Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum

Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum

Apoptosis makrofaj merupakan mekanisme yang berkesan dalam mengawal jangkitan intrasel semasa gerak balas imun semulajadi terhadap pelbagai patogen termasuk parasit malaria. Kajian ini dijalankan untuk menentukan aktiviti apoptosis dan pengekspresan protein pengaktifan mitogen (MAPK) dalam sel ma...

Full description

Saved in:
Bibliographic Details
Main Author: Zulkipli, Anis Fadhilah
Format: Thesis
Language:English
Published: 2016
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/40846/1/Dr._Anis_Fadhilah__Zulkipli_TSTZ.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-usm-ep.40846
record_format uketd_dc
spelling my-usm-ep.408462018-06-28T08:19:16Z Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum 2016-12 Zulkipli, Anis Fadhilah R5-920 Medicine (General) Apoptosis makrofaj merupakan mekanisme yang berkesan dalam mengawal jangkitan intrasel semasa gerak balas imun semulajadi terhadap pelbagai patogen termasuk parasit malaria. Kajian ini dijalankan untuk menentukan aktiviti apoptosis dan pengekspresan protein pengaktifan mitogen (MAPK) dalam sel makrofaj mencit J774A.1 yang dijangkiti klon BCG dan BCG rekombinan (rBCG) yang mengekspreskan terminus C protein permukaan merozoite-1 (MSP-1C) daripada Plasmodium falciparum selama 48 jam. Pewarnaan nukleus menggunakan Hoest 33342 menunjukkan bahawa klon rBCG berupaya meningkatkan kondensasi nuklear dan peringkat morfologi apoptosis dalam sel makrofaj yang dijangkiti berbanding BCG dan LPS. Macrophage apoptosis exerts an efficient mechanism in controlling intracellular infection during innate immune response against various pathogens including malaria parasites. This study was carried out to determine the apoptosis activity and mitogen activated protein kinase (MAPK) expression in mouse macrophage cell line J774A.1 infected with a Mycobacterium bovis bacille Calmette-Guerin (BCG) clone and a recombinant BCG (rBCG) clone expressing the C-terminus of merozoite surface protein-1 (MSP-1C) of Plasmodium falciparum for 48 hours. The nuclear staining with Hoechst 33342 showed that the rBCG clone was capable of increasing the nuclear condensation and morphological stages of apoptosis in the infected cells compared to the BCG-infected cells and the LPS-stimulated cells. The flow cytometric analysis using Annexin-V and PI staining confirmed that the rBCG clone significantly increased the percentage of early apoptotic activity in the infected macrophage higher than the one stimulated by the parent BCG clone and LPS. 2016-12 Thesis http://eprints.usm.my/40846/ http://eprints.usm.my/40846/1/Dr._Anis_Fadhilah__Zulkipli_TSTZ.pdf application/pdf en public masters USM Pusat Pengajian Sains Kesihatan (School of Health Sciences)
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Zulkipli, Anis Fadhilah
Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum
description Apoptosis makrofaj merupakan mekanisme yang berkesan dalam mengawal jangkitan intrasel semasa gerak balas imun semulajadi terhadap pelbagai patogen termasuk parasit malaria. Kajian ini dijalankan untuk menentukan aktiviti apoptosis dan pengekspresan protein pengaktifan mitogen (MAPK) dalam sel makrofaj mencit J774A.1 yang dijangkiti klon BCG dan BCG rekombinan (rBCG) yang mengekspreskan terminus C protein permukaan merozoite-1 (MSP-1C) daripada Plasmodium falciparum selama 48 jam. Pewarnaan nukleus menggunakan Hoest 33342 menunjukkan bahawa klon rBCG berupaya meningkatkan kondensasi nuklear dan peringkat morfologi apoptosis dalam sel makrofaj yang dijangkiti berbanding BCG dan LPS. Macrophage apoptosis exerts an efficient mechanism in controlling intracellular infection during innate immune response against various pathogens including malaria parasites. This study was carried out to determine the apoptosis activity and mitogen activated protein kinase (MAPK) expression in mouse macrophage cell line J774A.1 infected with a Mycobacterium bovis bacille Calmette-Guerin (BCG) clone and a recombinant BCG (rBCG) clone expressing the C-terminus of merozoite surface protein-1 (MSP-1C) of Plasmodium falciparum for 48 hours. The nuclear staining with Hoechst 33342 showed that the rBCG clone was capable of increasing the nuclear condensation and morphological stages of apoptosis in the infected cells compared to the BCG-infected cells and the LPS-stimulated cells. The flow cytometric analysis using Annexin-V and PI staining confirmed that the rBCG clone significantly increased the percentage of early apoptotic activity in the infected macrophage higher than the one stimulated by the parent BCG clone and LPS.
format Thesis
qualification_level Master's degree
author Zulkipli, Anis Fadhilah
author_facet Zulkipli, Anis Fadhilah
author_sort Zulkipli, Anis Fadhilah
title Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum
title_short Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum
title_full Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum
title_fullStr Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum
title_full_unstemmed Apoptosis Activity and Mitogen Activated Protein Kinase Expression of the Mouse Macrophage Cell Line J774a.1 Infected with a Recombinant Bcg Expressing the Cterminus of Merozoite Surface Protein-1 of Plasmodium Falciparum
title_sort apoptosis activity and mitogen activated protein kinase expression of the mouse macrophage cell line j774a.1 infected with a recombinant bcg expressing the cterminus of merozoite surface protein-1 of plasmodium falciparum
granting_institution USM
granting_department Pusat Pengajian Sains Kesihatan (School of Health Sciences)
publishDate 2016
url http://eprints.usm.my/40846/1/Dr._Anis_Fadhilah__Zulkipli_TSTZ.pdf
_version_ 1747820832704430080

Similar Items