Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna

Kajian ini dijalankan untuk mengkaji keberkesanan larva peringkat 3 Lucilia cuprina Wiedemann (1830) dalam analisis toksikologi untuk mengesan metabolit heroin dengan menggunakan aplikasi GCMS dan dalam analisis molekul dengan menggunakan Cytochrome b gen (mtDNA) untuk mengenalpasti jujukan DNA dagi...

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Main Author:	Ishak, Nooratiny
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/32231/1/NOORATINY_ISHAK_24%28NN%29.pdf
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id	my-usm-ep.32231
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spelling	my-usm-ep.322312019-04-12T05:25:15Z Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna 2016-08 Ishak, Nooratiny QH1 Natural history (General - Including nature conservation, geographical distribution) Kajian ini dijalankan untuk mengkaji keberkesanan larva peringkat 3 Lucilia cuprina Wiedemann (1830) dalam analisis toksikologi untuk mengesan metabolit heroin dengan menggunakan aplikasi GCMS dan dalam analisis molekul dengan menggunakan Cytochrome b gen (mtDNA) untuk mengenalpasti jujukan DNA daging kerbau cincang (yang telah digunakan sebagai bahan eksperimen dalam kajian ini) tanpa heroin. Kajian yang dilakukan juga menyiasat kesan heroin kepada tempoh kitaran hidup Lucilia cuprina. Selepas itu kaedah yang dibangunkan ini digunakan dalam kes-kes forensik untuk mengkaji keberkesanan kaedah tersebut dalam mengendalikan kes-kes forensik manusia dan haiwan. Analisis toksikologi yang dilakukan ke atas larva peringkat 3 telah memberi keputusan morfin, iaitu metabolit heroin. Heroin yang telah dicampurkan bersama dengan daging kerbau cincang (Bubalus bubalis, Linnaeus, 1758) di dalam kepekatan yang berbeza telah mempercepatkan pertumbuhan larva L. cuprina berbanding dengan larva pada daging kerbau cincang tanpa heroin yang mempunyai pertumbuhan yang lebih panjang. Analisis molekul yang dijalankan pada sistem usus larva peringkat 3, berjaya mengamplifikasi jujukan DNA daging kerbau cincang iaitu Bubalus bubalis. This study investigates the capability of 3rd instar larvae of Lucilia cuprina Wiedemann (1830) in toxicology analysis to detect the heroin metabolites by using GCMS application and in molecular analysis by utilizing the Cytochrome b gene (mtDNA) for precise identification of minced buffalo meat (which had been used as experimental materials in this study) in the absence of heroin. The study conducted also investigates the effect of heroin on the Lucilia cuprina duration of life cycle. Then the developed method was applied in exact forensic cases to examine the effectiveness of the developed method in handling both human and animal forensic cases. The toxicology analysis conducted on the 3rd instar larval crop was successfully detecting a metabolite of heroin, such as morphine. The heroin that was mixed with the minced buffalo (Bubalus bubalis, Linnaeus, 1758) meat in different concentrations, accelerated the duration of life cycle of L. cuprina larvae compared to the larvae that were collected from the minced buffalo meat without heroin, which had a longer life cycle. The molecular analysis conducted on 3rd instar larval crop, succesfully amplify desired results of minced buffalo meat (with heroin) DNA sequence, which is Bubalus bubalis. 2016-08 Thesis http://eprints.usm.my/32231/ http://eprints.usm.my/32231/1/NOORATINY_ISHAK_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Ishak, Nooratiny Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna
description	Kajian ini dijalankan untuk mengkaji keberkesanan larva peringkat 3 Lucilia cuprina Wiedemann (1830) dalam analisis toksikologi untuk mengesan metabolit heroin dengan menggunakan aplikasi GCMS dan dalam analisis molekul dengan menggunakan Cytochrome b gen (mtDNA) untuk mengenalpasti jujukan DNA daging kerbau cincang (yang telah digunakan sebagai bahan eksperimen dalam kajian ini) tanpa heroin. Kajian yang dilakukan juga menyiasat kesan heroin kepada tempoh kitaran hidup Lucilia cuprina. Selepas itu kaedah yang dibangunkan ini digunakan dalam kes-kes forensik untuk mengkaji keberkesanan kaedah tersebut dalam mengendalikan kes-kes forensik manusia dan haiwan. Analisis toksikologi yang dilakukan ke atas larva peringkat 3 telah memberi keputusan morfin, iaitu metabolit heroin. Heroin yang telah dicampurkan bersama dengan daging kerbau cincang (Bubalus bubalis, Linnaeus, 1758) di dalam kepekatan yang berbeza telah mempercepatkan pertumbuhan larva L. cuprina berbanding dengan larva pada daging kerbau cincang tanpa heroin yang mempunyai pertumbuhan yang lebih panjang. Analisis molekul yang dijalankan pada sistem usus larva peringkat 3, berjaya mengamplifikasi jujukan DNA daging kerbau cincang iaitu Bubalus bubalis. This study investigates the capability of 3rd instar larvae of Lucilia cuprina Wiedemann (1830) in toxicology analysis to detect the heroin metabolites by using GCMS application and in molecular analysis by utilizing the Cytochrome b gene (mtDNA) for precise identification of minced buffalo meat (which had been used as experimental materials in this study) in the absence of heroin. The study conducted also investigates the effect of heroin on the Lucilia cuprina duration of life cycle. Then the developed method was applied in exact forensic cases to examine the effectiveness of the developed method in handling both human and animal forensic cases. The toxicology analysis conducted on the 3rd instar larval crop was successfully detecting a metabolite of heroin, such as morphine. The heroin that was mixed with the minced buffalo (Bubalus bubalis, Linnaeus, 1758) meat in different concentrations, accelerated the duration of life cycle of L. cuprina larvae compared to the larvae that were collected from the minced buffalo meat without heroin, which had a longer life cycle. The molecular analysis conducted on 3rd instar larval crop, succesfully amplify desired results of minced buffalo meat (with heroin) DNA sequence, which is Bubalus bubalis.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ishak, Nooratiny
author_facet	Ishak, Nooratiny
author_sort	Ishak, Nooratiny
title	Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna
title_short	Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna
title_full	Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna
title_fullStr	Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna
title_full_unstemmed	Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna
title_sort	use of lucilia cuprina wiedemann (1830) larvae for detection of heroin metabolites and identification of host dna
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/32231/1/NOORATINY_ISHAK_24%28NN%29.pdf
_version_	1747820549080350720

Use Of Lucilia Cuprina Wiedemann (1830) Larvae For Detection Of Heroin Metabolites And Identification Of Host Dna

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