Comparison of level of endothelial progenitor cells (epcs) in astrocytoma to adjacent normal brain tissue
Introduction: Astrocytomas are the most common primary brain tumors which account for up to two third of all tumors of glial origin and especially the high grade astrocytoma are known to be highly vascularized. Previous studies suggest that vascularization of astrocytoma especially high grade ast...
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| Format: | Thesis |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://eprints.usm.my/39483/1/Dr._Ch%27ng_Chee_How_%28_Neurosurgery_%29-24_pages.pdf |
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| Summary: | Introduction: Astrocytomas are the most common primary brain tumors which
account for up to two third of all tumors of glial origin and especially the high grade
astrocytoma are known to be highly vascularized. Previous studies suggest that
vascularization of astrocytoma especially high grade astrocytoma, is contributed by several
mode of vascularization which explained, the resistance of the tumor to anti-angiogenic
therapy. Endothelial progenitor cells are believed to play an important role in
vascularization of astrocytoma, directly by vasculogenesis or indirectly by secreting various
proangiogenic factors and possible next target of therapy.
Objective: To compare the EPCs level in between the astrocytoma tissue samples
to the adjacent normal brain tissue and subanalysed, by comparing the EPCs level in high
grade astrocytoma to low grade astrocytoma.
Methods: A total of 17 patients with astrocytoma, 11 patients with high grade
astrocytoma (WHO Grade III and IV) and 6 patients with low grade astrocytoma (WHO
Grade I and II), were recruited from Hospital Universiti Sains Malaysia. All patients
underwent surgery for astrocytoma, tumor and normal adjacent brain tissues were collected.
Tumor and normal adjacent brain tissues were sliced and stained with CD 133 and
VEGFR-2 marker and followed by immunohistochemical analysis to determine the EPCs in
the tumor and normal adjacent brain tissue.
Results: The levels of EPCs were significantly higher in the astrocytoma compared
to adjacent normal brain tissue. Sub analysis of the study, compared the level of EPCs in
the high grade astrocytoma to the low grade astrocytoma, do not showed significant
difference.
Conclusions: This study showed higher level of EPCs in the astrocytoma as
compared to the normal adjacent brain tissue suggest the possibility of EPCs play a role in
astrocytoma vascularizatio
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