Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue

Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue

Pengesanan beberapa jenis kanser boleh dilakukan dengan menggunakan antibodi monoklon (AbM). Kajian menunjukkan bahawa jenis-jenis kanser yang berbeza mempunyai protein tak normal yang tertentu yang boleh dikenal pasti oleh AbM. IBMR3 AbM dibangunkan daripada peptida sintetik yang berhubung denga...

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Main Author: Othman, Ekhlas Qaid Gazem
Format: Thesis
Language:English
Published: 2016
Subjects:
QH1 Natural history (General - Including nature conservation
geographical distribution)
Online Access:http://eprints.usm.my/32143/1/EKHLAS_QAID_GAZEM_24%28NN%29.pdf
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spelling my-usm-ep.321432019-04-12T05:25:14Z Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue 2016-09 Othman, Ekhlas Qaid Gazem QH1 Natural history (General - Including nature conservation, geographical distribution) Pengesanan beberapa jenis kanser boleh dilakukan dengan menggunakan antibodi monoklon (AbM). Kajian menunjukkan bahawa jenis-jenis kanser yang berbeza mempunyai protein tak normal yang tertentu yang boleh dikenal pasti oleh AbM. IBMR3 AbM dibangunkan daripada peptida sintetik yang berhubung dengan jujukan asid amino terpilih daripada reseptor interleukin-4 manusia. Kajian ini adalah untuk mengesan ekspresi IBMR3 antigen dalam karsinoma termasuk Manusia carcinoma berhubung dgn pangkal tekak epitelium (HEp-2), adenokarsinoma kolon Manusia (HT-29) dan tisu kanser payudara. Ungkapan IBMR3 antigen dikesan pada HEp-2 dan HT-29 menggunakan ujian berbentuk tidak langsung pewarnaan, aliran sitometri pewarnaan. Hasil kajian menunjukkan persatuan kemungkinan IBMR3 antigen ungkapan dalam karsinoma (kanser sel-sel epitelium) dengan pewarnaan cytoplasmic positif. HEp-2 dan HT-29 telah dipisahkan melalui 1-D immunoblot dan keputusan menunjukkan dua band dengan berat molekul (MWS) 45 dan 25 kDa dalam HT-29 dan satu band dengan MWS 25 kDa dalam HEp-2. IBMR3 antigen ungkapan itu dikesan pada 48 sampel kanser payudara Malaysia dan microarray (TMA) slaid tisu kanser payudara 430 dan 132 tisu payudara yang normal bersebelahan dengan kanser payudara melalui IHC. The detection of several types of cancer is possible by using monoclonal antibody (MAb). Studies have shown that different types of cancer have related specific abnormal proteins, which are identified by MAb. IBMR3 is a MAb which was raised against synthetic peptides that are associated with the selected amino acid sequence of human interleukin-4 receptors. This research is to detect the expression of IBMR3 antigen in carcinoma including Human laryngeal epithelial carcinoma (HEp-2), Human colon adenocarcinoma (HT-29) and breast cancer tissues. The expression of IBMR3 antigen was detected on HEp-2 and HT-29 using indirect immunofluorescent staining, flow cytometry staining. The results showed the possible association of IBMR3 antigen expression in carcinoma (epithelial cancer cells) with positive cytoplasmic staining. HEp-2 and HT-29 were separated via 1-D immunoblot and the results showed 2 bands with molecular weights (MWs) of 45 and 25 kDa in HT-29 and one band with MWs of 25 kDa in HEp-2. IBMR3 antigen expression was detected on 48 samples of Malaysian breast cancer and microarray (TMA) slides of 430 breast cancer tissues and 132 normal breast tissues adjacent to breast cancer through IHC. 2016-09 Thesis http://eprints.usm.my/32143/ http://eprints.usm.my/32143/1/EKHLAS_QAID_GAZEM_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1 Natural history (General - Including nature conservation
geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation
geographical distribution)
Othman, Ekhlas Qaid Gazem
Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue
description Pengesanan beberapa jenis kanser boleh dilakukan dengan menggunakan antibodi monoklon (AbM). Kajian menunjukkan bahawa jenis-jenis kanser yang berbeza mempunyai protein tak normal yang tertentu yang boleh dikenal pasti oleh AbM. IBMR3 AbM dibangunkan daripada peptida sintetik yang berhubung dengan jujukan asid amino terpilih daripada reseptor interleukin-4 manusia. Kajian ini adalah untuk mengesan ekspresi IBMR3 antigen dalam karsinoma termasuk Manusia carcinoma berhubung dgn pangkal tekak epitelium (HEp-2), adenokarsinoma kolon Manusia (HT-29) dan tisu kanser payudara. Ungkapan IBMR3 antigen dikesan pada HEp-2 dan HT-29 menggunakan ujian berbentuk tidak langsung pewarnaan, aliran sitometri pewarnaan. Hasil kajian menunjukkan persatuan kemungkinan IBMR3 antigen ungkapan dalam karsinoma (kanser sel-sel epitelium) dengan pewarnaan cytoplasmic positif. HEp-2 dan HT-29 telah dipisahkan melalui 1-D immunoblot dan keputusan menunjukkan dua band dengan berat molekul (MWS) 45 dan 25 kDa dalam HT-29 dan satu band dengan MWS 25 kDa dalam HEp-2. IBMR3 antigen ungkapan itu dikesan pada 48 sampel kanser payudara Malaysia dan microarray (TMA) slaid tisu kanser payudara 430 dan 132 tisu payudara yang normal bersebelahan dengan kanser payudara melalui IHC. The detection of several types of cancer is possible by using monoclonal antibody (MAb). Studies have shown that different types of cancer have related specific abnormal proteins, which are identified by MAb. IBMR3 is a MAb which was raised against synthetic peptides that are associated with the selected amino acid sequence of human interleukin-4 receptors. This research is to detect the expression of IBMR3 antigen in carcinoma including Human laryngeal epithelial carcinoma (HEp-2), Human colon adenocarcinoma (HT-29) and breast cancer tissues. The expression of IBMR3 antigen was detected on HEp-2 and HT-29 using indirect immunofluorescent staining, flow cytometry staining. The results showed the possible association of IBMR3 antigen expression in carcinoma (epithelial cancer cells) with positive cytoplasmic staining. HEp-2 and HT-29 were separated via 1-D immunoblot and the results showed 2 bands with molecular weights (MWs) of 45 and 25 kDa in HT-29 and one band with MWs of 25 kDa in HEp-2. IBMR3 antigen expression was detected on 48 samples of Malaysian breast cancer and microarray (TMA) slides of 430 breast cancer tissues and 132 normal breast tissues adjacent to breast cancer through IHC.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Othman, Ekhlas Qaid Gazem
author_facet Othman, Ekhlas Qaid Gazem
author_sort Othman, Ekhlas Qaid Gazem
title Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue
title_short Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue
title_full Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue
title_fullStr Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue
title_full_unstemmed Expression Of Monoclonal Antibody IBMR3 Antigen In Breast Cancer Tissue
title_sort expression of monoclonal antibody ibmr3 antigen in breast cancer tissue
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate 2016
url http://eprints.usm.my/32143/1/EKHLAS_QAID_GAZEM_24%28NN%29.pdf
_version_ 1747820533892775936

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