Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro

Latar Belakang: Sel-sel tunjang mesenkima (MSCs) terbukti dalam membantu proses pembaikan jantung melalui pengaktifan sel-sel jantung (CSCs) berikutan infaksi, tetapi pengeksploitasian secara in vitro melibatkan sinergi antara dua sel sebelum pemindahan sel masih kurang diketahui. Objektif: Kajian i...

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Main Author:	Leong, Yin Yee
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RK1-715 Dentistry
Online Access:	http://eprints.usm.my/32076/1/LEONG_YIN_YEE_24%28NN%29.pdf
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id	my-usm-ep.32076
record_format	uketd_dc
spelling	my-usm-ep.320762019-04-12T05:25:27Z Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro 2016-01 Leong, Yin Yee RK1-715 Dentistry Latar Belakang: Sel-sel tunjang mesenkima (MSCs) terbukti dalam membantu proses pembaikan jantung melalui pengaktifan sel-sel jantung (CSCs) berikutan infaksi, tetapi pengeksploitasian secara in vitro melibatkan sinergi antara dua sel sebelum pemindahan sel masih kurang diketahui. Objektif: Kajian ini bertujuan untuk mengkaji kesan sinergi MSCs kepada diferensiasi sel kardiomiosit. Kaedah: CSCs yang mengekspres c-kit dan MSCs tulang diperoleh daripada mencit C57BL/6N (n=9). Diferensiasi CSCs diuji selepas diasingkan daripada MSCs berikutan ko-kultur dengan menggunakan zarah oksida besi bersaiz micron, dan dibandingkan dengan CSCs yang dirawat dengan medium terkondisi MSCs atau tanpa sebarang rawatan. Diferensiasi kardiomiosit dikesan menggunakan penwarnaan imunofluorescein dan diukur menggunakan tindakbalas polimerase berantai secara quantitatif. Semua data dianalis dengan ANOVA sehala. Background: Bone marrow-derived mesenchymal stem cells (MSCs) has been shown to facilitate heart repair via activation of endogenous cardiac stem cells (CSCs) following infarction, but little is known if the synergy of the two cells can be exploited in vitro prior to transplantation. Objective: This study aimed to examine the synergistic effects of MSCs on CSC cardiomyocyte differentiation. Methods: C-kit CSCs and MSCs were isolated from 4-6 weeks C57BL/6N mice (n=9). CSC cardiomyocyte differentiation was tested after re-isolated from MSC co-culture using micron-sized particles of iron oxide, and compared to CSCs treated with MSC-conditioned medium (CdM) or without any treatment. Cardiomyocyte differentiation was detected using immunofluorescence staining and quantified using qPCR. All data were analysed by one way ANOVA. 2016-01 Thesis http://eprints.usm.my/32076/ http://eprints.usm.my/32076/1/LEONG_YIN_YEE_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK1-715 Dentistry
spellingShingle	RK1-715 Dentistry Leong, Yin Yee Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro
description	Latar Belakang: Sel-sel tunjang mesenkima (MSCs) terbukti dalam membantu proses pembaikan jantung melalui pengaktifan sel-sel jantung (CSCs) berikutan infaksi, tetapi pengeksploitasian secara in vitro melibatkan sinergi antara dua sel sebelum pemindahan sel masih kurang diketahui. Objektif: Kajian ini bertujuan untuk mengkaji kesan sinergi MSCs kepada diferensiasi sel kardiomiosit. Kaedah: CSCs yang mengekspres c-kit dan MSCs tulang diperoleh daripada mencit C57BL/6N (n=9). Diferensiasi CSCs diuji selepas diasingkan daripada MSCs berikutan ko-kultur dengan menggunakan zarah oksida besi bersaiz micron, dan dibandingkan dengan CSCs yang dirawat dengan medium terkondisi MSCs atau tanpa sebarang rawatan. Diferensiasi kardiomiosit dikesan menggunakan penwarnaan imunofluorescein dan diukur menggunakan tindakbalas polimerase berantai secara quantitatif. Semua data dianalis dengan ANOVA sehala. Background: Bone marrow-derived mesenchymal stem cells (MSCs) has been shown to facilitate heart repair via activation of endogenous cardiac stem cells (CSCs) following infarction, but little is known if the synergy of the two cells can be exploited in vitro prior to transplantation. Objective: This study aimed to examine the synergistic effects of MSCs on CSC cardiomyocyte differentiation. Methods: C-kit CSCs and MSCs were isolated from 4-6 weeks C57BL/6N mice (n=9). CSC cardiomyocyte differentiation was tested after re-isolated from MSC co-culture using micron-sized particles of iron oxide, and compared to CSCs treated with MSC-conditioned medium (CdM) or without any treatment. Cardiomyocyte differentiation was detected using immunofluorescence staining and quantified using qPCR. All data were analysed by one way ANOVA.
format	Thesis
qualification_level	Master's degree
author	Leong, Yin Yee
author_facet	Leong, Yin Yee
author_sort	Leong, Yin Yee
title	Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro
title_short	Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro
title_full	Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro
title_fullStr	Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro
title_full_unstemmed	Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro
title_sort	interaction between murine cardiac stem cells and bone marrow-derived mesenchymal stem cells for cardiomyocyte differentiation in vitro
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
publishDate	2016
url	http://eprints.usm.my/32076/1/LEONG_YIN_YEE_24%28NN%29.pdf
_version_	1747820522317545472

Interaction Between Murine Cardiac Stem Cells And Bone Marrow-Derived Mesenchymal Stem Cells For Cardiomyocyte Differentiation In Vitro

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