Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant

In Burkholderia pseudomallei, a pathogen that causes melioidosis, the gene cluster that encodes for capsular polysaccharide are located in chromosome one. There are 22 genes in this cluster and among the capsular genes in the cluster, the wzm gene has not been studied thoroughly. The amino acid s...

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Main Author:	Yuen, Chee Wah
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/41240/1/Yuen_Chee_Wah_Shahfiq.pdf
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spelling	my-usm-ep.412402018-08-06T07:38:36Z Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant 2010-10 Yuen, Chee Wah QH1 Natural history (General - Including nature conservation, geographical distribution) In Burkholderia pseudomallei, a pathogen that causes melioidosis, the gene cluster that encodes for capsular polysaccharide are located in chromosome one. There are 22 genes in this cluster and among the capsular genes in the cluster, the wzm gene has not been studied thoroughly. The amino acid sequence of wzm shows high similarity to CtrC (Neisseria Meningitidis), BexB (Haemophilus Influenzae) and KpsM (Escherichia coli). To study the function of wzm gene, an isogenic mutant of wzm gene was generated and analyzed in comparison with the wild type strain. From the findings, the mutant produced less biofilm compared to the wild type strain. Furthermore, the mutant was more sensitive towards desiccation and oxidative stress than the wild type strain. However, no significant difference was observed for both mutant and wild type strains in their sensitivity towards high osmolarity and acid conditions. Moreover, scanning electron microscopy showed that the bacterial cells of the mutant strain have “rough” surface compared to the wild type strain. However, no phenotypic difference could be observed using transmission electron microscopy when both bacterial cells of both mutant and wild type strains were compared. 2010-10 Thesis http://eprints.usm.my/41240/ http://eprints.usm.my/41240/1/Yuen_Chee_Wah_Shahfiq.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Yuen, Chee Wah Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant
description	In Burkholderia pseudomallei, a pathogen that causes melioidosis, the gene cluster that encodes for capsular polysaccharide are located in chromosome one. There are 22 genes in this cluster and among the capsular genes in the cluster, the wzm gene has not been studied thoroughly. The amino acid sequence of wzm shows high similarity to CtrC (Neisseria Meningitidis), BexB (Haemophilus Influenzae) and KpsM (Escherichia coli). To study the function of wzm gene, an isogenic mutant of wzm gene was generated and analyzed in comparison with the wild type strain. From the findings, the mutant produced less biofilm compared to the wild type strain. Furthermore, the mutant was more sensitive towards desiccation and oxidative stress than the wild type strain. However, no significant difference was observed for both mutant and wild type strains in their sensitivity towards high osmolarity and acid conditions. Moreover, scanning electron microscopy showed that the bacterial cells of the mutant strain have “rough” surface compared to the wild type strain. However, no phenotypic difference could be observed using transmission electron microscopy when both bacterial cells of both mutant and wild type strains were compared.
format	Thesis
qualification_level	Master's degree
author	Yuen, Chee Wah
author_facet	Yuen, Chee Wah
author_sort	Yuen, Chee Wah
title	Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant
title_short	Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant
title_full	Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant
title_fullStr	Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant
title_full_unstemmed	Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant
title_sort	construction and characterization of a burkholderia pseudomallei wzm deletion mutant
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2010
url	http://eprints.usm.my/41240/1/Yuen_Chee_Wah_Shahfiq.pdf
_version_	1747820893693804544

Construction And Characterization Of A Burkholderia Pseudomallei Wzm Deletion Mutant

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