Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia

Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia

Several waves of human migration into Peninsular Malaysia led to the stratification of various ethnic groups. The earliest inhabitants of Peninsular Malaysia are the Orang Asli population, namely Semang, Senoi and Proto Malay. In the present study, we typed the HLA-A, -B and -DRB1 loci of the two...

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Main Author: Abd Razak, Tasnim
Format: Thesis
Language:English
Published: 2015
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RC31-1245 Internal medicine
Online Access:http://eprints.usm.my/41036/1/Dr._Tasnim_Abd_Razak-24_pages.pdf
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spelling my-usm-ep.410362019-04-12T05:25:51Z Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia 2015 Abd Razak, Tasnim RC31-1245 Internal medicine Several waves of human migration into Peninsular Malaysia led to the stratification of various ethnic groups. The earliest inhabitants of Peninsular Malaysia are the Orang Asli population, namely Semang, Senoi and Proto Malay. In the present study, we typed the HLA-A, -B and -DRB1 loci of the two oldest Orang Asli populations, Semang and Senoi. Sequence-based HLA typing was performed on 85 individuals from four Orang Asli subgroups: Kensiu (N = 21) and Bateq (N = 16) of the Semang major group, and Che Wong (N = 10) and Semai (N = 38) of the Senoi major group. A total of 11, 21 and 17 HLA-A, -B and -DRB1 alleles were identified, respectively in the studied Orang Asli subgroups. HLA-A*02:01 was the most common allele in Kensiu, Bateq and Che Wong, and A*24:07 was the most common in Semai. B*18:01 was the most common allele in all of the Orang Asli subgroups. DRB1*09:01 was the most common allele in Kensiu and Che Wong, and DRBI*12:02 was the most common allele in Bateq and Semai. The following twoloci haplotypes were common in the Orang Asli subgroups: HLA-A*02:01-B*18:01, A*02:01-DRB1*09:01, A*02:01-DRB1*12:02, B*15:13-DRB1*12:02 and B*18:01- DRB1*09:01. The three-loci, HLA-A*02:01-B*18:01-DRB1*09:01 haplotype was observed in all the Orang Asli subgroups. The HLA analysis in this study suggested that the Orang Asli subgroups experienced genetic drift based on evidence of limited allelic diversity. Correspondence analysis based on HLA-A, -B and -DRB1 allele frequencies suggested that the Orang Asli subgroups share high genetic affinity with the populations from South China, Indochina, Malaysia, Singapore and Indonesia. Although the HLA data is not conclusive to correlate the Semang with the African population, there was one individual who expressed DRB1*15:03 allele in the Kensiu where this allele is observed in high frequency in Africa. The Semang HLA allelic characteristics showed similar profiles with those of other Southeast Asian populations probably due to evolutionary pressure on the HLA gene complex. The HLA data also demonstrated similar HLA allelic profiles of the Senoi with Indochina/South China populations. In conclusion, HLA allelic profiles of the Orang Asli subgroups can be said to be characteristically Southeast Asian. The establishment of HLA database for selected Orang Asli subgroups may provide useful reference in clinical applications (e.g. cell and organ transplants), HLAdisease association studies, HLA-adverse drug reaction association studies and DNA marker for personal identification and population genetics. 2015 Thesis http://eprints.usm.my/41036/ http://eprints.usm.my/41036/1/Dr._Tasnim_Abd_Razak-24_pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RC31-1245 Internal medicine
spellingShingle RC31-1245 Internal medicine
Abd Razak, Tasnim
Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia
description Several waves of human migration into Peninsular Malaysia led to the stratification of various ethnic groups. The earliest inhabitants of Peninsular Malaysia are the Orang Asli population, namely Semang, Senoi and Proto Malay. In the present study, we typed the HLA-A, -B and -DRB1 loci of the two oldest Orang Asli populations, Semang and Senoi. Sequence-based HLA typing was performed on 85 individuals from four Orang Asli subgroups: Kensiu (N = 21) and Bateq (N = 16) of the Semang major group, and Che Wong (N = 10) and Semai (N = 38) of the Senoi major group. A total of 11, 21 and 17 HLA-A, -B and -DRB1 alleles were identified, respectively in the studied Orang Asli subgroups. HLA-A*02:01 was the most common allele in Kensiu, Bateq and Che Wong, and A*24:07 was the most common in Semai. B*18:01 was the most common allele in all of the Orang Asli subgroups. DRB1*09:01 was the most common allele in Kensiu and Che Wong, and DRBI*12:02 was the most common allele in Bateq and Semai. The following twoloci haplotypes were common in the Orang Asli subgroups: HLA-A*02:01-B*18:01, A*02:01-DRB1*09:01, A*02:01-DRB1*12:02, B*15:13-DRB1*12:02 and B*18:01- DRB1*09:01. The three-loci, HLA-A*02:01-B*18:01-DRB1*09:01 haplotype was observed in all the Orang Asli subgroups. The HLA analysis in this study suggested that the Orang Asli subgroups experienced genetic drift based on evidence of limited allelic diversity. Correspondence analysis based on HLA-A, -B and -DRB1 allele frequencies suggested that the Orang Asli subgroups share high genetic affinity with the populations from South China, Indochina, Malaysia, Singapore and Indonesia. Although the HLA data is not conclusive to correlate the Semang with the African population, there was one individual who expressed DRB1*15:03 allele in the Kensiu where this allele is observed in high frequency in Africa. The Semang HLA allelic characteristics showed similar profiles with those of other Southeast Asian populations probably due to evolutionary pressure on the HLA gene complex. The HLA data also demonstrated similar HLA allelic profiles of the Senoi with Indochina/South China populations. In conclusion, HLA allelic profiles of the Orang Asli subgroups can be said to be characteristically Southeast Asian. The establishment of HLA database for selected Orang Asli subgroups may provide useful reference in clinical applications (e.g. cell and organ transplants), HLAdisease association studies, HLA-adverse drug reaction association studies and DNA marker for personal identification and population genetics.
format Thesis
qualification_level Master's degree
author Abd Razak, Tasnim
author_facet Abd Razak, Tasnim
author_sort Abd Razak, Tasnim
title Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia
title_short Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia
title_full Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia
title_fullStr Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia
title_full_unstemmed Distribution of HLA alleles in the semang and senoi orang asli populations in Peninsular Malaysia
title_sort distribution of hla alleles in the semang and senoi orang asli populations in peninsular malaysia
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Perubatan
publishDate 2015
url http://eprints.usm.my/41036/1/Dr._Tasnim_Abd_Razak-24_pages.pdf
_version_ 1747820866062778368

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