Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies

CYP2D6 mempakan salah satu enzim CYP utama dania bertanggungjawab dalam metabolisma kira-kira 20% daripada ubat-ubatan yang terdapat pada masa kini. CY2D6 adalah berpolymorfisma dan CYP2D6* 10 mempakan alelle kebiasaan di kalangan populasi Malaysia. Dat1. berkenaan interaksi herbal tempatan-ubata...

Full description

Saved in:

Bibliographic Details
Main Author:	Lee, Wee Leng
Format:	Thesis
Language:	English
Published:	2013
Subjects:	RM300-666 Drugs and their actions
Online Access:	http://eprints.usm.my/36415/1/Dr._Lee_Wee_Leng_TSTZ_%28NN24%29.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.36415
record_format	uketd_dc
spelling	my-usm-ep.364152019-04-12T05:26:17Z Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies 2013-04 Lee, Wee Leng RM300-666 Drugs and their actions CYP2D6 mempakan salah satu enzim CYP utama dania bertanggungjawab dalam metabolisma kira-kira 20% daripada ubat-ubatan yang terdapat pada masa kini. CY2D6 adalah berpolymorfisma dan CYP2D6* 10 mempakan alelle kebiasaan di kalangan populasi Malaysia. Dat1. berkenaan interaksi herbal tempatan-ubatan adalah kekurangan. Objektif penyelidikan ini adalah menghasilkan assei in vitro berkenaan interaski ubatan-herbal dan mengunakannya untuk menganalisasi interaksi herbal tempatan. CYP2D61 rekombinan telah dihasilkan dan diekpreskan bersama CYP-reductase dalam bacteria E. coli. CYP2D6 10 dihasilkan dengan kaedah "sitedirected mutagenesis" dan diekpreskan sebagai CYP2D6* 1. CYP2D6 is one of the maJor CYPs enzymes and is responsible for the metabolism of about 20% of cunently available therapeutic chugs. CYP2D6 is polymorphic and CYP2D6* 10 is the common allele in the Malaysian population. There is lack of data on local herbal-drug interaction. The objective of this study was to generate an in vitro drug-herbs int1eraction assay and use it to analyse local herb interaction. Recombinant CYP2D6* 1 was generated and co-expressed with CYPreductase in E. coli. Recombinant CYP2D610 was generated using site-directed mutagenesis and expressed as CYP2D6 1. 2013-04 Thesis http://eprints.usm.my/36415/ http://eprints.usm.my/36415/1/Dr._Lee_Wee_Leng_TSTZ_%28NN24%29.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Perubatan (School of Medical Sciences)
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RM300-666 Drugs and their actions
spellingShingle	RM300-666 Drugs and their actions Lee, Wee Leng Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies
description	CYP2D6 mempakan salah satu enzim CYP utama dania bertanggungjawab dalam metabolisma kira-kira 20% daripada ubat-ubatan yang terdapat pada masa kini. CY2D6 adalah berpolymorfisma dan CYP2D6* 10 mempakan alelle kebiasaan di kalangan populasi Malaysia. Dat1. berkenaan interaksi herbal tempatan-ubatan adalah kekurangan. Objektif penyelidikan ini adalah menghasilkan assei in vitro berkenaan interaski ubatan-herbal dan mengunakannya untuk menganalisasi interaksi herbal tempatan. CYP2D61 rekombinan telah dihasilkan dan diekpreskan bersama CYP-reductase dalam bacteria E. coli. CYP2D6 10 dihasilkan dengan kaedah "sitedirected mutagenesis" dan diekpreskan sebagai CYP2D6* 1. CYP2D6 is one of the maJor CYPs enzymes and is responsible for the metabolism of about 20% of cunently available therapeutic chugs. CYP2D6 is polymorphic and CYP2D6* 10 is the common allele in the Malaysian population. There is lack of data on local herbal-drug interaction. The objective of this study was to generate an in vitro drug-herbs int1eraction assay and use it to analyse local herb interaction. Recombinant CYP2D6* 1 was generated and co-expressed with CYPreductase in E. coli. Recombinant CYP2D610 was generated using site-directed mutagenesis and expressed as CYP2D6 1.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Lee, Wee Leng
author_facet	Lee, Wee Leng
author_sort	Lee, Wee Leng
title	Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies
title_short	Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies
title_full	Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies
title_fullStr	Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies
title_full_unstemmed	Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies
title_sort	cloning and expression of cyp2d61 and cyp2d610 and its application in vitro drug-herbs interaction studies
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan (School of Medical Sciences)
publishDate	2013
url	http://eprints.usm.my/36415/1/Dr._Lee_Wee_Leng_TSTZ_%28NN24%29.pdf
_version_	1747820635341455360

Cloning and expression of CYP2D6*1 and CYP2D6*10 and its application in vitro drug-herbs interaction studies

Similar Items

Cloning and expression of CYP2D61 and CYP2D610 and its application in vitro drug-herbs interaction studies