Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan

Diabetes is one of the chronic diseases affecting worldwide population. Presently, there is a growing interest in herbal remedies for diabetic patient. Therefore, an investigation based on ethnomedicine is required in determined alternative approaches to treat diabetics, such as herbal. The main...

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Main Author: Hamzah, Nurraihana
Format: Thesis
Language:English
Published: 2015
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Online Access:http://eprints.usm.my/40761/1/Dr._Nurraihana_Hamzah-24_pages.pdf
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spelling my-usm-ep.407612018-07-16T01:17:16Z Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan 2015 Hamzah, Nurraihana RM Therapeutics. Pharmacology Diabetes is one of the chronic diseases affecting worldwide population. Presently, there is a growing interest in herbal remedies for diabetic patient. Therefore, an investigation based on ethnomedicine is required in determined alternative approaches to treat diabetics, such as herbal. The main objective of this study was to document ethnomedical of medicinal plants used by aborigines in Gua Musang, Kelantan. The phytochemical, phytonutrient and biological activities of selected plants were screened. Ethnomedical information was collected by interviewing the aboriginal households (house-to-house interviews) and traditional healers in the village. The antidiabetic potential of aqueous extract of selected medicinal plants was determined using α-glucosidase and α-amylase inhibition assay. Phytochemical and phytonutrient were quantitatively determined using standard procedure and antioxidant activities were determined using 2,2’-azinobis(3- ethylbenzothiazoline-6-sulphonic) asid (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ferric reducing/antioksidan power (FRAP) assays. The ethnomedicinal data revealed that 46 plant species were used in treating various types of health problems, from common diseases such as muscle aches and fever to chronic diseases such as diabetes, hypertension and malaria. From the species recorded, three plants used to treat diabetes is Albizia myriophylla, Oxalis barrelieri and Tacca cristata. Therefore, those plants were term as antidiabetic plants in this study. All extracts were found to have significant antidiabetic activities. A. myriophylla extract showed the highest inhibitory activity against α-amylase in which IC50 15.05 μg/ml, while O. barrelieri showed the highest inhibitory activity against α-glucosidase in which IC50 52.40 μg/ml. O. barrelieri showed the highest phenolic content (64.30 ± 1.50 mg GAE/g), flavonoid content (19.29 ± 2.90 mg CE/g), tannin (42.59 ± 10.23 mg TAE/g), alkaloid (3.27 ± 0.33%), fat (1.47 ± 0.60%) and protein (10.61 ± 0.72%) while T. cristata showed the highest content of saponin (7.17 ± 1.15), ash (10.25 ± 0.15%), carbohydrate (53.51 ± 0.94%) and gross energy (240.93 ± 1.74 kcal/100g). Mineral analysis indicates higher concentrations of magnesium, sodium, calcium, mangan, ferum and zinc in T. cristata while higher concentrations of potassium and phosphorus in O. barrelieri. O. barrelieri extract also had the highest antioxidant acivities in ABTS, DPPH and FRAP assays in which 205.95 μmol Trolox/g, 110.41 μmol Trolox/g and 220.93 μmol Trolox/g were obtained, respectively. Further, the most potent extract which is O. barrelieri was subjected to functional beverage development for antidiabetic study in rat model. The results revealed that the O. barrelieri juice showed blood glucose lowering effects in STZ-induced diabetic rats. In conclusion, A. myriophylla, O. barrelieri and T. cristata were found to possess significant in vitro antidiabetic and antioxidant activities. Besides that, O. barrelieri juice showed antidiabetic potential in rat animal models. 2015 Thesis http://eprints.usm.my/40761/ http://eprints.usm.my/40761/1/Dr._Nurraihana_Hamzah-24_pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RM Therapeutics
Pharmacology
spellingShingle RM Therapeutics
Pharmacology
Hamzah, Nurraihana
Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan
description Diabetes is one of the chronic diseases affecting worldwide population. Presently, there is a growing interest in herbal remedies for diabetic patient. Therefore, an investigation based on ethnomedicine is required in determined alternative approaches to treat diabetics, such as herbal. The main objective of this study was to document ethnomedical of medicinal plants used by aborigines in Gua Musang, Kelantan. The phytochemical, phytonutrient and biological activities of selected plants were screened. Ethnomedical information was collected by interviewing the aboriginal households (house-to-house interviews) and traditional healers in the village. The antidiabetic potential of aqueous extract of selected medicinal plants was determined using α-glucosidase and α-amylase inhibition assay. Phytochemical and phytonutrient were quantitatively determined using standard procedure and antioxidant activities were determined using 2,2’-azinobis(3- ethylbenzothiazoline-6-sulphonic) asid (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ferric reducing/antioksidan power (FRAP) assays. The ethnomedicinal data revealed that 46 plant species were used in treating various types of health problems, from common diseases such as muscle aches and fever to chronic diseases such as diabetes, hypertension and malaria. From the species recorded, three plants used to treat diabetes is Albizia myriophylla, Oxalis barrelieri and Tacca cristata. Therefore, those plants were term as antidiabetic plants in this study. All extracts were found to have significant antidiabetic activities. A. myriophylla extract showed the highest inhibitory activity against α-amylase in which IC50 15.05 μg/ml, while O. barrelieri showed the highest inhibitory activity against α-glucosidase in which IC50 52.40 μg/ml. O. barrelieri showed the highest phenolic content (64.30 ± 1.50 mg GAE/g), flavonoid content (19.29 ± 2.90 mg CE/g), tannin (42.59 ± 10.23 mg TAE/g), alkaloid (3.27 ± 0.33%), fat (1.47 ± 0.60%) and protein (10.61 ± 0.72%) while T. cristata showed the highest content of saponin (7.17 ± 1.15), ash (10.25 ± 0.15%), carbohydrate (53.51 ± 0.94%) and gross energy (240.93 ± 1.74 kcal/100g). Mineral analysis indicates higher concentrations of magnesium, sodium, calcium, mangan, ferum and zinc in T. cristata while higher concentrations of potassium and phosphorus in O. barrelieri. O. barrelieri extract also had the highest antioxidant acivities in ABTS, DPPH and FRAP assays in which 205.95 μmol Trolox/g, 110.41 μmol Trolox/g and 220.93 μmol Trolox/g were obtained, respectively. Further, the most potent extract which is O. barrelieri was subjected to functional beverage development for antidiabetic study in rat model. The results revealed that the O. barrelieri juice showed blood glucose lowering effects in STZ-induced diabetic rats. In conclusion, A. myriophylla, O. barrelieri and T. cristata were found to possess significant in vitro antidiabetic and antioxidant activities. Besides that, O. barrelieri juice showed antidiabetic potential in rat animal models.
format Thesis
qualification_level Master's degree
author Hamzah, Nurraihana
author_facet Hamzah, Nurraihana
author_sort Hamzah, Nurraihana
title Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan
title_short Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan
title_full Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan
title_fullStr Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan
title_full_unstemmed Kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di Gua Musang, Kelantan
title_sort kajian etnoperubatan, analisis fitokimia dan aktiviti biologi tumbuh-tumbuhan antidiabetik terpilih yang digunakan oleh masyarakat orang asli di gua musang, kelantan
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Perubatan
publishDate 2015
url http://eprints.usm.my/40761/1/Dr._Nurraihana_Hamzah-24_pages.pdf
_version_ 1747820817243176960