Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells

Kanser pangkal rahim masih kekal sebagai kanser yang kedua tertinggi di kalangan wanita dan punca ketiga utama kematian walaupun pelbagai rawatan sedia ada. Kurang keberkesanan rawatan tersebut telah menyebabkan para saintis mencari rawatan alternatif bagi penyakit ini. Strategi agen anti-prolife...

Full description

Saved in:

Bibliographic Details
Main Author:	Shaik Othuman, Faiqah Husna
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RK1-715 Dentistry
Online Access:	http://eprints.usm.my/32147/1/FAIQAH_HUSNA_SHAIK_OTHUMAN_24%28NN%29.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.32147
record_format	uketd_dc
spelling	my-usm-ep.321472019-04-12T05:25:14Z Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells 2016-09 Shaik Othuman, Faiqah Husna RK1-715 Dentistry Kanser pangkal rahim masih kekal sebagai kanser yang kedua tertinggi di kalangan wanita dan punca ketiga utama kematian walaupun pelbagai rawatan sedia ada. Kurang keberkesanan rawatan tersebut telah menyebabkan para saintis mencari rawatan alternatif bagi penyakit ini. Strategi agen anti-proliferatif baru untuk kanser pangkal rahim adalah salah satu inovasi yang penting dalam melawan penyakit yang sengsara ini. Walaupun derivatif hydrazone sintetik telah digunakan secara meluas dalam penyelidikan kanser tetapi mekanisme anti-kanser yang sebenarnya yang ditunjukkan oleh bahan kimia ini masih belum diketahui. Kajian ini cuba menjelaskan kesan-kesan anti-proliferatif1 - [( Bromomethyl ) ( phenyl ) methyl ] -2- (2,4- dinitrophenyl ) hidrazin (C14H11BrN4O4) derivatif (kompaun Hydrazone) pada sel-sel kanser pangkal rahim melalui penentuan kepekatan perencatan (IC50), assay anti- proliferatif, assay apoptosis, assay kitaran sel, analisis Western Blot, assay invasi dan analisis cytotoxicity .Hasil kajian menunjukkan dos IC50 bagi kompaun Hydrazone adalah 0.03mg/ml pada sel-sel kanser pangkal rahim dan berjaya menghalang proliferasi sel-sel kanser berbanding dengan sel-sel kanser yang tidak dirawat. Di samping itu, aliran sitometri analisis mengesahkan hydrazone derivatif menyebabkan pembantutan sintesis deoxyribonucleic acid (DNA) pada fasa S dan G2 / M tanpa induksi apoptosis. Analisa ‘ western blot’ menunjukkan peningkatan ekspresi protein p27kip1 dan PCNA serta merencatkan ekspresi protein Cyclin E2 dan cdc25. Kesan anti-metastasis dikaji melalui analisis invasi dan ia menunjukkan kompaun hydrazone telah menghalang 50.94 % invasi sel HeLa. Kesimpulannya kompaun hydrazone memiliki kesan anti-proliferatif pada sel HeLa. Cervical cancer remains as the second most common type of cancer in women and it is the third leading cause of death despite various treatments that are available. Inefficiency of present treatments had triggered scientists to seek for an alternative treatment for this disease. Development of new anti-proliferative agent for cervical cancer is an important innovation in fighting this miserable disease. Although, synthetic hydrazone derivatives are widely being used in cancer research, the actual anticancer mechanism shown by these chemicals is still not yet understood. Therefore this study aimed to elucidate the anti-proliferative effects of the 1-[(Bromomethyl)(phenyl)methyl] -2-(2,4 -dinitrophenyl) hydrazine (C14H11BrN4O4) derivative (Hydrazone compound) on cervical cancer cells through determination of inhibitory concentration (IC50), anti-proliferative assay, apoptosis assay, cell cycle assay, western blot analysis, invasion assay and cytotoxicity analysis. The results showed the IC50 of the hydrazone compound at 0.03mg/ml on the cervical cancer cells and inhibits the proliferation of cervical cancer cells in a dose and time-dependent manner compared to the untreated control. In addition, the flow cytometry analysis confirmed the hydrazone derivative causes S and G2/M phase cell cycle arrest without induction of apoptosis. Western blot analysis showed that hydrazone compound had increased the p27kip1 and PCNA and suppressed Cyclin E2 and cdc25 protein expression. The anti-metastatic effect was studied through invasion assay and it showed the hydrazone compound had inhibited 50.94% of HeLa cells invasion. It is concluded the hydrazone compound possesses anti-proliferative effect on HeLa cells. 2016-09 Thesis http://eprints.usm.my/32147/ http://eprints.usm.my/32147/1/FAIQAH_HUSNA_SHAIK_OTHUMAN_24%28NN%29.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK1-715 Dentistry
spellingShingle	RK1-715 Dentistry Shaik Othuman, Faiqah Husna Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells
description	Kanser pangkal rahim masih kekal sebagai kanser yang kedua tertinggi di kalangan wanita dan punca ketiga utama kematian walaupun pelbagai rawatan sedia ada. Kurang keberkesanan rawatan tersebut telah menyebabkan para saintis mencari rawatan alternatif bagi penyakit ini. Strategi agen anti-proliferatif baru untuk kanser pangkal rahim adalah salah satu inovasi yang penting dalam melawan penyakit yang sengsara ini. Walaupun derivatif hydrazone sintetik telah digunakan secara meluas dalam penyelidikan kanser tetapi mekanisme anti-kanser yang sebenarnya yang ditunjukkan oleh bahan kimia ini masih belum diketahui. Kajian ini cuba menjelaskan kesan-kesan anti-proliferatif1 - [( Bromomethyl ) ( phenyl ) methyl ] -2- (2,4- dinitrophenyl ) hidrazin (C14H11BrN4O4) derivatif (kompaun Hydrazone) pada sel-sel kanser pangkal rahim melalui penentuan kepekatan perencatan (IC50), assay anti- proliferatif, assay apoptosis, assay kitaran sel, analisis Western Blot, assay invasi dan analisis cytotoxicity .Hasil kajian menunjukkan dos IC50 bagi kompaun Hydrazone adalah 0.03mg/ml pada sel-sel kanser pangkal rahim dan berjaya menghalang proliferasi sel-sel kanser berbanding dengan sel-sel kanser yang tidak dirawat. Di samping itu, aliran sitometri analisis mengesahkan hydrazone derivatif menyebabkan pembantutan sintesis deoxyribonucleic acid (DNA) pada fasa S dan G2 / M tanpa induksi apoptosis. Analisa ‘ western blot’ menunjukkan peningkatan ekspresi protein p27kip1 dan PCNA serta merencatkan ekspresi protein Cyclin E2 dan cdc25. Kesan anti-metastasis dikaji melalui analisis invasi dan ia menunjukkan kompaun hydrazone telah menghalang 50.94 % invasi sel HeLa. Kesimpulannya kompaun hydrazone memiliki kesan anti-proliferatif pada sel HeLa. Cervical cancer remains as the second most common type of cancer in women and it is the third leading cause of death despite various treatments that are available. Inefficiency of present treatments had triggered scientists to seek for an alternative treatment for this disease. Development of new anti-proliferative agent for cervical cancer is an important innovation in fighting this miserable disease. Although, synthetic hydrazone derivatives are widely being used in cancer research, the actual anticancer mechanism shown by these chemicals is still not yet understood. Therefore this study aimed to elucidate the anti-proliferative effects of the 1-[(Bromomethyl)(phenyl)methyl] -2-(2,4 -dinitrophenyl) hydrazine (C14H11BrN4O4) derivative (Hydrazone compound) on cervical cancer cells through determination of inhibitory concentration (IC50), anti-proliferative assay, apoptosis assay, cell cycle assay, western blot analysis, invasion assay and cytotoxicity analysis. The results showed the IC50 of the hydrazone compound at 0.03mg/ml on the cervical cancer cells and inhibits the proliferation of cervical cancer cells in a dose and time-dependent manner compared to the untreated control. In addition, the flow cytometry analysis confirmed the hydrazone derivative causes S and G2/M phase cell cycle arrest without induction of apoptosis. Western blot analysis showed that hydrazone compound had increased the p27kip1 and PCNA and suppressed Cyclin E2 and cdc25 protein expression. The anti-metastatic effect was studied through invasion assay and it showed the hydrazone compound had inhibited 50.94% of HeLa cells invasion. It is concluded the hydrazone compound possesses anti-proliferative effect on HeLa cells.
format	Thesis
qualification_level	Master's degree
author	Shaik Othuman, Faiqah Husna
author_facet	Shaik Othuman, Faiqah Husna
author_sort	Shaik Othuman, Faiqah Husna
title	Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells
title_short	Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells
title_full	Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells
title_fullStr	Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells
title_full_unstemmed	Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells
title_sort	investigation of the anti-proliferative effect of synthetic 1-[(bromomethyl)(phenyl) methyl]-2-(2,4-dinitrophenyl)hydrazine derivative on cervical cancer cells
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute)
publishDate	2016
url	http://eprints.usm.my/32147/1/FAIQAH_HUSNA_SHAIK_OTHUMAN_24%28NN%29.pdf
_version_	1747820534381412352

Investigation Of The Anti-Proliferative Effect Of Synthetic 1-[(Bromomethyl)(Phenyl) Methyl]-2-(2,4-Dinitrophenyl)Hydrazine Derivative On Cervical Cancer Cells

Similar Items