Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer

Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer

Teh Orthosiphon stamineus Benth. (Lamiaceae) digunakan secara meluas dalam perubatan tradisional. Kajian terbaru menunjukkan bahawa 50% ekstrak ethanolik daripada Orthosiphon stamineus (EOS) dan sebatian aktif, asid rosmarinik (RA), memaparkan kesan-kesan anti-angiogenik, anti-radang dan anti-tumor...

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Main Author: Al-Suede, Fouad Saleih Resq
Format: Thesis
Language:English
Published: 2016
Subjects:
RS1-441 Pharmacy and materia medica
Online Access:http://eprints.usm.my/32154/1/FOUAD_SALEIH_RESQ_AL-SUEDE_24%28NN%29.pdf
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spelling my-usm-ep.321542019-04-12T05:25:12Z Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer 2016-09 Al-Suede, Fouad Saleih Resq RS1-441 Pharmacy and materia medica Teh Orthosiphon stamineus Benth. (Lamiaceae) digunakan secara meluas dalam perubatan tradisional. Kajian terbaru menunjukkan bahawa 50% ekstrak ethanolik daripada Orthosiphon stamineus (EOS) dan sebatian aktif, asid rosmarinik (RA), memaparkan kesan-kesan anti-angiogenik, anti-radang dan anti-tumor yang ketara dalam pelbagai model eksperimen. Walau bagaimanapun, mekanisme yang mendasari sifat-sifat ini tidak dinilai dengan sepenuhnya. Kajian yang dijalankan ini bertujuan untuk menilaikan lagi mekanisme molekul yang mendasari anti-tumor dan anti-angiogenik. Dalam model eksperimen penghijrahan, perkembangan dan pembentukan tiub, cerakin kedua-dua EOS dan RA aktif menyebabkan perencatan ketara terhadap fungsi sel endothelial manusia (HUVECs) yang penting bagi merangsang proses angiogenesis. Dalam kedua-dua kajian in vitro dan in vivo, penindasan besar neovaskularisasi dalam model aorta tikus, CAM dan plug matrigel juga diperhatikan. Kajian cerakin multipleks menunjukkan pengurangan faktor pertumbuhan utama bagi lata pro-angiogenik dan perkembangan tumor iaitu faktor pertumbuhan endothelial vaskular (VEGF), faktor pertumbuhan fibroblast asas (b-FGF), transformasi faktor pertumbuhan transformasi (TGF-α), faktor nekrosis tumor (TNF-β) dan interleukin-1, 2, 7. Orthosiphon stamineus Benth. (Lamiaceae) tea is widely consumed traditionally for its vast medicinal value. Recent studies revealed that 50% ethanolic extract of Orthosiphon stamineus (EOS) and its active compound, rosmarinic acid (RA), displayed significant anti-angiogenic, anti-inflammatory and anti-tumor effects in various experimental models. However, the mechanisms underlying these properties have not been fully evaluated. The present work aims to further evaluate the molecular mechanisms underlying its anti-tumour and anti-angiogenic properties. In migration, proliferation and tube formation assay, both EOS and its active RA caused significant inhibition of human endothelial cell (HUVECs) functions crucial for promotion of angiogenesis. Both in vitro and in vivo studies revealed significant suppression of neovascularisation in rat aortic ring, CAM and matrigel plug. Multiplex array studies showed reduction of key growth factors for pro-angiogenic cascade and tumor development i.e. Vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b-FGF), transforming growth factor alpha (TGF-α), tumor necrosis factor (TNF-β) and interleukin-1, 2, 7. 2016-09 Thesis http://eprints.usm.my/32154/ http://eprints.usm.my/32154/1/FOUAD_SALEIH_RESQ_AL-SUEDE_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RS1-441 Pharmacy and materia medica
spellingShingle RS1-441 Pharmacy and materia medica
Al-Suede, Fouad Saleih Resq
Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer
description Teh Orthosiphon stamineus Benth. (Lamiaceae) digunakan secara meluas dalam perubatan tradisional. Kajian terbaru menunjukkan bahawa 50% ekstrak ethanolik daripada Orthosiphon stamineus (EOS) dan sebatian aktif, asid rosmarinik (RA), memaparkan kesan-kesan anti-angiogenik, anti-radang dan anti-tumor yang ketara dalam pelbagai model eksperimen. Walau bagaimanapun, mekanisme yang mendasari sifat-sifat ini tidak dinilai dengan sepenuhnya. Kajian yang dijalankan ini bertujuan untuk menilaikan lagi mekanisme molekul yang mendasari anti-tumor dan anti-angiogenik. Dalam model eksperimen penghijrahan, perkembangan dan pembentukan tiub, cerakin kedua-dua EOS dan RA aktif menyebabkan perencatan ketara terhadap fungsi sel endothelial manusia (HUVECs) yang penting bagi merangsang proses angiogenesis. Dalam kedua-dua kajian in vitro dan in vivo, penindasan besar neovaskularisasi dalam model aorta tikus, CAM dan plug matrigel juga diperhatikan. Kajian cerakin multipleks menunjukkan pengurangan faktor pertumbuhan utama bagi lata pro-angiogenik dan perkembangan tumor iaitu faktor pertumbuhan endothelial vaskular (VEGF), faktor pertumbuhan fibroblast asas (b-FGF), transformasi faktor pertumbuhan transformasi (TGF-α), faktor nekrosis tumor (TNF-β) dan interleukin-1, 2, 7. Orthosiphon stamineus Benth. (Lamiaceae) tea is widely consumed traditionally for its vast medicinal value. Recent studies revealed that 50% ethanolic extract of Orthosiphon stamineus (EOS) and its active compound, rosmarinic acid (RA), displayed significant anti-angiogenic, anti-inflammatory and anti-tumor effects in various experimental models. However, the mechanisms underlying these properties have not been fully evaluated. The present work aims to further evaluate the molecular mechanisms underlying its anti-tumour and anti-angiogenic properties. In migration, proliferation and tube formation assay, both EOS and its active RA caused significant inhibition of human endothelial cell (HUVECs) functions crucial for promotion of angiogenesis. Both in vitro and in vivo studies revealed significant suppression of neovascularisation in rat aortic ring, CAM and matrigel plug. Multiplex array studies showed reduction of key growth factors for pro-angiogenic cascade and tumor development i.e. Vascular endothelial growth factor (VEGF), basic fibroblast growth factor (b-FGF), transforming growth factor alpha (TGF-α), tumor necrosis factor (TNF-β) and interleukin-1, 2, 7.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Al-Suede, Fouad Saleih Resq
author_facet Al-Suede, Fouad Saleih Resq
author_sort Al-Suede, Fouad Saleih Resq
title Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer
title_short Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer
title_full Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer
title_fullStr Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer
title_full_unstemmed Investigation Of Molecular Mechanisms Underlying The Anti-Tumor And Anti-Angiogenic Activities Of Orthosiphon Stamineus Towards Colorectal Cancer
title_sort investigation of molecular mechanisms underlying the anti-tumor and anti-angiogenic activities of orthosiphon stamineus towards colorectal cancer
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Farmasi (School of Pharmaceutical Sciences)
publishDate 2016
url http://eprints.usm.my/32154/1/FOUAD_SALEIH_RESQ_AL-SUEDE_24%28NN%29.pdf
_version_ 1747820535849418752

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