An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes

Dalam usaha untuk membangunkan lignin tandan kosong kelapa sawit (TKKS) sebagai makanan tambahan dalam nutraseutikal dan kesihatan, penyelidikan tentang potensinya dalam berinteraksi dengan drug lain melalui perencatan atau peningkatan enzim metabolisme drug (DME) mampu memastikan keselamatan produk...

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Main Author:	Mohd Salleh, Norliyana
Format:	Thesis
Language:	English
Published:	2016
Subjects:	RM300-666 Drugs and their actions
Online Access:	http://eprints.usm.my/32234/1/NORLIYANA_MOHD_SALLEH_24%28NN%29.pdf
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id	my-usm-ep.32234
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spelling	my-usm-ep.322342019-04-12T05:25:14Z An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes 2016-09 Mohd Salleh, Norliyana RM300-666 Drugs and their actions Dalam usaha untuk membangunkan lignin tandan kosong kelapa sawit (TKKS) sebagai makanan tambahan dalam nutraseutikal dan kesihatan, penyelidikan tentang potensinya dalam berinteraksi dengan drug lain melalui perencatan atau peningkatan enzim metabolisme drug (DME) mampu memastikan keselamatan produk. Oleh itu, kajian ini dijalankan untuk mengkaji kesan lignin TKKS dan sebatian-sebatian teroksida utamanya terhadap DME fasa II UDP-glukuronosiltransferase (UGT) dalam mikrosom hati tikus (RLM) dan mikrosom ginjal tikus (RKM) serta glutation S-transferase (GST) dalam fraksi sitosol hati tikus (RLC) dan fraksi sitosol ginjal tikus (RKC) secara rawatan in vitro dan in vivo. Pencirian lignin TKKS menunjukkan bahawa ketiga-tiga jenis ekstrak lignin TKKS (soda, kraft dan organosolv) terdiri daripada siringil dan guaiasil. Jumlah kandungan flavonoid pada lignin TKKS meningkat dalam turutan organosolv < kraft < soda. Analisis kromatografi cecair berprestasi tinggi (HPLC) menunjukkan bahawa siringaldehid merupakan sebatian teroksida utama yang terdapat dalam lignin TKKS diikuti dengan vanilin dan p-hidroksibenzaldehid. lignin TKKS dinilai sebagai aktiviti penghapus radikal bebas DPPH yang berpotensi. In order to develop oil palm empty fruit bunch (EFB) lignin as a nutraceutical and health supplement, the investigation of its potential in interacting with other drugs via inhibition or induction of drug metabolizing enzymes (DME) would contribute towards product safety. Therefore, this study was carried out to investigate the effect of oil palm EFB lignin and its main oxidative compounds on phase II DME, UDP-glucuronosyltranferases (UGT) in rat liver microsomes (RLM) and rat kidney microsomes (RKM) as well as glutathione-S-transferases (GST) in rat liver cytosolic fraction (RLC) and rat kidney cytosolic fraction (RKC) by in vitro and in vivo treatment. The characterization of oil palm EFB lignin showed that all three types of oil palm EFB lignin extracts (soda, kraft and organosolv) consist of syringyl and guaiacyl. The total flavonoids content of oil palm EFB lignin was increased in the order of organosolv < kraft < soda. The high performance liquid chromatography (HPLC) analysis revealed that syringaldehyde was the main oxidation compound in oil palm EFB lignin followed by vanillin and p-hydroxybenzaldehyde. The oil palm EFB lignin evaluated as potential DPPH-radical scavenging activity. 2016-09 Thesis http://eprints.usm.my/32234/ http://eprints.usm.my/32234/1/NORLIYANA_MOHD_SALLEH_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Penyelidikan Dadah dan Ubat-Ubatan (Centre for Drug Research)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RM300-666 Drugs and their actions
spellingShingle	RM300-666 Drugs and their actions Mohd Salleh, Norliyana An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes
description	Dalam usaha untuk membangunkan lignin tandan kosong kelapa sawit (TKKS) sebagai makanan tambahan dalam nutraseutikal dan kesihatan, penyelidikan tentang potensinya dalam berinteraksi dengan drug lain melalui perencatan atau peningkatan enzim metabolisme drug (DME) mampu memastikan keselamatan produk. Oleh itu, kajian ini dijalankan untuk mengkaji kesan lignin TKKS dan sebatian-sebatian teroksida utamanya terhadap DME fasa II UDP-glukuronosiltransferase (UGT) dalam mikrosom hati tikus (RLM) dan mikrosom ginjal tikus (RKM) serta glutation S-transferase (GST) dalam fraksi sitosol hati tikus (RLC) dan fraksi sitosol ginjal tikus (RKC) secara rawatan in vitro dan in vivo. Pencirian lignin TKKS menunjukkan bahawa ketiga-tiga jenis ekstrak lignin TKKS (soda, kraft dan organosolv) terdiri daripada siringil dan guaiasil. Jumlah kandungan flavonoid pada lignin TKKS meningkat dalam turutan organosolv < kraft < soda. Analisis kromatografi cecair berprestasi tinggi (HPLC) menunjukkan bahawa siringaldehid merupakan sebatian teroksida utama yang terdapat dalam lignin TKKS diikuti dengan vanilin dan p-hidroksibenzaldehid. lignin TKKS dinilai sebagai aktiviti penghapus radikal bebas DPPH yang berpotensi. In order to develop oil palm empty fruit bunch (EFB) lignin as a nutraceutical and health supplement, the investigation of its potential in interacting with other drugs via inhibition or induction of drug metabolizing enzymes (DME) would contribute towards product safety. Therefore, this study was carried out to investigate the effect of oil palm EFB lignin and its main oxidative compounds on phase II DME, UDP-glucuronosyltranferases (UGT) in rat liver microsomes (RLM) and rat kidney microsomes (RKM) as well as glutathione-S-transferases (GST) in rat liver cytosolic fraction (RLC) and rat kidney cytosolic fraction (RKC) by in vitro and in vivo treatment. The characterization of oil palm EFB lignin showed that all three types of oil palm EFB lignin extracts (soda, kraft and organosolv) consist of syringyl and guaiacyl. The total flavonoids content of oil palm EFB lignin was increased in the order of organosolv < kraft < soda. The high performance liquid chromatography (HPLC) analysis revealed that syringaldehyde was the main oxidation compound in oil palm EFB lignin followed by vanillin and p-hydroxybenzaldehyde. The oil palm EFB lignin evaluated as potential DPPH-radical scavenging activity.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Mohd Salleh, Norliyana
author_facet	Mohd Salleh, Norliyana
author_sort	Mohd Salleh, Norliyana
title	An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes
title_short	An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes
title_full	An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes
title_fullStr	An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes
title_full_unstemmed	An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes
title_sort	evaluation of oil palm empty fruit bunch lignin on selected phase ii drug metabolizing enzymes
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Penyelidikan Dadah dan Ubat-Ubatan (Centre for Drug Research)
publishDate	2016
url	http://eprints.usm.my/32234/1/NORLIYANA_MOHD_SALLEH_24%28NN%29.pdf
_version_	1747820549815402496

An Evaluation Of Oil Palm Empty Fruit Bunch Lignin On Selected Phase Ii Drug Metabolizing Enzymes

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