Development of decision support system for identification of medically important enterobacteriaceae

Members of the family Enterobacteriaceae are the majority of gram-negative organisms identified in a clinical microbiology Laboratory. The family now has over 20 genera and more than 100 species, of which about 50 are associated with human disease. Currently, in Laboratory of Microbiology and Parasi...

Full description

Saved in:

Bibliographic Details
Main Author:	Abdul Latiff, Nur Amalina
Format:	Thesis
Language:	English
Published:	2015
Subjects:	R Medicine (General)
Online Access:	http://eprints.usm.my/40682/1/Dr._Nur_Amalina_Abdul_Latiff-24_pages.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.40682
record_format	uketd_dc
spelling	my-usm-ep.406822018-07-16T01:22:52Z Development of decision support system for identification of medically important enterobacteriaceae 2015 Abdul Latiff, Nur Amalina R Medicine (General) Members of the family Enterobacteriaceae are the majority of gram-negative organisms identified in a clinical microbiology Laboratory. The family now has over 20 genera and more than 100 species, of which about 50 are associated with human disease. Currently, in Laboratory of Microbiology and Parasitology, of Hospital Universiti Sains Malaysia, the identification of Enterobacteriaceae is utilised routinely by conventional biochemical tests. Other than that, commercial system such as API 20E and Vitek 2 automated system are also been utilised specifically for identification of critical samples, due to its expensive cost. Identification manually by conventional method prone to human error during mixing and matching biochemical tests, which further cause misidentification, while identification using commercial methods require high cost. To overcome this problem, there is a need to develop a computerised decision support system to assist microbiologists for identification of Enterobacteriaceae. Decision support system of Enterobacteriaceae (DECIDER) were developed using free open source software, PHP and MySQL by following open source software development methodology. The newly develop system has been compared to previous method; conventional manual system, API 20E system and VITEK 2 automated system by back tested using a total of 356 positive blood culture previous record in year 2011 gathered from Laboratory of Microbiology and Parasitology. Percentage agreement was calculated. The highest percentage of complete agreement was by comparing DECIDER and Vitek 2, with 82 (87.23%) correctly identified organisms. Manual conventional system compared with DECIDER yield about 274 (76.97%) complete agreement for correctly identified organisms. Result has shown that DECIDER, identified a highly acceptable level of identification accuracy for members of the family Enterobacteriaceae. The system is simple and provides ease of use for user. 2015 Thesis http://eprints.usm.my/40682/ http://eprints.usm.my/40682/1/Dr._Nur_Amalina_Abdul_Latiff-24_pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abdul Latiff, Nur Amalina Development of decision support system for identification of medically important enterobacteriaceae
description	Members of the family Enterobacteriaceae are the majority of gram-negative organisms identified in a clinical microbiology Laboratory. The family now has over 20 genera and more than 100 species, of which about 50 are associated with human disease. Currently, in Laboratory of Microbiology and Parasitology, of Hospital Universiti Sains Malaysia, the identification of Enterobacteriaceae is utilised routinely by conventional biochemical tests. Other than that, commercial system such as API 20E and Vitek 2 automated system are also been utilised specifically for identification of critical samples, due to its expensive cost. Identification manually by conventional method prone to human error during mixing and matching biochemical tests, which further cause misidentification, while identification using commercial methods require high cost. To overcome this problem, there is a need to develop a computerised decision support system to assist microbiologists for identification of Enterobacteriaceae. Decision support system of Enterobacteriaceae (DECIDER) were developed using free open source software, PHP and MySQL by following open source software development methodology. The newly develop system has been compared to previous method; conventional manual system, API 20E system and VITEK 2 automated system by back tested using a total of 356 positive blood culture previous record in year 2011 gathered from Laboratory of Microbiology and Parasitology. Percentage agreement was calculated. The highest percentage of complete agreement was by comparing DECIDER and Vitek 2, with 82 (87.23%) correctly identified organisms. Manual conventional system compared with DECIDER yield about 274 (76.97%) complete agreement for correctly identified organisms. Result has shown that DECIDER, identified a highly acceptable level of identification accuracy for members of the family Enterobacteriaceae. The system is simple and provides ease of use for user.
format	Thesis
qualification_level	Master's degree
author	Abdul Latiff, Nur Amalina
author_facet	Abdul Latiff, Nur Amalina
author_sort	Abdul Latiff, Nur Amalina
title	Development of decision support system for identification of medically important enterobacteriaceae
title_short	Development of decision support system for identification of medically important enterobacteriaceae
title_full	Development of decision support system for identification of medically important enterobacteriaceae
title_fullStr	Development of decision support system for identification of medically important enterobacteriaceae
title_full_unstemmed	Development of decision support system for identification of medically important enterobacteriaceae
title_sort	development of decision support system for identification of medically important enterobacteriaceae
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2015
url	http://eprints.usm.my/40682/1/Dr._Nur_Amalina_Abdul_Latiff-24_pages.pdf
_version_	1747820802278948864

Development of decision support system for identification of medically important enterobacteriaceae

Similar Items