditumbuh dialam kebanyakan substrat. SecB is one of the key players in the Sec protein-translocation system ubiquitous to Gram-negative bacteria. In this study the function of SecB was studied by studying an unmarked Cupriavidus necator H16 ΔsecB mutant. The mutant was constructed through allelic exchange utilizing the pDM4 suicidal vector. In order to confirm that the phenotypic change is due to the mutated gene, a complementation mutant was also constructed, in which vector pBBR1MCS2 harbouring a secB gene was introduced into the C. necator H16 ΔsecB mutant via conjugation. The wild type, C. necator H16 ΔsecB mutant and the complementation mutant were characterised using Biolog Phenotype Microarray. The result shows that the complementation mutant exhibits the wild type phenotype, indicating successful complementation of the unmarked secB deletion. The C. necator H16 ΔsecB mutant did not show any significant difference when grown on most substrate. 2016-03 Thesis http://eprints.usm.my/31855/ http://eprints.usm.my/31855/1/SIM_XUAN_YI_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Sim , Xuan Yi Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media
description	SecB adalah salah satu komponen utama dalam sistem translokasi protein Sec terutamanya bagi bakteria Gram-negatif. Dalam kajian ini, fungsi SecB telah dikaji dengan membina mutan Cupriavidus necator H16 ΔsecB. Mutan ini telah dibina melalui pertukaran alel menggunakan vektor swa-hapus pDM4. Untuk mengesahkan perubahan fenotip disebabkan oleh gen yang dimutasi, mutan pelengkap juga dibina, yang mana vektor pBBR1MCS2 yang mengandungi gen SecB telah dikonjugasi ke dalam mutan C. necator H16 ΔsecB. Jenis liar, C. necator H16 ΔsecB mutan dan mutan pelengkap telah dikaji mengguna sistem Biolog Phenotype Microarray. Mutan pelengkap menunjukkan fenotip jenis liar, bermakna penglengkapan secB telah berjaya. Mutan Cupriavidus necator H16 ΔsecB tidak menunjukkan perbezaan yang signifikan apabila ditumbuh dialam kebanyakan substrat. SecB is one of the key players in the Sec protein-translocation system ubiquitous to Gram-negative bacteria. In this study the function of SecB was studied by studying an unmarked Cupriavidus necator H16 ΔsecB mutant. The mutant was constructed through allelic exchange utilizing the pDM4 suicidal vector. In order to confirm that the phenotypic change is due to the mutated gene, a complementation mutant was also constructed, in which vector pBBR1MCS2 harbouring a secB gene was introduced into the C. necator H16 ΔsecB mutant via conjugation. The wild type, C. necator H16 ΔsecB mutant and the complementation mutant were characterised using Biolog Phenotype Microarray. The result shows that the complementation mutant exhibits the wild type phenotype, indicating successful complementation of the unmarked secB deletion. The C. necator H16 ΔsecB mutant did not show any significant difference when grown on most substrate.
format	Thesis
qualification_level	Master's degree
author	Sim , Xuan Yi
author_facet	Sim , Xuan Yi
author_sort	Sim , Xuan Yi
title	Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media
title_short	Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media
title_full	Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media
title_fullStr	Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media
title_full_unstemmed	Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media
title_sort	sodium cloride and sucrose rescued cupriavidus necator h16 δsecb mutant when grown in nutrient rich media
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/31855/1/SIM_XUAN_YI_24%28NN%29.pdf
_version_	1747820498277892096

Sodium Cloride And Sucrose Rescued Cupriavidus Necator H16 Δsecb Mutant When Grown In Nutrient Rich Media

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