Bioassay Guided Isolation Of Antitermite Compounds From The Bark Of Rhizophora Apiculata
Serbuk kulit kayu Rhizophora apiculata pada mulanya diekstrak dengan petroleum eter (PE) dan metanol. Ekstrak metanol dalam bentuk larutan akueus telah diekstrak semula ke dalam pecahan kloroform (CE), etil asetat (EE) dan n-butanol (BE). Pengasingan berpandukan ujian makmal (ASTM D3345-74) telah...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://eprints.usm.my/41274/1/Kong_Nein_Hing.pdf |
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| Summary: | Serbuk kulit kayu Rhizophora apiculata pada mulanya diekstrak dengan petroleum eter
(PE) dan metanol. Ekstrak metanol dalam bentuk larutan akueus telah diekstrak semula ke
dalam pecahan kloroform (CE), etil asetat (EE) dan n-butanol (BE). Pengasingan berpandukan
ujian makmal (ASTM D3345-74) telah dijalankan dengan mempertimbangkan kehilangan berat
kayu minimum (LWL). Aktiviti anti anai-anainya (ATA) adalah EE>CE>PE>BE, LWLEE =
10.54% pada 500 ppm. Secara perbandingan, kawalan telah haus dimakan. Fraksi-fraksi EE
menunjukkan ATA dalam turutan F2>F3>F1, LWLF2 = 5.53%. Analisis kimia dan spektroskopi
menentukan asid fenolik adalah komponen aktif dalam EE. Fraksi F2 seterusnya diekstrak
dengan kaedah asid-bes (pH ~3) dan hasilnya dikromatografkan. Sifat-sifat kimia dan biologi
tidak berubah selepas pemisahan dan ATAnya adalah F2AII>F2AIII>(F2R≈F2AI), LWLF2AII,
F2AIII = 6.86, 9.12%.
The powdered bark of Rhizophora apiculata was initially extracted with petroleum
ether (PE) and methanol. The aqueous methanol extract was re-extracted into chloroform (CE),
ethyl acetate (EE) and n-butanol (BE). Bioassay (ASTM D3345-74) guided fractionation was
carried out on the basis of the least wood loss (LWL). The anti-termite activity (ATA) was in
the order of EE>CE>PE>BE, LWLEE = 10.54% at 500 ppm. In contrast, controls were severely
damaged. Fractions of EE showed ATA in the order of F2>F3>F1, LWLF2 = 5.53%. Chemical
and spectral studies proposed that the active compounds in EE were of phenolic acids. Fraction
F2 was then partitioned into aqueous and organic layers at pH ~3 and the organic residue was
subjected to chromatographic separation. The chemical and biological properties of the F2
fractions were not altered and the ATA sequence was F2AII>F2AIII>(F2R≈F2AI), LWLF2AII,
F2AIII = 6.86, 9.12%. |
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