Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra

Sejak kebelakangan ini, lipase daripada mikroorganisma termofilik telah menjadi tumpuan yang istimewa disebabkan kepelbagaian aplikasinya dalam sektor industri. Ini disebabkan oleh cirinya yang mempunyai kestabilan yang tinggi pada suhu yang tinggi dan tahan penyahaslian oleh bahan kimia. Geobacillu...

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Main Author:	Balan, Anuradha
Format:	Thesis
Language:	English
Published:	2015
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/32270/1/ANURADHA_BALAN.pdf
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id	my-usm-ep.32270
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spelling	my-usm-ep.322702019-04-12T05:25:44Z Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra 2015-04 Balan, Anuradha QH1 Natural history (General - Including nature conservation, geographical distribution) Sejak kebelakangan ini, lipase daripada mikroorganisma termofilik telah menjadi tumpuan yang istimewa disebabkan kepelbagaian aplikasinya dalam sektor industri. Ini disebabkan oleh cirinya yang mempunyai kestabilan yang tinggi pada suhu yang tinggi dan tahan penyahaslian oleh bahan kimia. Geobacillus thermodenitrificans IBRL-nra yang digunakan dalam penyelidikan ini telah dipencilkan dari kolam air panas di Labok, Kelantan, Malaysia dan mempunyai suhu pertumbuhan antara 45ºC ke 70ºC. Penghasilan lipase stabil haba daripada G. thermodenitrificans IBRL-nra pada suhu 65˚C telah dikaji secara kualitatif diatas plat agar penyaringan lipase. Penghasilan lipase stabil haba ekstrasel oleh G. thermodenitrificans IBRL-nra telah dijalankan di dalam sistem kelalang goncangan dan bioreaktor tangki teraduk 5L. Penghasilan lipase stabil haba dan pertumbuhan sel di dalam bioreaktor tangki teraduk telah meningkat sebanyak 5 kali ganda dan 3 kali ganda, masing-masing daripada penghasilannya di dalam sistem kelalang goncangan. Lipases from thermophiles have gained interest in recent years as they have various applications in industries. They play significant roles in industries as they have high stability at elevated temperatures and resistant to chemical denaturation. Geobacillus thermodenitrificans IBRL-nra exploited in this study was originally isolated from a hot spring in Labok, Kelantan, Malaysia with growth temperatures ranging from 45ºC to 70ºC. The production of thermostable lipase by G. thermodenitrificans IBRL-nra at 65˚C was checked qualitatively by streaking the bacteria on lipase screening agar plates. The production of extracellular thermostable lipase by G. thermodenitrificans IBRL-nra was carried out in a shake flask system and 5L stirred-tank bioreactor. The production of thermostable lipase in stirred-tank bioreactor was improved five fold compared to the production in shake flask system and an increment of three fold was observed in the cell growth. 2015-04 Thesis http://eprints.usm.my/32270/ http://eprints.usm.my/32270/1/ANURADHA_BALAN.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Balan, Anuradha Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra
description	Sejak kebelakangan ini, lipase daripada mikroorganisma termofilik telah menjadi tumpuan yang istimewa disebabkan kepelbagaian aplikasinya dalam sektor industri. Ini disebabkan oleh cirinya yang mempunyai kestabilan yang tinggi pada suhu yang tinggi dan tahan penyahaslian oleh bahan kimia. Geobacillus thermodenitrificans IBRL-nra yang digunakan dalam penyelidikan ini telah dipencilkan dari kolam air panas di Labok, Kelantan, Malaysia dan mempunyai suhu pertumbuhan antara 45ºC ke 70ºC. Penghasilan lipase stabil haba daripada G. thermodenitrificans IBRL-nra pada suhu 65˚C telah dikaji secara kualitatif diatas plat agar penyaringan lipase. Penghasilan lipase stabil haba ekstrasel oleh G. thermodenitrificans IBRL-nra telah dijalankan di dalam sistem kelalang goncangan dan bioreaktor tangki teraduk 5L. Penghasilan lipase stabil haba dan pertumbuhan sel di dalam bioreaktor tangki teraduk telah meningkat sebanyak 5 kali ganda dan 3 kali ganda, masing-masing daripada penghasilannya di dalam sistem kelalang goncangan. Lipases from thermophiles have gained interest in recent years as they have various applications in industries. They play significant roles in industries as they have high stability at elevated temperatures and resistant to chemical denaturation. Geobacillus thermodenitrificans IBRL-nra exploited in this study was originally isolated from a hot spring in Labok, Kelantan, Malaysia with growth temperatures ranging from 45ºC to 70ºC. The production of thermostable lipase by G. thermodenitrificans IBRL-nra at 65˚C was checked qualitatively by streaking the bacteria on lipase screening agar plates. The production of extracellular thermostable lipase by G. thermodenitrificans IBRL-nra was carried out in a shake flask system and 5L stirred-tank bioreactor. The production of thermostable lipase in stirred-tank bioreactor was improved five fold compared to the production in shake flask system and an increment of three fold was observed in the cell growth.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Balan, Anuradha
author_facet	Balan, Anuradha
author_sort	Balan, Anuradha
title	Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra
title_short	Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra
title_full	Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra
title_fullStr	Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra
title_full_unstemmed	Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra
title_sort	production, cloning and characterization of thermostable lipase from geobacillus thermodenitrificans ibrl-nra
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2015
url	http://eprints.usm.my/32270/1/ANURADHA_BALAN.pdf
_version_	1747820556927893504

Production, Cloning And Characterization Of Thermostable Lipase From Geobacillus Thermodenitrificans Ibrl-Nra

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