Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity

Lippia nodiflora telah digunakan secara tradisional dalam Ayurveda, Unani, Sindh, dan Perubatan Tradisional Cina untuk rawatan sakit lutut sendi, lithiasis, diuresis, penyakit urinari dan bengkak. Dalam kajian ini, ekstrak metanol L. nodiflora menunjukkan aktiviti perencatan xantina oksidase (XOD)...

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Main Author:	Cheng, Lee Chuen
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/32134/1/CHENG_LEE_CHUEN_24%28NN%29.pdf
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spelling	my-usm-ep.321342019-04-12T05:25:17Z Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity 2016-07 Cheng, Lee Chuen RS1-441 Pharmacy and materia medica Lippia nodiflora telah digunakan secara tradisional dalam Ayurveda, Unani, Sindh, dan Perubatan Tradisional Cina untuk rawatan sakit lutut sendi, lithiasis, diuresis, penyakit urinari dan bengkak. Dalam kajian ini, ekstrak metanol L. nodiflora menunjukkan aktiviti perencatan xantina oksidase (XOD) secara in vitro baik. Pemeringkatan berpandukan bioaktiviti ekstrak metanol menghasilkan empat pecahan (F1-F4) dengan F3 dikenal pasti sebagai pecahan yang paling poten. Penulenan F3 selanjutnya menghasilkan lima sebatian bioaktif, termasuk dua feniletanoid glikosida arenariosida (1) dan verbaskosida (2), dan tiga flavonoid 6-hidroksiluteolin (3), 6-hidroksiluteolin-7-O-glikosida (4), dan nodifloretin (5), di mana 1 dan 4 diasingkan untuk kali pertama daripada L. nodiflora. Ekstrak metanol, pecahan-pecahan, dan juzuk kimia tersebut kemudiannya diuji untuk activiti antihiperurisemik berpotensi secara in vivo dengan menggunakan model tikus hiperurisemik diaruh oleh kalium oxonate dan hipoksantina. Rawatan oral ekstrak metanol menurunkan paras asid urik serum tikus hiperurisemik dengan berkesan dan bersandarkan dos. Lippia nodiflora has been traditionally used in the Ayurvedic, Unani, Sindh, and Traditional Chinese Medicine for the treatment of knee joint pain, lithiasis, diuresis, urinary disorder and swelling. In the present study, methanol extract of L. nodiflora showed promising xanthine oxidase (XOD) inhibitory activity in vitro. Bioactivity-guided fractionation of methanol extract yielded four fractions (F1-F4) with F3 being identified as the most potent fraction. Further purification of F3 afforded five bioactive compounds, including two phenylethanoid glycosides arenarioside (1) and verbascoside (2), and three flavonoids 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5), of which 1 and 4 were first time isolated from L. nodiflora. The methanol extract, fractions, and chemical constituents were then tested for potential antihyperuricemic activity in vivo using potassium oxonate- and hypoxanthine-induced hyperuricemic rat model. Oral treatment with methanol extract effectively and dose-dependently reduced the serum uric acid level of hyperuricemic rats. 2016-07 Thesis http://eprints.usm.my/32134/ http://eprints.usm.my/32134/1/CHENG_LEE_CHUEN_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Cheng, Lee Chuen Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity
description	Lippia nodiflora telah digunakan secara tradisional dalam Ayurveda, Unani, Sindh, dan Perubatan Tradisional Cina untuk rawatan sakit lutut sendi, lithiasis, diuresis, penyakit urinari dan bengkak. Dalam kajian ini, ekstrak metanol L. nodiflora menunjukkan aktiviti perencatan xantina oksidase (XOD) secara in vitro baik. Pemeringkatan berpandukan bioaktiviti ekstrak metanol menghasilkan empat pecahan (F1-F4) dengan F3 dikenal pasti sebagai pecahan yang paling poten. Penulenan F3 selanjutnya menghasilkan lima sebatian bioaktif, termasuk dua feniletanoid glikosida arenariosida (1) dan verbaskosida (2), dan tiga flavonoid 6-hidroksiluteolin (3), 6-hidroksiluteolin-7-O-glikosida (4), dan nodifloretin (5), di mana 1 dan 4 diasingkan untuk kali pertama daripada L. nodiflora. Ekstrak metanol, pecahan-pecahan, dan juzuk kimia tersebut kemudiannya diuji untuk activiti antihiperurisemik berpotensi secara in vivo dengan menggunakan model tikus hiperurisemik diaruh oleh kalium oxonate dan hipoksantina. Rawatan oral ekstrak metanol menurunkan paras asid urik serum tikus hiperurisemik dengan berkesan dan bersandarkan dos. Lippia nodiflora has been traditionally used in the Ayurvedic, Unani, Sindh, and Traditional Chinese Medicine for the treatment of knee joint pain, lithiasis, diuresis, urinary disorder and swelling. In the present study, methanol extract of L. nodiflora showed promising xanthine oxidase (XOD) inhibitory activity in vitro. Bioactivity-guided fractionation of methanol extract yielded four fractions (F1-F4) with F3 being identified as the most potent fraction. Further purification of F3 afforded five bioactive compounds, including two phenylethanoid glycosides arenarioside (1) and verbascoside (2), and three flavonoids 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5), of which 1 and 4 were first time isolated from L. nodiflora. The methanol extract, fractions, and chemical constituents were then tested for potential antihyperuricemic activity in vivo using potassium oxonate- and hypoxanthine-induced hyperuricemic rat model. Oral treatment with methanol extract effectively and dose-dependently reduced the serum uric acid level of hyperuricemic rats.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Cheng, Lee Chuen
author_facet	Cheng, Lee Chuen
author_sort	Cheng, Lee Chuen
title	Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity
title_short	Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity
title_full	Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity
title_fullStr	Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity
title_full_unstemmed	Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity
title_sort	phytochemical studies of lippia nodiflora (l.) michx and its anti-hyperuricemic activity
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
publishDate	2016
url	http://eprints.usm.my/32134/1/CHENG_LEE_CHUEN_24%28NN%29.pdf
_version_	1747820531916210176

Phytochemical Studies Of Lippia Nodiflora (L.) Michx And Its Anti-Hyperuricemic Activity

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