Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris

Human erythropoietin is a hormone or glycoprotein produced by the kidney and bone marrow with important physiological functions, such as, erythropoiesis, angiogenesis, and wound healing. The natural source of huEpo is purified from human urine (uhuEPO). Alternatively, recombinant human erythropoi...

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Main Author:	Bustami, Yazmin
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/41239/1/Yazmin_Bustami-Shahfiq.pdf
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id	my-usm-ep.41239
record_format	uketd_dc
spelling	my-usm-ep.412392018-08-06T07:38:58Z Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris 2010-11 Bustami, Yazmin QH1 Natural history (General - Including nature conservation, geographical distribution) Human erythropoietin is a hormone or glycoprotein produced by the kidney and bone marrow with important physiological functions, such as, erythropoiesis, angiogenesis, and wound healing. The natural source of huEpo is purified from human urine (uhuEPO). Alternatively, recombinant human erythropoietin could overcome the limited supply of uhuEPO. Thus, the focus of this study is to generate a synthetic huEPO gene and express in Pichia pastoris. PCR assembly method utilized to construct a synthetic gene using twenty sets of overlapping oligonucleotides, covering the huEPO A gene sequence and two newly introduced restriction enzyme sites. Results from DNA sequence analysis showed accurate assembled synthetic huEPO gene albeit minor base mutations detected. 2010-11 Thesis http://eprints.usm.my/41239/ http://eprints.usm.my/41239/1/Yazmin_Bustami-Shahfiq.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Bustami, Yazmin Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
description	Human erythropoietin is a hormone or glycoprotein produced by the kidney and bone marrow with important physiological functions, such as, erythropoiesis, angiogenesis, and wound healing. The natural source of huEpo is purified from human urine (uhuEPO). Alternatively, recombinant human erythropoietin could overcome the limited supply of uhuEPO. Thus, the focus of this study is to generate a synthetic huEPO gene and express in Pichia pastoris. PCR assembly method utilized to construct a synthetic gene using twenty sets of overlapping oligonucleotides, covering the huEPO A gene sequence and two newly introduced restriction enzyme sites. Results from DNA sequence analysis showed accurate assembled synthetic huEPO gene albeit minor base mutations detected.
format	Thesis
qualification_level	Master's degree
author	Bustami, Yazmin
author_facet	Bustami, Yazmin
author_sort	Bustami, Yazmin
title	Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
title_short	Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
title_full	Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
title_fullStr	Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
title_full_unstemmed	Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
title_sort	cloning and expression of synthetic human erythropoietin in methylotrophic yeast, pichia pastoris
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2010
url	http://eprints.usm.my/41239/1/Yazmin_Bustami-Shahfiq.pdf
_version_	1747820893452632064

Cloning And Expression Of Synthetic Human Erythropoietin In Methylotrophic Yeast, Pichia Pastoris

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