Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus

Tiga puluh enam strain bakteria asid laktik telah diasingkan daripada tenusu, daging dan produk penapaian tempatan. Ekstrak extrasel (CFS) daripada L. plantarum USM8613, yang didapati daripada sosej yang ditapai, menunjukkan aktiviti perencatan dan penghasilan asid laktik yang lebih tinggi (ρ < 0...

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Main Author:	Yong, Cheng Chung
Format:	Thesis
Language:	English
Published:	2016
Subjects:	T1-995 Technology(General)
Online Access:	http://eprints.usm.my/32344/1/YONG_CHENG_CHUNG_24%28NN%29.pdf
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spelling	my-usm-ep.323442019-04-12T05:25:19Z Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus 2016-07 Yong, Cheng Chung T1-995 Technology(General) Tiga puluh enam strain bakteria asid laktik telah diasingkan daripada tenusu, daging dan produk penapaian tempatan. Ekstrak extrasel (CFS) daripada L. plantarum USM8613, yang didapati daripada sosej yang ditapai, menunjukkan aktiviti perencatan dan penghasilan asid laktik yang lebih tinggi (ρ < 0.05) telah dipilih untuk analisis seterusnya. Kajian ex-vivo menunjukkan CFS daripada L. plantarum USM8613 berupaya merencatkan pembentukan biofilem dan pertumbuhan S. aureus pada kulit khinzir. Fraksi yang kaya dengan protein, lemak dan polisakarida yang diekstrak daripada CFS L. plantarum USM8613 telah diuji untuk aktiviti perencatan. Kajian menunjukkan fraksi yang kaya dengan protein memberi kesan yang lebih ketara berbanding dengan fraksi yang kaya lemak and polisakarida. Fraksi yang kaya dengan protein dari L. plantarum USM8613 juga berupaya merencatkan (ρ <0.05) pertumbuhan S. aureus klinikal dan pembentukan biofilem pada kulit khinzir. Rawatan topikal yang mengandungi 800 AU/mL fraksi yang kaya dengna protein daripada L. plantarum USM8613 didapati berupaya mengurangkan (ρ < 0.05) bilangan sel S. aureus ditapak luka tikus. Thirty-six strains of lactic acid bacteria were isolated from local dairy, meat and fermented products. Cell-free-supernatant (CFS) of L. plantarum USM8613, isolated from fermented sausage, was selected for subsequent analyses. The CFS exhibited a significantly stronger (ρ < 0.05) inhibitory activity against S. aureus and produced a higher amount of lactic acid as compared to all strains studied. Ex-vivo study demonstrated CFS from L. plantarum USM8613 inhibited the growth and biofilm formation of S. aureus on porcine skins. CFS of L. plantarum USM8613 was fractionated into protein-rich, lipid-rich and polysaccharide-rich fractions, and all fractions exhibited significant inhibitory activity, with a more prevalent effect from the protein-rich fraction. The antimicrobial and anti-biofilm effects of the protein-rich fraction were further confirmed with S. aureus-infected porcine skins. Topical application of ointment containing 800 AU/mL of the protein-rich fraction from L. plantarum USM8613 significantly reduced (ρ < 0.05) the cell counts of S. aureus in the wound site of S. aureus infected-rats. 2016-07 Thesis http://eprints.usm.my/32344/ http://eprints.usm.my/32344/1/YONG_CHENG_CHUNG_24%28NN%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Teknologi Industri (School of Industrial Technology)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T1-995 Technology(General)
spellingShingle	T1-995 Technology(General) Yong, Cheng Chung Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus
description	Tiga puluh enam strain bakteria asid laktik telah diasingkan daripada tenusu, daging dan produk penapaian tempatan. Ekstrak extrasel (CFS) daripada L. plantarum USM8613, yang didapati daripada sosej yang ditapai, menunjukkan aktiviti perencatan dan penghasilan asid laktik yang lebih tinggi (ρ < 0.05) telah dipilih untuk analisis seterusnya. Kajian ex-vivo menunjukkan CFS daripada L. plantarum USM8613 berupaya merencatkan pembentukan biofilem dan pertumbuhan S. aureus pada kulit khinzir. Fraksi yang kaya dengan protein, lemak dan polisakarida yang diekstrak daripada CFS L. plantarum USM8613 telah diuji untuk aktiviti perencatan. Kajian menunjukkan fraksi yang kaya dengan protein memberi kesan yang lebih ketara berbanding dengan fraksi yang kaya lemak and polisakarida. Fraksi yang kaya dengan protein dari L. plantarum USM8613 juga berupaya merencatkan (ρ <0.05) pertumbuhan S. aureus klinikal dan pembentukan biofilem pada kulit khinzir. Rawatan topikal yang mengandungi 800 AU/mL fraksi yang kaya dengna protein daripada L. plantarum USM8613 didapati berupaya mengurangkan (ρ < 0.05) bilangan sel S. aureus ditapak luka tikus. Thirty-six strains of lactic acid bacteria were isolated from local dairy, meat and fermented products. Cell-free-supernatant (CFS) of L. plantarum USM8613, isolated from fermented sausage, was selected for subsequent analyses. The CFS exhibited a significantly stronger (ρ < 0.05) inhibitory activity against S. aureus and produced a higher amount of lactic acid as compared to all strains studied. Ex-vivo study demonstrated CFS from L. plantarum USM8613 inhibited the growth and biofilm formation of S. aureus on porcine skins. CFS of L. plantarum USM8613 was fractionated into protein-rich, lipid-rich and polysaccharide-rich fractions, and all fractions exhibited significant inhibitory activity, with a more prevalent effect from the protein-rich fraction. The antimicrobial and anti-biofilm effects of the protein-rich fraction were further confirmed with S. aureus-infected porcine skins. Topical application of ointment containing 800 AU/mL of the protein-rich fraction from L. plantarum USM8613 significantly reduced (ρ < 0.05) the cell counts of S. aureus in the wound site of S. aureus infected-rats.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Yong, Cheng Chung
author_facet	Yong, Cheng Chung
author_sort	Yong, Cheng Chung
title	Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus
title_short	Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus
title_full	Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus
title_fullStr	Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus
title_full_unstemmed	Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus
title_sort	effects of protein-rich fraction from lactobacillus plantarum usm8613 against dermal staphylococcus aureus
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri (School of Industrial Technology)
publishDate	2016
url	http://eprints.usm.my/32344/1/YONG_CHENG_CHUNG_24%28NN%29.pdf
_version_	1747820567954718720

Effects Of Protein-Rich Fraction From Lactobacillus Plantarum Usm8613 Against Dermal Staphylococcus Aureus

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