Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis

Recent advances in molecular typing provide a robust tool to analyse the heterogeneity of Mycobacterium tuberculosis strains as well as their transmission patterns. In the first attempt study from Kelantan, Malaysia, the frequency and diversity of M. tuberculosis genotypes associated with extrapulmo...

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Main Author:	Ayub, Nur Izzah Farakhin
Format:	Thesis
Language:	English
Published:	2015
Subjects:	RC306-320.5 Tuberculosis
Online Access:	http://eprints.usm.my/40735/1/Dr._Nur_Izzah_Farakhin_Ayub-24_pages.pdf
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id	my-usm-ep.40735
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spelling	my-usm-ep.407352018-07-16T01:12:42Z Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis 2015 Ayub, Nur Izzah Farakhin RC306-320.5 Tuberculosis Recent advances in molecular typing provide a robust tool to analyse the heterogeneity of Mycobacterium tuberculosis strains as well as their transmission patterns. In the first attempt study from Kelantan, Malaysia, the frequency and diversity of M. tuberculosis genotypes associated with extrapulmonary tuberculosis (EPTB) were performed using spoligotyping. Genotyping results were compared with the SITVIT Web, a largest M. tuberculosis molecular markers international database containing 7104 spoligotype patterns worldwide. The results showed that 74.6 % (n = 44) of the isolates were grouped under previously defined shared international type (SIT) numbers while 25.4 % (n = 15) did not have any SIT numbers, thus designated as orphan strains. The largest lineage comprised of 23 isolates (38.9 %) belonging to the Beijing lineage. Present study suggested frequency of M. tuberculosis lineages are related more on geographical settings rather than sites of infection. Bulk of the strains associated with EPTB in the study population also caused by new strain that is not available in the SITVIT Web database. The study may serves as a reference material for further genotyping studies on estimating the actual prevalence of M. tuberculosis associated with EPTB in Malaysia generally 2015 Thesis http://eprints.usm.my/40735/ http://eprints.usm.my/40735/1/Dr._Nur_Izzah_Farakhin_Ayub-24_pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC306-320.5 Tuberculosis
spellingShingle	RC306-320.5 Tuberculosis Ayub, Nur Izzah Farakhin Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis
description	Recent advances in molecular typing provide a robust tool to analyse the heterogeneity of Mycobacterium tuberculosis strains as well as their transmission patterns. In the first attempt study from Kelantan, Malaysia, the frequency and diversity of M. tuberculosis genotypes associated with extrapulmonary tuberculosis (EPTB) were performed using spoligotyping. Genotyping results were compared with the SITVIT Web, a largest M. tuberculosis molecular markers international database containing 7104 spoligotype patterns worldwide. The results showed that 74.6 % (n = 44) of the isolates were grouped under previously defined shared international type (SIT) numbers while 25.4 % (n = 15) did not have any SIT numbers, thus designated as orphan strains. The largest lineage comprised of 23 isolates (38.9 %) belonging to the Beijing lineage. Present study suggested frequency of M. tuberculosis lineages are related more on geographical settings rather than sites of infection. Bulk of the strains associated with EPTB in the study population also caused by new strain that is not available in the SITVIT Web database. The study may serves as a reference material for further genotyping studies on estimating the actual prevalence of M. tuberculosis associated with EPTB in Malaysia generally
format	Thesis
qualification_level	Master's degree
author	Ayub, Nur Izzah Farakhin
author_facet	Ayub, Nur Izzah Farakhin
author_sort	Ayub, Nur Izzah Farakhin
title	Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis
title_short	Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis
title_full	Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis
title_fullStr	Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis
title_full_unstemmed	Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis
title_sort	molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2015
url	http://eprints.usm.my/40735/1/Dr._Nur_Izzah_Farakhin_Ayub-24_pages.pdf
_version_	1747820810888806400

Molecular genotyping of mycobacterium tuberculosis associated with extrapulmonary tuberculosis

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