Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations

Penghibridan semulajadi telah diyakini kerapkali berlaku pada Cryptocoryne Wydler dan dianggap sebagai sumber kepada kerumitan taksonomi pada genus ini. Penyelidikan ini melibatkan gabungan kajian daripada data jujukan DNA (kawasan tertranskripsi dalaman (ITS) DNA nuklear ribosom dan gen matK daripa...

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Main Author:	Rusly, Rosazlina
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/32253/1/ROSAZLINA_RUSLY_24%28NN%29.pdf
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spelling	my-usm-ep.322532019-04-12T05:25:19Z Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations 2016-07 Rusly, Rosazlina QH1 Natural history (General - Including nature conservation, geographical distribution) Penghibridan semulajadi telah diyakini kerapkali berlaku pada Cryptocoryne Wydler dan dianggap sebagai sumber kepada kerumitan taksonomi pada genus ini. Penyelidikan ini melibatkan gabungan kajian daripada data jujukan DNA (kawasan tertranskripsi dalaman (ITS) DNA nuklear ribosom dan gen matK daripada DNA kloroplas) untuk mengenalpasti induk kepada hibrid putatif Cryptocoryne daripada Semenanjung Malaysia. Berdasarkan kepada ciri-ciri morfologi tumbuhan ini dikenalpasti secara tentatif sebagai Cryptocoryne ×purpurea Ridl. nothovar. purpurea; tumbuhan steril yang telah lama dianggap sebagai hibrid, kemungkinan daripada dua spesies yang berkaitan; Cryptocoryne cordata Griff. var. cordata dan Cryptocoryne griffithii Schott. Status hibrid dan induk-induk ini dibuktikan secara bebas dengan kehadiran pada individu hibrid corak jujukan ITS daripada kedua-dua spesies induk ini. Tumbuhan hibrid ini berkongsi persamaan jujukan matK daripada C. cordata var. cordata dan C. griffithii, menunjukkan kedua-dua spesies induk putatif ini telah menjadi induk betina. Penghibridan timbal balik di antara kedua-dua spesies ini dilihat sebagai simetri dan bukan satu arah. Kajian ini juga bertujuan untuk membangunkan penanda mikrosatelit menggunakan jujukan generasi (Roche 454 pyrosequencing) daripada DNA genomik C. cordata var. cordata. Natural hybridization has been confirmed to occur frequently in Cryptocoryne Wydler and considered a source of taxonomic complexity in this genus. This research involved a combined study of DNA sequencing data (internal transcribed spacer (ITS) of nuclear ribosomal DNA and matK gene of chloroplast DNA) to identify the parentage of a putative Cryptocoryne hybrid from Peninsular Malaysia. Based on the morphological characters the plant was tentatively identified as Cryptocoryne ×purpurea Ridl. nothovar. purpurea; a sterile plant which has long been considered a hybrid, possibly from two related species; Cryptocoryne cordata Griff. var. cordata and Cryptocoryne griffithii Schott. The hybrid status and its putative parents was independently confirmed by the presence in hybrid individuals of an additive ITS sequence pattern from these two parental species. The hybrid plants shared the identical matK sequences from C. cordata var. cordata and C. griffithii, which indicated that both putative parental species had functioned as the maternal parent. Reciprocal hybridization between the two species seems to be symmetrical rather than unidirectional. This study also aimed at developing microsatellite markers using next generation sequencing (Roche 454 pyrosequencing) from the genomic DNA of C. cordata var. cordata. 2016-07 Thesis http://eprints.usm.my/32253/ http://eprints.usm.my/32253/1/ROSAZLINA_RUSLY_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Rusly, Rosazlina Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations
description	Penghibridan semulajadi telah diyakini kerapkali berlaku pada Cryptocoryne Wydler dan dianggap sebagai sumber kepada kerumitan taksonomi pada genus ini. Penyelidikan ini melibatkan gabungan kajian daripada data jujukan DNA (kawasan tertranskripsi dalaman (ITS) DNA nuklear ribosom dan gen matK daripada DNA kloroplas) untuk mengenalpasti induk kepada hibrid putatif Cryptocoryne daripada Semenanjung Malaysia. Berdasarkan kepada ciri-ciri morfologi tumbuhan ini dikenalpasti secara tentatif sebagai Cryptocoryne ×purpurea Ridl. nothovar. purpurea; tumbuhan steril yang telah lama dianggap sebagai hibrid, kemungkinan daripada dua spesies yang berkaitan; Cryptocoryne cordata Griff. var. cordata dan Cryptocoryne griffithii Schott. Status hibrid dan induk-induk ini dibuktikan secara bebas dengan kehadiran pada individu hibrid corak jujukan ITS daripada kedua-dua spesies induk ini. Tumbuhan hibrid ini berkongsi persamaan jujukan matK daripada C. cordata var. cordata dan C. griffithii, menunjukkan kedua-dua spesies induk putatif ini telah menjadi induk betina. Penghibridan timbal balik di antara kedua-dua spesies ini dilihat sebagai simetri dan bukan satu arah. Kajian ini juga bertujuan untuk membangunkan penanda mikrosatelit menggunakan jujukan generasi (Roche 454 pyrosequencing) daripada DNA genomik C. cordata var. cordata. Natural hybridization has been confirmed to occur frequently in Cryptocoryne Wydler and considered a source of taxonomic complexity in this genus. This research involved a combined study of DNA sequencing data (internal transcribed spacer (ITS) of nuclear ribosomal DNA and matK gene of chloroplast DNA) to identify the parentage of a putative Cryptocoryne hybrid from Peninsular Malaysia. Based on the morphological characters the plant was tentatively identified as Cryptocoryne ×purpurea Ridl. nothovar. purpurea; a sterile plant which has long been considered a hybrid, possibly from two related species; Cryptocoryne cordata Griff. var. cordata and Cryptocoryne griffithii Schott. The hybrid status and its putative parents was independently confirmed by the presence in hybrid individuals of an additive ITS sequence pattern from these two parental species. The hybrid plants shared the identical matK sequences from C. cordata var. cordata and C. griffithii, which indicated that both putative parental species had functioned as the maternal parent. Reciprocal hybridization between the two species seems to be symmetrical rather than unidirectional. This study also aimed at developing microsatellite markers using next generation sequencing (Roche 454 pyrosequencing) from the genomic DNA of C. cordata var. cordata.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Rusly, Rosazlina
author_facet	Rusly, Rosazlina
author_sort	Rusly, Rosazlina
title	Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations
title_short	Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations
title_full	Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations
title_fullStr	Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations
title_full_unstemmed	Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations
title_sort	molecular dna markers in parentage identification and clonal genetic structure of cryptocoryne ×purpurea ridl. nothovar. purpurea hybrid populations
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat (School of Biological Sciences)
publishDate	2016
url	http://eprints.usm.my/32253/1/ROSAZLINA_RUSLY_24%28NN%29.pdf
_version_	1747820553740222464

Molecular Dna Markers In Parentage Identification And Clonal Genetic Structure Of Cryptocoryne ×purpurea Ridl. nothovar. purpurea Hybrid Populations

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